Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gss and b2989 are separated by 204 nucleotides b2989 and hybG are separated by 122 nucleotides hybG and hybF are separated by 12 nucleotides hybF and hybE overlap by 8 nucleotides
b2988: gss - fused glutathionylspermidine amidase/glutathionylspermidine synthetase (NCBI), at 3,134,685 to 3,136,544
gss
b2989: b2989 - orf, hypothetical protein (VIMSS), at 3,136,749 to 3,137,615
b2989
b2990: hybG - hydrogenase 2 accessory protein (NCBI), at 3,137,738 to 3,137,986
hybG
b2991: hybF - protein involved with the maturation of hydrogenases 1 and 2 (NCBI), at 3,137,999 to 3,138,340
hybF
b2992: hybE - hydrogenase 2-specific chaperone (NCBI), at 3,138,333 to 3,138,821
hybE
Position (kb)
3136
3137
3138 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3135.848 kb on + strand, within gss at 3135.848 kb on + strand, within gss at 3135.902 kb on - strand, within gss at 3135.959 kb on - strand, within gss at 3136.065 kb on + strand, within gss at 3136.072 kb on + strand, within gss at 3136.073 kb on - strand, within gss at 3136.245 kb on + strand, within gss at 3136.254 kb on - strand, within gss at 3136.321 kb on - strand, within gss at 3136.325 kb on - strand, within gss at 3136.325 kb on - strand, within gss at 3136.472 kb on - strand at 3136.472 kb on - strand at 3136.502 kb on - strand at 3136.505 kb on - strand at 3136.505 kb on - strand at 3136.534 kb on + strand at 3136.629 kb on + strand at 3136.629 kb on + strand at 3136.637 kb on - strand at 3136.647 kb on - strand at 3136.648 kb on - strand at 3136.706 kb on + strand at 3136.706 kb on + strand at 3136.729 kb on - strand at 3137.122 kb on - strand, within b2989 at 3137.208 kb on + strand, within b2989 at 3137.208 kb on + strand, within b2989 at 3137.294 kb on + strand, within b2989 at 3137.339 kb on - strand, within b2989 at 3137.360 kb on + strand at 3137.372 kb on + strand, within b2989 at 3137.372 kb on + strand, within b2989 at 3137.406 kb on + strand, within b2989 at 3137.414 kb on - strand, within b2989 at 3137.463 kb on + strand, within b2989 at 3137.525 kb on - strand, within b2989 at 3137.528 kb on + strand, within b2989 at 3137.615 kb on - strand at 3137.615 kb on - strand at 3137.691 kb on - strand at 3137.700 kb on + strand at 3137.714 kb on + strand at 3137.717 kb on - strand at 3137.839 kb on - strand, within hybG at 3137.839 kb on - strand, within hybG at 3137.907 kb on + strand, within hybG at 3137.986 kb on + strand at 3137.986 kb on + strand at 3138.086 kb on + strand, within hybF at 3138.146 kb on - strand, within hybF at 3138.152 kb on + strand, within hybF at 3138.166 kb on + strand, within hybF at 3138.233 kb on - strand, within hybF at 3138.274 kb on - strand, within hybF at 3138.274 kb on - strand, within hybF at 3138.363 kb on + strand at 3138.474 kb on + strand, within hybE at 3138.474 kb on + strand, within hybE
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 3,135,848 + gss b2988 0.63 +0.1 3,135,848 + gss b2988 0.63 +1.0 3,135,902 - gss b2988 0.65 +1.1 3,135,959 - gss b2988 0.68 -0.5 3,136,065 + gss b2988 0.74 -0.2 3,136,072 + gss b2988 0.75 +0.2 3,136,073 - gss b2988 0.75 +0.9 3,136,245 + gss b2988 0.84 -0.0 3,136,254 - gss b2988 0.84 -0.2 3,136,321 - gss b2988 0.88 -1.1 3,136,325 - gss b2988 0.88 -0.2 3,136,325 - gss b2988 0.88 +1.8 3,136,472 - -0.9 3,136,472 - -1.5 3,136,502 - +1.8 3,136,505 - -1.0 3,136,505 - -0.2 3,136,534 + -1.1 3,136,629 + -0.2 3,136,629 + +0.3 3,136,637 - -0.5 3,136,647 - +0.2 3,136,648 - +1.0 3,136,706 + +0.4 3,136,706 + +0.5 3,136,729 - -1.9 3,137,122 - b2989 b2989 0.43 -0.1 3,137,208 + b2989 b2989 0.53 -1.0 3,137,208 + b2989 b2989 0.53 -1.3 3,137,294 + b2989 b2989 0.63 +2.0 3,137,339 - b2989 b2989 0.68 +0.4 3,137,360 + -0.5 3,137,372 + b2989 b2989 0.72 -1.1 3,137,372 + b2989 b2989 0.72 -0.9 3,137,406 + b2989 b2989 0.76 -1.0 3,137,414 - b2989 b2989 0.77 +0.2 3,137,463 + b2989 b2989 0.82 -1.7 3,137,525 - b2989 b2989 0.90 -2.7 3,137,528 + b2989 b2989 0.90 +1.8 3,137,615 - -0.0 3,137,615 - -0.0 3,137,691 - -1.2 3,137,700 + +1.6 3,137,714 + -2.2 3,137,717 - -1.0 3,137,839 - hybG b2990 0.41 +0.0 3,137,839 - hybG b2990 0.41 -1.1 3,137,907 + hybG b2990 0.68 +1.9 3,137,986 + -1.2 3,137,986 + +0.3 3,138,086 + hybF b2991 0.25 -0.4 3,138,146 - hybF b2991 0.43 -0.7 3,138,152 + hybF b2991 0.45 -1.3 3,138,166 + hybF b2991 0.49 +1.7 3,138,233 - hybF b2991 0.68 +0.4 3,138,274 - hybF b2991 0.80 -1.3 3,138,274 - hybF b2991 0.80 +0.3 3,138,363 + -1.2 3,138,474 + hybE b2992 0.29 -1.0 3,138,474 + hybE b2992 0.29 -1.3
Or see this region's nucleotide sequence