Strain Fitness in Escherichia coli BW25113 around b2826

Experiment: 3-methyl-1-butanol 1.25 vol%

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-1-butanol 1.25 vol%
2,960,736	+	ppdC	b2823	0.84	+1.4
2,960,759	-				+0.4
2,960,759	-				-1.6
2,960,974	-	ygdB	b2824	0.50	+0.8
2,961,100	+	ygdB	b2824	0.81	-0.3
2,961,100	+	ygdB	b2824	0.81	-0.4
2,961,102	-	ygdB	b2824	0.81	+0.3
2,961,141	+				+0.3
2,961,173	+				-1.3
2,961,343	-	ppdB	b2825	0.30	-1.4
2,961,469	+	ppdB	b2825	0.52	+2.5
2,961,469	+	ppdB	b2825	0.52	+0.8
2,961,483	-	ppdB	b2825	0.55	-1.6
2,961,483	-	ppdB	b2825	0.55	-0.5
2,961,493	-	ppdB	b2825	0.56	+0.5
2,961,493	-	ppdB	b2825	0.56	+1.8
2,961,518	+	ppdB	b2825	0.61	+0.7
2,961,529	-	ppdB	b2825	0.63	-1.1
2,961,529	-	ppdB	b2825	0.63	+2.6
2,961,543	-	ppdB	b2825	0.65	+2.6
2,961,543	-	ppdB	b2825	0.65	-0.2
2,961,543	-	ppdB	b2825	0.65	-3.8
2,961,664	+	ppdB	b2825	0.87	+1.0
2,961,664	+	ppdB	b2825	0.87	-0.5
2,961,794	+	ppdA	b2826	0.14	-2.8
2,961,794	+	ppdA	b2826	0.14	-1.2
2,961,832	-	ppdA	b2826	0.22	-0.3
2,961,832	-	ppdA	b2826	0.22	+1.9
2,961,852	-	ppdA	b2826	0.26	-0.9
2,961,875	+	ppdA	b2826	0.31	+0.6
2,961,875	+	ppdA	b2826	0.31	-0.5
2,961,906	+	ppdA	b2826	0.38	+0.0
2,961,918	+	ppdA	b2826	0.40	-0.2
2,961,943	+	ppdA	b2826	0.45	-0.5
2,961,943	+	ppdA	b2826	0.45	-0.9
2,961,958	+	ppdA	b2826	0.49	-1.0
2,961,958	+	ppdA	b2826	0.49	+0.8
2,961,963	+	ppdA	b2826	0.50	-2.3
2,962,113	+	ppdA	b2826	0.82	+0.4
2,962,122	-	ppdA	b2826	0.83	-1.7
2,962,130	+	ppdA	b2826	0.85	-1.4
2,962,130	+	ppdA	b2826	0.85	-1.9
2,962,149	-	ppdA	b2826	0.89	+0.9
2,962,149	-	ppdA	b2826	0.89	+0.6
2,962,151	+	ppdA	b2826	0.90	+0.2
2,962,173	-				+0.8
2,962,175	+				+0.4
2,962,175	+				-1.1
2,962,175	+				+1.0
2,962,175	+				+1.7
2,962,183	-				+0.4
2,962,215	-				+0.7
2,962,271	+				-1.7
2,962,281	+				-2.0
2,962,281	+				+1.8
2,962,289	-				+1.3
2,962,289	-				-2.4