Strain Fitness in Escherichia coli BW25113 around b2718

Experiment: 3-methyl-1-butanol 1.25 vol%

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntascB and hycI are separated by 158 nucleotideshycI and hycH overlap by 8 nucleotideshycH and hycG overlap by 4 nucleotideshycG and hycF overlap by 1 nucleotides b2716: ascB - cryptic 6-phospho-beta-glucosidase (NCBI), at 2,839,012 to 2,840,436 ascB b2717: hycI - protease involved in processing C-terminal end of HycE (NCBI), at 2,840,595 to 2,841,065 hycI b2718: hycH - protein required for maturation of hydrogenase 3 (NCBI), at 2,841,058 to 2,841,468 hycH b2719: hycG - hydrogenase 3 and formate hydrogenase complex, HycG subunit (NCBI), at 2,841,465 to 2,842,232 hycG b2720: hycF - formate hydrogenlyase complex iron-sulfur protein (NCBI), at 2,842,232 to 2,842,774 hycF Position (kb) 2841 2842Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2840.238 kb on + strand, within ascBat 2840.256 kb on - strand, within ascBat 2840.263 kb on + strand, within ascBat 2840.281 kb on - strand, within ascBat 2840.497 kb on - strandat 2840.551 kb on - strandat 2840.558 kb on + strandat 2840.644 kb on + strand, within hycIat 2840.688 kb on - strand, within hycIat 2840.699 kb on + strand, within hycIat 2840.699 kb on + strand, within hycIat 2840.711 kb on - strand, within hycIat 2840.711 kb on - strand, within hycIat 2840.731 kb on - strand, within hycIat 2840.731 kb on - strand, within hycIat 2841.008 kb on + strand, within hycIat 2841.071 kb on + strandat 2841.119 kb on + strand, within hycHat 2841.150 kb on + strand, within hycHat 2841.167 kb on + strand, within hycHat 2841.175 kb on - strand, within hycHat 2841.207 kb on + strand, within hycHat 2841.225 kb on + strand, within hycHat 2841.272 kb on - strand, within hycHat 2841.300 kb on + strand, within hycHat 2841.300 kb on + strand, within hycHat 2841.327 kb on - strand, within hycHat 2841.327 kb on - strand, within hycHat 2841.338 kb on - strand, within hycHat 2841.384 kb on - strand, within hycHat 2841.572 kb on + strand, within hycGat 2841.581 kb on - strand, within hycGat 2841.622 kb on - strand, within hycGat 2841.645 kb on - strand, within hycGat 2841.648 kb on - strand, within hycGat 2841.713 kb on + strand, within hycGat 2841.774 kb on - strand, within hycGat 2841.821 kb on - strand, within hycGat 2841.861 kb on - strand, within hycGat 2841.870 kb on - strand, within hycGat 2842.019 kb on + strand, within hycGat 2842.047 kb on - strand, within hycGat 2842.093 kb on - strand, within hycGat 2842.093 kb on - strand, within hycGat 2842.113 kb on + strand, within hycGat 2842.245 kb on + strandat 2842.253 kb on - strandat 2842.346 kb on + strand, within hycFat 2842.367 kb on - strand, within hycFat 2842.377 kb on + strand, within hycFat 2842.384 kb on - strand, within hycF

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol%
remove
2,840,238 + ascB b2716 0.86 -0.5
2,840,256 - ascB b2716 0.87 -3.0
2,840,263 + ascB b2716 0.88 -0.4
2,840,281 - ascB b2716 0.89 +2.4
2,840,497 - +0.1
2,840,551 - +0.0
2,840,558 + -1.7
2,840,644 + hycI b2717 0.10 +2.3
2,840,688 - hycI b2717 0.20 -1.4
2,840,699 + hycI b2717 0.22 +1.4
2,840,699 + hycI b2717 0.22 -1.3
2,840,711 - hycI b2717 0.25 -2.6
2,840,711 - hycI b2717 0.25 +0.2
2,840,731 - hycI b2717 0.29 -1.3
2,840,731 - hycI b2717 0.29 +0.2
2,841,008 + hycI b2717 0.88 -0.7
2,841,071 + -1.1
2,841,119 + hycH b2718 0.15 -0.8
2,841,150 + hycH b2718 0.22 -1.2
2,841,167 + hycH b2718 0.27 +0.5
2,841,175 - hycH b2718 0.28 -0.3
2,841,207 + hycH b2718 0.36 -0.1
2,841,225 + hycH b2718 0.41 -0.1
2,841,272 - hycH b2718 0.52 -1.8
2,841,300 + hycH b2718 0.59 -0.1
2,841,300 + hycH b2718 0.59 +0.2
2,841,327 - hycH b2718 0.65 -1.8
2,841,327 - hycH b2718 0.65 -2.9
2,841,338 - hycH b2718 0.68 +3.1
2,841,384 - hycH b2718 0.79 +1.7
2,841,572 + hycG b2719 0.14 -0.1
2,841,581 - hycG b2719 0.15 +1.7
2,841,622 - hycG b2719 0.20 -0.5
2,841,645 - hycG b2719 0.23 +1.1
2,841,648 - hycG b2719 0.24 -2.3
2,841,713 + hycG b2719 0.32 -1.0
2,841,774 - hycG b2719 0.40 -2.1
2,841,821 - hycG b2719 0.46 +2.1
2,841,861 - hycG b2719 0.52 -0.5
2,841,870 - hycG b2719 0.53 -0.4
2,842,019 + hycG b2719 0.72 -0.2
2,842,047 - hycG b2719 0.76 -1.2
2,842,093 - hycG b2719 0.82 +0.5
2,842,093 - hycG b2719 0.82 -0.9
2,842,113 + hycG b2719 0.84 -1.5
2,842,245 + +1.9
2,842,253 - -0.4
2,842,346 + hycF b2720 0.21 +1.9
2,842,367 - hycF b2720 0.25 -1.9
2,842,377 + hycF b2720 0.27 -1.5
2,842,384 - hycF b2720 0.28 +1.2

Or see this region's nucleotide sequence