Strain Fitness in Escherichia coli BW25113 around b2762

Experiment: 3-methyl-1-butanol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygcB and sokX are separated by 134 nucleotidessokX and cysH are separated by 168 nucleotidescysH and cysI are separated by 74 nucleotides b2761: ygcB - conserved protein, member of DEAD box family (NCBI), at 2,882,575 to 2,885,241 ygcB b4701: sokX - sok-related sRNA, function unknown (RefSeq), at 2,885,376 to 2,885,431 sokX b2762: cysH - phosphoadenosine phosphosulfate reductase (NCBI), at 2,885,600 to 2,886,334 cysH b2763: cysI - sulfite reductase, beta subunit, NAD(P)-binding, heme-binding (NCBI), at 2,886,409 to 2,888,121 cysI Position (kb) 2885 2886 2887Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 2884.606 kb on + strand, within ygcBat 2884.606 kb on + strand, within ygcBat 2884.667 kb on + strand, within ygcBat 2884.676 kb on + strand, within ygcBat 2884.698 kb on + strand, within ygcBat 2884.699 kb on - strand, within ygcBat 2884.769 kb on + strand, within ygcBat 2884.770 kb on + strand, within ygcBat 2884.770 kb on + strand, within ygcBat 2884.775 kb on - strand, within ygcBat 2884.778 kb on + strand, within ygcBat 2884.778 kb on + strand, within ygcBat 2884.786 kb on - strand, within ygcBat 2884.786 kb on - strand, within ygcBat 2884.793 kb on + strand, within ygcBat 2884.809 kb on - strand, within ygcBat 2884.809 kb on - strand, within ygcBat 2884.811 kb on - strand, within ygcBat 2884.879 kb on + strand, within ygcBat 2884.879 kb on + strand, within ygcBat 2884.890 kb on + strand, within ygcBat 2884.921 kb on - strand, within ygcBat 2885.142 kb on + strandat 2885.258 kb on - strandat 2885.310 kb on - strandat 2885.310 kb on - strandat 2885.325 kb on + strandat 2885.328 kb on + strandat 2885.328 kb on + strandat 2885.333 kb on - strandat 2885.345 kb on + strandat 2885.357 kb on - strandat 2885.365 kb on + strandat 2885.365 kb on + strandat 2885.390 kb on - strand, within sokXat 2885.415 kb on - strand, within sokXat 2885.443 kb on + strandat 2885.448 kb on - strandat 2885.451 kb on + strandat 2885.451 kb on + strandat 2885.455 kb on + strandat 2885.455 kb on + strandat 2885.456 kb on + strandat 2885.464 kb on - strandat 2885.479 kb on + strandat 2885.479 kb on + strandat 2885.482 kb on + strandat 2885.482 kb on + strandat 2885.484 kb on + strandat 2885.484 kb on + strandat 2885.484 kb on + strandat 2885.573 kb on - strandat 2885.616 kb on - strandat 2885.616 kb on - strandat 2885.616 kb on - strandat 2885.617 kb on - strandat 2885.691 kb on - strand, within cysHat 2885.697 kb on - strand, within cysHat 2885.742 kb on - strand, within cysHat 2885.767 kb on - strand, within cysHat 2885.767 kb on - strand, within cysHat 2885.777 kb on - strand, within cysHat 2885.806 kb on - strand, within cysHat 2885.807 kb on - strand, within cysHat 2885.807 kb on - strand, within cysHat 2885.808 kb on - strand, within cysHat 2885.812 kb on - strand, within cysHat 2885.812 kb on - strand, within cysHat 2885.826 kb on - strand, within cysHat 2885.854 kb on - strand, within cysHat 2885.855 kb on - strand, within cysHat 2885.857 kb on + strand, within cysHat 2885.877 kb on + strand, within cysHat 2885.877 kb on - strand, within cysHat 2885.896 kb on - strand, within cysHat 2885.913 kb on - strand, within cysHat 2885.920 kb on - strand, within cysHat 2885.928 kb on + strand, within cysHat 2885.977 kb on - strand, within cysHat 2885.977 kb on - strand, within cysHat 2886.023 kb on - strand, within cysHat 2886.035 kb on - strandat 2886.141 kb on + strand, within cysHat 2886.157 kb on - strand, within cysHat 2886.451 kb on - strandat 2886.451 kb on - strandat 2886.512 kb on + strandat 2886.520 kb on - strandat 2886.520 kb on - strandat 2886.533 kb on - strandat 2886.663 kb on - strand, within cysIat 2886.687 kb on - strand, within cysIat 2886.687 kb on - strand, within cysIat 2886.780 kb on + strand, within cysIat 2886.780 kb on + strand, within cysIat 2886.806 kb on + strand, within cysIat 2886.881 kb on - strand, within cysIat 2886.881 kb on - strand, within cysIat 2887.065 kb on - strand, within cysIat 2887.110 kb on + strand, within cysIat 2887.115 kb on - strand, within cysIat 2887.115 kb on - strand, within cysIat 2887.203 kb on - strand, within cysIat 2887.203 kb on - strand, within cysIat 2887.234 kb on - strand, within cysI

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol%
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2,884,606 + ygcB b2761 0.76 -0.7
2,884,606 + ygcB b2761 0.76 +0.2
2,884,667 + ygcB b2761 0.78 +2.9
2,884,676 + ygcB b2761 0.79 +0.4
2,884,698 + ygcB b2761 0.80 +0.0
2,884,699 - ygcB b2761 0.80 +0.1
2,884,769 + ygcB b2761 0.82 -1.7
2,884,770 + ygcB b2761 0.82 -1.6
2,884,770 + ygcB b2761 0.82 +0.1
2,884,775 - ygcB b2761 0.82 +0.8
2,884,778 + ygcB b2761 0.83 +0.1
2,884,778 + ygcB b2761 0.83 -0.4
2,884,786 - ygcB b2761 0.83 -1.0
2,884,786 - ygcB b2761 0.83 +0.8
2,884,793 + ygcB b2761 0.83 +0.4
2,884,809 - ygcB b2761 0.84 +1.6
2,884,809 - ygcB b2761 0.84 -0.1
2,884,811 - ygcB b2761 0.84 +0.9
2,884,879 + ygcB b2761 0.86 -0.7
2,884,879 + ygcB b2761 0.86 +1.1
2,884,890 + ygcB b2761 0.87 +0.4
2,884,921 - ygcB b2761 0.88 -1.3
2,885,142 + -1.0
2,885,258 - +2.9
2,885,310 - -2.5
2,885,310 - +0.7
2,885,325 + -0.7
2,885,328 + -0.0
2,885,328 + +0.2
2,885,333 - -0.9
2,885,345 + +0.8
2,885,357 - -0.7
2,885,365 + +1.5
2,885,365 + +0.4
2,885,390 - sokX b4701 0.25 -1.0
2,885,415 - sokX b4701 0.70 +0.9
2,885,443 + -0.6
2,885,448 - -0.2
2,885,451 + +0.5
2,885,451 + -0.8
2,885,455 + +1.5
2,885,455 + +0.2
2,885,456 + +0.6
2,885,464 - +0.9
2,885,479 + -1.0
2,885,479 + -0.2
2,885,482 + -0.1
2,885,482 + +0.0
2,885,484 + +0.6
2,885,484 + +1.3
2,885,484 + -0.2
2,885,573 - +0.2
2,885,616 - +0.9
2,885,616 - -0.4
2,885,616 - +1.1
2,885,617 - -1.8
2,885,691 - cysH b2762 0.12 -0.8
2,885,697 - cysH b2762 0.13 -2.2
2,885,742 - cysH b2762 0.19 -1.5
2,885,767 - cysH b2762 0.23 +0.3
2,885,767 - cysH b2762 0.23 -1.4
2,885,777 - cysH b2762 0.24 +0.4
2,885,806 - cysH b2762 0.28 +0.8
2,885,807 - cysH b2762 0.28 -2.1
2,885,807 - cysH b2762 0.28 +0.1
2,885,808 - cysH b2762 0.28 +0.2
2,885,812 - cysH b2762 0.29 -1.1
2,885,812 - cysH b2762 0.29 -0.1
2,885,826 - cysH b2762 0.31 -2.6
2,885,854 - cysH b2762 0.35 +1.2
2,885,855 - cysH b2762 0.35 +1.0
2,885,857 + cysH b2762 0.35 +1.1
2,885,877 + cysH b2762 0.38 -1.7
2,885,877 - cysH b2762 0.38 -0.9
2,885,896 - cysH b2762 0.40 +0.7
2,885,913 - cysH b2762 0.43 -2.0
2,885,920 - cysH b2762 0.44 -1.1
2,885,928 + cysH b2762 0.45 +0.0
2,885,977 - cysH b2762 0.51 +0.2
2,885,977 - cysH b2762 0.51 -1.4
2,886,023 - cysH b2762 0.58 +1.2
2,886,035 - +0.9
2,886,141 + cysH b2762 0.74 -0.3
2,886,157 - cysH b2762 0.76 +2.3
2,886,451 - +0.4
2,886,451 - +1.7
2,886,512 + +0.3
2,886,520 - +1.8
2,886,520 - -0.3
2,886,533 - -0.7
2,886,663 - cysI b2763 0.15 -0.1
2,886,687 - cysI b2763 0.16 -0.5
2,886,687 - cysI b2763 0.16 -0.2
2,886,780 + cysI b2763 0.22 -0.4
2,886,780 + cysI b2763 0.22 +3.9
2,886,806 + cysI b2763 0.23 -0.7
2,886,881 - cysI b2763 0.28 -0.0
2,886,881 - cysI b2763 0.28 +1.4
2,887,065 - cysI b2763 0.38 -1.1
2,887,110 + cysI b2763 0.41 -2.0
2,887,115 - cysI b2763 0.41 -0.6
2,887,115 - cysI b2763 0.41 -1.7
2,887,203 - cysI b2763 0.46 +0.0
2,887,203 - cysI b2763 0.46 +0.8
2,887,234 - cysI b2763 0.48 +0.2

Or see this region's nucleotide sequence