Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyfB and hyfC are separated by 10 nucleotides hyfC and hyfD are separated by 16 nucleotides
b2482: hyfB - NADH dehydrogenase subunit N (NCBI), at 2,599,840 to 2,601,858
hyfB
b2483: hyfC - hydrogenase 4 membrane subunit (VIMSS), at 2,601,869 to 2,602,816
hyfC
b2484: hyfD - hydrogenase 4 membrane subunit (NCBI), at 2,602,833 to 2,604,272
hyfD
Position (kb)
2601
2602
2603 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2600.889 kb on + strand, within hyfB at 2601.194 kb on + strand, within hyfB at 2601.202 kb on - strand, within hyfB at 2601.245 kb on - strand, within hyfB at 2601.245 kb on - strand, within hyfB at 2601.404 kb on - strand, within hyfB at 2601.421 kb on + strand, within hyfB at 2601.434 kb on + strand, within hyfB at 2601.588 kb on + strand, within hyfB at 2601.644 kb on + strand, within hyfB at 2601.644 kb on + strand, within hyfB at 2601.652 kb on - strand, within hyfB at 2601.652 kb on - strand, within hyfB at 2601.701 kb on - strand at 2601.701 kb on - strand at 2601.810 kb on + strand at 2601.925 kb on + strand at 2601.925 kb on + strand at 2601.988 kb on - strand, within hyfC at 2602.127 kb on + strand, within hyfC at 2602.127 kb on + strand, within hyfC at 2602.150 kb on - strand, within hyfC at 2602.199 kb on - strand, within hyfC at 2602.199 kb on - strand, within hyfC at 2602.325 kb on + strand, within hyfC at 2602.350 kb on + strand, within hyfC at 2602.379 kb on + strand, within hyfC at 2602.382 kb on - strand, within hyfC at 2602.432 kb on - strand, within hyfC at 2602.620 kb on - strand, within hyfC at 2602.620 kb on - strand, within hyfC at 2602.813 kb on + strand at 2602.813 kb on + strand at 2603.063 kb on - strand, within hyfD at 2603.095 kb on - strand, within hyfD at 2603.151 kb on + strand, within hyfD at 2603.151 kb on + strand, within hyfD at 2603.293 kb on + strand, within hyfD at 2603.301 kb on - strand, within hyfD at 2603.328 kb on - strand, within hyfD at 2603.369 kb on + strand, within hyfD at 2603.430 kb on + strand, within hyfD at 2603.464 kb on - strand, within hyfD at 2603.464 kb on - strand, within hyfD at 2603.477 kb on - strand, within hyfD at 2603.547 kb on - strand, within hyfD at 2603.570 kb on - strand, within hyfD at 2603.574 kb on + strand, within hyfD at 2603.574 kb on + strand, within hyfD at 2603.613 kb on + strand, within hyfD at 2603.716 kb on + strand, within hyfD at 2603.726 kb on + strand, within hyfD at 2603.726 kb on + strand, within hyfD at 2603.783 kb on + strand, within hyfD
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 2,600,889 + hyfB b2482 0.52 -0.1 2,601,194 + hyfB b2482 0.67 +1.2 2,601,202 - hyfB b2482 0.67 +0.7 2,601,245 - hyfB b2482 0.70 -0.6 2,601,245 - hyfB b2482 0.70 +1.2 2,601,404 - hyfB b2482 0.77 +2.6 2,601,421 + hyfB b2482 0.78 -0.5 2,601,434 + hyfB b2482 0.79 +0.2 2,601,588 + hyfB b2482 0.87 +0.9 2,601,644 + hyfB b2482 0.89 -1.1 2,601,644 + hyfB b2482 0.89 -1.5 2,601,652 - hyfB b2482 0.90 +0.7 2,601,652 - hyfB b2482 0.90 -1.6 2,601,701 - -1.6 2,601,701 - +0.2 2,601,810 + +0.5 2,601,925 + -2.6 2,601,925 + +2.2 2,601,988 - hyfC b2483 0.13 -0.3 2,602,127 + hyfC b2483 0.27 +1.3 2,602,127 + hyfC b2483 0.27 -0.3 2,602,150 - hyfC b2483 0.30 -0.2 2,602,199 - hyfC b2483 0.35 -2.4 2,602,199 - hyfC b2483 0.35 -0.6 2,602,325 + hyfC b2483 0.48 -2.1 2,602,350 + hyfC b2483 0.51 -0.7 2,602,379 + hyfC b2483 0.54 -1.9 2,602,382 - hyfC b2483 0.54 -0.7 2,602,432 - hyfC b2483 0.59 -1.8 2,602,620 - hyfC b2483 0.79 -2.5 2,602,620 - hyfC b2483 0.79 +2.0 2,602,813 + +0.9 2,602,813 + -2.4 2,603,063 - hyfD b2484 0.16 -0.6 2,603,095 - hyfD b2484 0.18 +0.7 2,603,151 + hyfD b2484 0.22 -0.2 2,603,151 + hyfD b2484 0.22 -0.5 2,603,293 + hyfD b2484 0.32 +1.1 2,603,301 - hyfD b2484 0.33 -3.7 2,603,328 - hyfD b2484 0.34 +1.5 2,603,369 + hyfD b2484 0.37 -0.6 2,603,430 + hyfD b2484 0.41 +0.9 2,603,464 - hyfD b2484 0.44 -1.0 2,603,464 - hyfD b2484 0.44 +1.0 2,603,477 - hyfD b2484 0.45 +0.7 2,603,547 - hyfD b2484 0.50 +0.9 2,603,570 - hyfD b2484 0.51 +0.4 2,603,574 + hyfD b2484 0.51 +1.4 2,603,574 + hyfD b2484 0.51 -0.4 2,603,613 + hyfD b2484 0.54 +0.3 2,603,716 + hyfD b2484 0.61 +0.4 2,603,726 + hyfD b2484 0.62 +1.6 2,603,726 + hyfD b2484 0.62 -2.1 2,603,783 + hyfD b2484 0.66 +1.5
Or see this region's nucleotide sequence