Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ascB and hycI are separated by 158 nucleotides hycI and hycH overlap by 8 nucleotides hycH and hycG overlap by 4 nucleotides hycG and hycF overlap by 1 nucleotides
b2716: ascB - cryptic 6-phospho-beta-glucosidase (NCBI), at 2,839,012 to 2,840,436
ascB
b2717: hycI - protease involved in processing C-terminal end of HycE (NCBI), at 2,840,595 to 2,841,065
hycI
b2718: hycH - protein required for maturation of hydrogenase 3 (NCBI), at 2,841,058 to 2,841,468
hycH
b2719: hycG - hydrogenase 3 and formate hydrogenase complex, HycG subunit (NCBI), at 2,841,465 to 2,842,232
hycG
b2720: hycF - formate hydrogenlyase complex iron-sulfur protein (NCBI), at 2,842,232 to 2,842,774
hycF
Position (kb)
2841
2842 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2840.238 kb on + strand, within ascB at 2840.256 kb on - strand, within ascB at 2840.263 kb on + strand, within ascB at 2840.281 kb on - strand, within ascB at 2840.497 kb on - strand at 2840.551 kb on - strand at 2840.558 kb on + strand at 2840.644 kb on + strand, within hycI at 2840.688 kb on - strand, within hycI at 2840.699 kb on + strand, within hycI at 2840.699 kb on + strand, within hycI at 2840.711 kb on - strand, within hycI at 2840.711 kb on - strand, within hycI at 2840.731 kb on - strand, within hycI at 2840.731 kb on - strand, within hycI at 2841.008 kb on + strand, within hycI at 2841.071 kb on + strand at 2841.119 kb on + strand, within hycH at 2841.150 kb on + strand, within hycH at 2841.167 kb on + strand, within hycH at 2841.175 kb on - strand, within hycH at 2841.207 kb on + strand, within hycH at 2841.225 kb on + strand, within hycH at 2841.272 kb on - strand, within hycH at 2841.300 kb on + strand, within hycH at 2841.300 kb on + strand, within hycH at 2841.327 kb on - strand, within hycH at 2841.327 kb on - strand, within hycH at 2841.338 kb on - strand, within hycH at 2841.384 kb on - strand, within hycH at 2841.572 kb on + strand, within hycG at 2841.581 kb on - strand, within hycG at 2841.622 kb on - strand, within hycG at 2841.645 kb on - strand, within hycG at 2841.648 kb on - strand, within hycG at 2841.713 kb on + strand, within hycG at 2841.774 kb on - strand, within hycG at 2841.821 kb on - strand, within hycG at 2841.861 kb on - strand, within hycG at 2841.870 kb on - strand, within hycG at 2842.019 kb on + strand, within hycG at 2842.047 kb on - strand, within hycG at 2842.093 kb on - strand, within hycG at 2842.093 kb on - strand, within hycG at 2842.113 kb on + strand, within hycG at 2842.245 kb on + strand at 2842.253 kb on - strand at 2842.346 kb on + strand, within hycF at 2842.367 kb on - strand, within hycF at 2842.377 kb on + strand, within hycF at 2842.384 kb on - strand, within hycF
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 2,840,238 + ascB b2716 0.86 -0.5 2,840,256 - ascB b2716 0.87 -3.0 2,840,263 + ascB b2716 0.88 -0.4 2,840,281 - ascB b2716 0.89 +2.4 2,840,497 - +0.1 2,840,551 - +0.0 2,840,558 + -1.7 2,840,644 + hycI b2717 0.10 +2.3 2,840,688 - hycI b2717 0.20 -1.4 2,840,699 + hycI b2717 0.22 +1.4 2,840,699 + hycI b2717 0.22 -1.3 2,840,711 - hycI b2717 0.25 -2.6 2,840,711 - hycI b2717 0.25 +0.2 2,840,731 - hycI b2717 0.29 -1.3 2,840,731 - hycI b2717 0.29 +0.2 2,841,008 + hycI b2717 0.88 -0.7 2,841,071 + -1.1 2,841,119 + hycH b2718 0.15 -0.8 2,841,150 + hycH b2718 0.22 -1.2 2,841,167 + hycH b2718 0.27 +0.5 2,841,175 - hycH b2718 0.28 -0.3 2,841,207 + hycH b2718 0.36 -0.1 2,841,225 + hycH b2718 0.41 -0.1 2,841,272 - hycH b2718 0.52 -1.8 2,841,300 + hycH b2718 0.59 -0.1 2,841,300 + hycH b2718 0.59 +0.2 2,841,327 - hycH b2718 0.65 -1.8 2,841,327 - hycH b2718 0.65 -2.9 2,841,338 - hycH b2718 0.68 +3.1 2,841,384 - hycH b2718 0.79 +1.7 2,841,572 + hycG b2719 0.14 -0.1 2,841,581 - hycG b2719 0.15 +1.7 2,841,622 - hycG b2719 0.20 -0.5 2,841,645 - hycG b2719 0.23 +1.1 2,841,648 - hycG b2719 0.24 -2.3 2,841,713 + hycG b2719 0.32 -1.0 2,841,774 - hycG b2719 0.40 -2.1 2,841,821 - hycG b2719 0.46 +2.1 2,841,861 - hycG b2719 0.52 -0.5 2,841,870 - hycG b2719 0.53 -0.4 2,842,019 + hycG b2719 0.72 -0.2 2,842,047 - hycG b2719 0.76 -1.2 2,842,093 - hycG b2719 0.82 +0.5 2,842,093 - hycG b2719 0.82 -0.9 2,842,113 + hycG b2719 0.84 -1.5 2,842,245 + +1.9 2,842,253 - -0.4 2,842,346 + hycF b2720 0.21 +1.9 2,842,367 - hycF b2720 0.25 -1.9 2,842,377 + hycF b2720 0.27 -1.5 2,842,384 - hycF b2720 0.28 +1.2
Or see this region's nucleotide sequence