Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ascF and ascB are separated by 8 nucleotides ascB and hycI are separated by 158 nucleotides hycI and hycH overlap by 8 nucleotides
b2715: ascF - fused cellobiose/arbutin/salicin-specific PTS enzymes: IIB component/IC component (NCBI), at 2,837,546 to 2,839,003
ascF
b2716: ascB - cryptic 6-phospho-beta-glucosidase (NCBI), at 2,839,012 to 2,840,436
ascB
b2717: hycI - protease involved in processing C-terminal end of HycE (NCBI), at 2,840,595 to 2,841,065
hycI
b2718: hycH - protein required for maturation of hydrogenase 3 (NCBI), at 2,841,058 to 2,841,468
hycH
Position (kb)
2839
2840
2841 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2838.101 kb on + strand, within ascF at 2838.101 kb on + strand, within ascF at 2838.120 kb on + strand, within ascF at 2838.188 kb on + strand, within ascF at 2838.188 kb on + strand, within ascF at 2838.238 kb on + strand, within ascF at 2838.238 kb on + strand, within ascF at 2838.264 kb on + strand, within ascF at 2838.272 kb on - strand, within ascF at 2838.336 kb on - strand, within ascF at 2838.388 kb on + strand, within ascF at 2838.388 kb on + strand, within ascF at 2838.396 kb on - strand, within ascF at 2838.398 kb on + strand, within ascF at 2838.533 kb on + strand, within ascF at 2838.533 kb on + strand, within ascF at 2838.676 kb on + strand, within ascF at 2838.676 kb on + strand, within ascF at 2838.786 kb on - strand, within ascF at 2838.786 kb on - strand, within ascF at 2838.879 kb on - strand at 2839.120 kb on - strand at 2839.120 kb on - strand at 2839.234 kb on - strand, within ascB at 2839.234 kb on - strand, within ascB at 2839.385 kb on + strand, within ascB at 2839.387 kb on + strand, within ascB at 2839.407 kb on + strand, within ascB at 2839.407 kb on + strand, within ascB at 2839.427 kb on + strand, within ascB at 2839.514 kb on + strand, within ascB at 2839.520 kb on + strand, within ascB at 2839.556 kb on + strand, within ascB at 2839.607 kb on + strand, within ascB at 2839.661 kb on - strand, within ascB at 2839.893 kb on - strand, within ascB at 2839.893 kb on - strand, within ascB at 2840.238 kb on + strand, within ascB at 2840.256 kb on - strand, within ascB at 2840.263 kb on + strand, within ascB at 2840.281 kb on - strand, within ascB at 2840.497 kb on - strand at 2840.551 kb on - strand at 2840.558 kb on + strand at 2840.644 kb on + strand, within hycI at 2840.688 kb on - strand, within hycI at 2840.699 kb on + strand, within hycI at 2840.699 kb on + strand, within hycI at 2840.711 kb on - strand, within hycI at 2840.711 kb on - strand, within hycI at 2840.731 kb on - strand, within hycI at 2840.731 kb on - strand, within hycI at 2841.008 kb on + strand, within hycI at 2841.071 kb on + strand at 2841.119 kb on + strand, within hycH at 2841.150 kb on + strand, within hycH at 2841.167 kb on + strand, within hycH at 2841.175 kb on - strand, within hycH at 2841.207 kb on + strand, within hycH at 2841.225 kb on + strand, within hycH at 2841.272 kb on - strand, within hycH at 2841.300 kb on + strand, within hycH at 2841.300 kb on + strand, within hycH at 2841.327 kb on - strand, within hycH at 2841.327 kb on - strand, within hycH at 2841.338 kb on - strand, within hycH at 2841.384 kb on - strand, within hycH
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 2,838,101 + ascF b2715 0.38 +0.4 2,838,101 + ascF b2715 0.38 -1.7 2,838,120 + ascF b2715 0.39 +0.7 2,838,188 + ascF b2715 0.44 -3.0 2,838,188 + ascF b2715 0.44 -0.6 2,838,238 + ascF b2715 0.47 -0.2 2,838,238 + ascF b2715 0.47 -1.8 2,838,264 + ascF b2715 0.49 +1.5 2,838,272 - ascF b2715 0.50 -1.4 2,838,336 - ascF b2715 0.54 -0.6 2,838,388 + ascF b2715 0.58 -0.3 2,838,388 + ascF b2715 0.58 +1.6 2,838,396 - ascF b2715 0.58 -0.7 2,838,398 + ascF b2715 0.58 +1.5 2,838,533 + ascF b2715 0.68 -0.2 2,838,533 + ascF b2715 0.68 -0.4 2,838,676 + ascF b2715 0.78 +2.2 2,838,676 + ascF b2715 0.78 +1.0 2,838,786 - ascF b2715 0.85 +0.6 2,838,786 - ascF b2715 0.85 -2.1 2,838,879 - +1.2 2,839,120 - +0.6 2,839,120 - -2.2 2,839,234 - ascB b2716 0.16 +0.8 2,839,234 - ascB b2716 0.16 -1.9 2,839,385 + ascB b2716 0.26 -1.7 2,839,387 + ascB b2716 0.26 -0.6 2,839,407 + ascB b2716 0.28 +1.9 2,839,407 + ascB b2716 0.28 -1.7 2,839,427 + ascB b2716 0.29 -3.7 2,839,514 + ascB b2716 0.35 -1.4 2,839,520 + ascB b2716 0.36 -1.1 2,839,556 + ascB b2716 0.38 -2.0 2,839,607 + ascB b2716 0.42 -0.1 2,839,661 - ascB b2716 0.46 -0.0 2,839,893 - ascB b2716 0.62 -1.6 2,839,893 - ascB b2716 0.62 +0.3 2,840,238 + ascB b2716 0.86 -0.5 2,840,256 - ascB b2716 0.87 -3.0 2,840,263 + ascB b2716 0.88 -0.4 2,840,281 - ascB b2716 0.89 +2.4 2,840,497 - +0.1 2,840,551 - +0.0 2,840,558 + -1.7 2,840,644 + hycI b2717 0.10 +2.3 2,840,688 - hycI b2717 0.20 -1.4 2,840,699 + hycI b2717 0.22 +1.4 2,840,699 + hycI b2717 0.22 -1.3 2,840,711 - hycI b2717 0.25 -2.6 2,840,711 - hycI b2717 0.25 +0.2 2,840,731 - hycI b2717 0.29 -1.3 2,840,731 - hycI b2717 0.29 +0.2 2,841,008 + hycI b2717 0.88 -0.7 2,841,071 + -1.1 2,841,119 + hycH b2718 0.15 -0.8 2,841,150 + hycH b2718 0.22 -1.2 2,841,167 + hycH b2718 0.27 +0.5 2,841,175 - hycH b2718 0.28 -0.3 2,841,207 + hycH b2718 0.36 -0.1 2,841,225 + hycH b2718 0.41 -0.1 2,841,272 - hycH b2718 0.52 -1.8 2,841,300 + hycH b2718 0.59 -0.1 2,841,300 + hycH b2718 0.59 +0.2 2,841,327 - hycH b2718 0.65 -1.8 2,841,327 - hycH b2718 0.65 -2.9 2,841,338 - hycH b2718 0.68 +3.1 2,841,384 - hycH b2718 0.79 +1.7
Or see this region's nucleotide sequence