Strain Fitness in Escherichia coli BW25113 around b2989

Experiment: 3-methyl-1-butanol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgss and b2989 are separated by 204 nucleotidesb2989 and hybG are separated by 122 nucleotideshybG and hybF are separated by 12 nucleotideshybF and hybE overlap by 8 nucleotides b2988: gss - fused glutathionylspermidine amidase/glutathionylspermidine synthetase (NCBI), at 3,134,685 to 3,136,544 gss b2989: b2989 - orf, hypothetical protein (VIMSS), at 3,136,749 to 3,137,615 b2989 b2990: hybG - hydrogenase 2 accessory protein (NCBI), at 3,137,738 to 3,137,986 hybG b2991: hybF - protein involved with the maturation of hydrogenases 1 and 2 (NCBI), at 3,137,999 to 3,138,340 hybF b2992: hybE - hydrogenase 2-specific chaperone (NCBI), at 3,138,333 to 3,138,821 hybE Position (kb) 3136 3137 3138Strain fitness (log2 ratio) -2 -1 0 1 2at 3135.848 kb on + strand, within gssat 3135.848 kb on + strand, within gssat 3135.902 kb on - strand, within gssat 3135.959 kb on - strand, within gssat 3136.065 kb on + strand, within gssat 3136.072 kb on + strand, within gssat 3136.073 kb on - strand, within gssat 3136.245 kb on + strand, within gssat 3136.254 kb on - strand, within gssat 3136.321 kb on - strand, within gssat 3136.325 kb on - strand, within gssat 3136.325 kb on - strand, within gssat 3136.472 kb on - strandat 3136.472 kb on - strandat 3136.502 kb on - strandat 3136.505 kb on - strandat 3136.505 kb on - strandat 3136.534 kb on + strandat 3136.629 kb on + strandat 3136.629 kb on + strandat 3136.637 kb on - strandat 3136.647 kb on - strandat 3136.648 kb on - strandat 3136.706 kb on + strandat 3136.706 kb on + strandat 3136.729 kb on - strandat 3137.122 kb on - strand, within b2989at 3137.208 kb on + strand, within b2989at 3137.208 kb on + strand, within b2989at 3137.294 kb on + strand, within b2989at 3137.339 kb on - strand, within b2989at 3137.360 kb on + strandat 3137.372 kb on + strand, within b2989at 3137.372 kb on + strand, within b2989at 3137.406 kb on + strand, within b2989at 3137.414 kb on - strand, within b2989at 3137.463 kb on + strand, within b2989at 3137.525 kb on - strand, within b2989at 3137.528 kb on + strand, within b2989at 3137.615 kb on - strandat 3137.615 kb on - strandat 3137.691 kb on - strandat 3137.700 kb on + strandat 3137.714 kb on + strandat 3137.717 kb on - strandat 3137.839 kb on - strand, within hybGat 3137.839 kb on - strand, within hybGat 3137.907 kb on + strand, within hybGat 3137.986 kb on + strandat 3137.986 kb on + strandat 3138.086 kb on + strand, within hybFat 3138.146 kb on - strand, within hybFat 3138.152 kb on + strand, within hybFat 3138.166 kb on + strand, within hybFat 3138.233 kb on - strand, within hybFat 3138.274 kb on - strand, within hybFat 3138.274 kb on - strand, within hybFat 3138.363 kb on + strandat 3138.474 kb on + strand, within hybEat 3138.474 kb on + strand, within hybE

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol%
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3,135,848 + gss b2988 0.63 +0.1
3,135,848 + gss b2988 0.63 +1.0
3,135,902 - gss b2988 0.65 +1.1
3,135,959 - gss b2988 0.68 -0.5
3,136,065 + gss b2988 0.74 -0.2
3,136,072 + gss b2988 0.75 +0.2
3,136,073 - gss b2988 0.75 +0.9
3,136,245 + gss b2988 0.84 -0.0
3,136,254 - gss b2988 0.84 -0.2
3,136,321 - gss b2988 0.88 -1.1
3,136,325 - gss b2988 0.88 -0.2
3,136,325 - gss b2988 0.88 +1.8
3,136,472 - -0.9
3,136,472 - -1.5
3,136,502 - +1.8
3,136,505 - -1.0
3,136,505 - -0.2
3,136,534 + -1.1
3,136,629 + -0.2
3,136,629 + +0.3
3,136,637 - -0.5
3,136,647 - +0.2
3,136,648 - +1.0
3,136,706 + +0.4
3,136,706 + +0.5
3,136,729 - -1.9
3,137,122 - b2989 b2989 0.43 -0.1
3,137,208 + b2989 b2989 0.53 -1.0
3,137,208 + b2989 b2989 0.53 -1.3
3,137,294 + b2989 b2989 0.63 +2.0
3,137,339 - b2989 b2989 0.68 +0.4
3,137,360 + -0.5
3,137,372 + b2989 b2989 0.72 -1.1
3,137,372 + b2989 b2989 0.72 -0.9
3,137,406 + b2989 b2989 0.76 -1.0
3,137,414 - b2989 b2989 0.77 +0.2
3,137,463 + b2989 b2989 0.82 -1.7
3,137,525 - b2989 b2989 0.90 -2.7
3,137,528 + b2989 b2989 0.90 +1.8
3,137,615 - -0.0
3,137,615 - -0.0
3,137,691 - -1.2
3,137,700 + +1.6
3,137,714 + -2.2
3,137,717 - -1.0
3,137,839 - hybG b2990 0.41 +0.0
3,137,839 - hybG b2990 0.41 -1.1
3,137,907 + hybG b2990 0.68 +1.9
3,137,986 + -1.2
3,137,986 + +0.3
3,138,086 + hybF b2991 0.25 -0.4
3,138,146 - hybF b2991 0.43 -0.7
3,138,152 + hybF b2991 0.45 -1.3
3,138,166 + hybF b2991 0.49 +1.7
3,138,233 - hybF b2991 0.68 +0.4
3,138,274 - hybF b2991 0.80 -1.3
3,138,274 - hybF b2991 0.80 +0.3
3,138,363 + -1.2
3,138,474 + hybE b2992 0.29 -1.0
3,138,474 + hybE b2992 0.29 -1.3

Or see this region's nucleotide sequence