Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygjP and ygjQ are separated by 76 nucleotides ygjQ and ygjR are separated by 78 nucleotides ygjR and alx are separated by 282 nucleotides
b3085: ygjP - orf, hypothetical protein (VIMSS), at 3,233,982 to 3,234,485
ygjP
b3086: ygjQ - predicted thioredoxin-like (NCBI), at 3,234,562 to 3,235,254
ygjQ
b3087: ygjR - predicted NAD(P)-binding dehydrogenase (RefSeq), at 3,235,333 to 3,236,319
ygjR
b3088: alx - predicted inner membrane protein, part of terminus (NCBI), at 3,236,602 to 3,237,567
alx
Position (kb)
3235
3236
3237 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3234.341 kb on - strand, within ygjP at 3234.369 kb on + strand, within ygjP at 3234.411 kb on + strand, within ygjP at 3234.411 kb on + strand, within ygjP at 3234.413 kb on - strand, within ygjP at 3234.525 kb on + strand at 3234.544 kb on - strand at 3234.557 kb on + strand at 3234.708 kb on + strand, within ygjQ at 3234.716 kb on - strand, within ygjQ at 3234.793 kb on - strand, within ygjQ at 3234.805 kb on + strand, within ygjQ at 3234.805 kb on + strand, within ygjQ at 3234.813 kb on - strand, within ygjQ at 3234.876 kb on + strand, within ygjQ at 3234.892 kb on - strand, within ygjQ at 3234.892 kb on - strand, within ygjQ at 3235.007 kb on + strand, within ygjQ at 3235.015 kb on - strand, within ygjQ at 3235.105 kb on - strand, within ygjQ at 3235.105 kb on - strand, within ygjQ at 3235.152 kb on - strand, within ygjQ at 3235.229 kb on - strand at 3235.241 kb on - strand at 3235.267 kb on - strand at 3235.339 kb on - strand at 3235.398 kb on - strand at 3235.398 kb on - strand at 3235.444 kb on - strand, within ygjR at 3235.649 kb on + strand, within ygjR at 3235.746 kb on - strand, within ygjR at 3235.746 kb on - strand, within ygjR at 3235.848 kb on + strand, within ygjR at 3235.910 kb on - strand, within ygjR at 3235.910 kb on - strand, within ygjR at 3235.958 kb on + strand, within ygjR at 3235.983 kb on + strand, within ygjR at 3236.005 kb on + strand, within ygjR at 3236.005 kb on + strand, within ygjR at 3236.035 kb on + strand, within ygjR at 3236.066 kb on + strand, within ygjR at 3236.068 kb on - strand, within ygjR at 3236.185 kb on - strand, within ygjR at 3236.222 kb on + strand at 3236.230 kb on - strand at 3236.230 kb on - strand at 3236.241 kb on + strand at 3236.241 kb on + strand at 3236.274 kb on + strand at 3236.429 kb on + strand at 3236.429 kb on + strand at 3236.508 kb on + strand at 3236.563 kb on + strand at 3236.563 kb on + strand at 3236.607 kb on - strand at 3236.620 kb on + strand at 3236.620 kb on + strand at 3236.672 kb on - strand at 3236.701 kb on + strand, within alx at 3236.703 kb on + strand, within alx at 3236.747 kb on - strand, within alx at 3236.752 kb on - strand, within alx at 3236.763 kb on + strand, within alx at 3236.763 kb on + strand, within alx at 3236.763 kb on - strand, within alx at 3236.763 kb on - strand, within alx at 3236.773 kb on + strand, within alx at 3236.773 kb on + strand, within alx at 3236.783 kb on + strand, within alx at 3236.784 kb on - strand, within alx at 3236.816 kb on + strand, within alx at 3236.816 kb on + strand, within alx at 3236.946 kb on + strand, within alx at 3236.961 kb on - strand, within alx at 3236.993 kb on + strand, within alx at 3236.993 kb on + strand, within alx at 3237.052 kb on - strand, within alx at 3237.083 kb on + strand, within alx at 3237.149 kb on + strand, within alx at 3237.152 kb on - strand, within alx at 3237.199 kb on + strand, within alx at 3237.203 kb on + strand, within alx at 3237.293 kb on + strand, within alx at 3237.301 kb on - strand, within alx at 3237.301 kb on - strand, within alx at 3237.318 kb on - strand, within alx at 3237.318 kb on - strand, within alx
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 3,234,341 - ygjP b3085 0.71 +0.7 3,234,369 + ygjP b3085 0.77 -1.3 3,234,411 + ygjP b3085 0.85 -2.0 3,234,411 + ygjP b3085 0.85 +0.9 3,234,413 - ygjP b3085 0.86 +1.6 3,234,525 + +0.1 3,234,544 - -0.4 3,234,557 + -1.3 3,234,708 + ygjQ b3086 0.21 -0.4 3,234,716 - ygjQ b3086 0.22 -0.0 3,234,793 - ygjQ b3086 0.33 +0.7 3,234,805 + ygjQ b3086 0.35 -0.9 3,234,805 + ygjQ b3086 0.35 -1.4 3,234,813 - ygjQ b3086 0.36 -0.5 3,234,876 + ygjQ b3086 0.45 +2.4 3,234,892 - ygjQ b3086 0.48 +2.1 3,234,892 - ygjQ b3086 0.48 +2.0 3,235,007 + ygjQ b3086 0.64 +0.0 3,235,015 - ygjQ b3086 0.65 +2.0 3,235,105 - ygjQ b3086 0.78 +0.3 3,235,105 - ygjQ b3086 0.78 +0.6 3,235,152 - ygjQ b3086 0.85 +1.2 3,235,229 - -0.8 3,235,241 - -0.6 3,235,267 - +1.3 3,235,339 - +1.0 3,235,398 - +1.8 3,235,398 - -1.2 3,235,444 - ygjR b3087 0.11 +0.3 3,235,649 + ygjR b3087 0.32 -1.0 3,235,746 - ygjR b3087 0.42 -0.7 3,235,746 - ygjR b3087 0.42 +2.1 3,235,848 + ygjR b3087 0.52 -2.1 3,235,910 - ygjR b3087 0.58 -0.9 3,235,910 - ygjR b3087 0.58 -0.7 3,235,958 + ygjR b3087 0.63 -0.7 3,235,983 + ygjR b3087 0.66 +0.3 3,236,005 + ygjR b3087 0.68 +0.5 3,236,005 + ygjR b3087 0.68 -1.6 3,236,035 + ygjR b3087 0.71 +0.3 3,236,066 + ygjR b3087 0.74 -0.6 3,236,068 - ygjR b3087 0.74 +0.8 3,236,185 - ygjR b3087 0.86 +0.5 3,236,222 + +0.7 3,236,230 - -0.2 3,236,230 - +0.1 3,236,241 + +1.2 3,236,241 + -0.3 3,236,274 + +0.3 3,236,429 + -1.5 3,236,429 + -0.8 3,236,508 + -1.0 3,236,563 + -1.1 3,236,563 + +2.8 3,236,607 - -0.1 3,236,620 + -0.2 3,236,620 + +0.8 3,236,672 - +1.8 3,236,701 + alx b3088 0.10 -0.0 3,236,703 + alx b3088 0.10 -0.7 3,236,747 - alx b3088 0.15 -0.0 3,236,752 - alx b3088 0.16 +1.5 3,236,763 + alx b3088 0.17 -0.8 3,236,763 + alx b3088 0.17 +2.6 3,236,763 - alx b3088 0.17 -1.9 3,236,763 - alx b3088 0.17 -1.5 3,236,773 + alx b3088 0.18 -0.5 3,236,773 + alx b3088 0.18 +0.5 3,236,783 + alx b3088 0.19 -2.7 3,236,784 - alx b3088 0.19 +1.3 3,236,816 + alx b3088 0.22 +1.8 3,236,816 + alx b3088 0.22 -1.3 3,236,946 + alx b3088 0.36 -1.1 3,236,961 - alx b3088 0.37 -0.6 3,236,993 + alx b3088 0.40 +0.0 3,236,993 + alx b3088 0.40 -0.4 3,237,052 - alx b3088 0.47 +1.8 3,237,083 + alx b3088 0.50 +0.2 3,237,149 + alx b3088 0.57 -1.9 3,237,152 - alx b3088 0.57 +2.5 3,237,199 + alx b3088 0.62 +0.2 3,237,203 + alx b3088 0.62 +0.1 3,237,293 + alx b3088 0.72 -0.5 3,237,301 - alx b3088 0.72 -3.0 3,237,301 - alx b3088 0.72 -0.3 3,237,318 - alx b3088 0.74 -1.6 3,237,318 - alx b3088 0.74 -1.3
Or see this region's nucleotide sequence