Strain Fitness in Escherichia coli BW25113 around b2480

Experiment: 3-methyl-1-butanol 1.25 vol%

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-1-butanol 1.25 vol%
2,597,909	+				-2.4
2,597,917	-				+1.2
2,597,917	-				+0.3
2,597,962	-				+1.6
2,597,988	-	gcvR	b2479	0.10	+0.4
2,598,033	+	gcvR	b2479	0.18	+0.9
2,598,054	+	gcvR	b2479	0.22	+1.7
2,598,128	-	gcvR	b2479	0.35	+3.7
2,598,129	-	gcvR	b2479	0.35	-0.4
2,598,129	-	gcvR	b2479	0.35	+0.3
2,598,132	+	gcvR	b2479	0.36	+0.0
2,598,132	+	gcvR	b2479	0.36	+2.4
2,598,139	-	gcvR	b2479	0.37	+0.8
2,598,142	+	gcvR	b2479	0.37	+1.9
2,598,212	+	gcvR	b2479	0.50	-0.1
2,598,282	+	gcvR	b2479	0.62	+0.8
2,598,285	+	gcvR	b2479	0.62	+0.3
2,598,303	-	gcvR	b2479	0.65	-1.4
2,598,303	-	gcvR	b2479	0.65	+1.9
2,598,346	-	gcvR	b2479	0.73	-0.8
2,598,400	-	gcvR	b2479	0.82	+1.0
2,598,400	-	gcvR	b2479	0.82	+0.3
2,598,418	-	gcvR	b2479	0.86	+2.2
2,598,427	-	gcvR	b2479	0.87	-0.7
2,598,513	+				+0.2
2,598,621	+	bcp	b2480	0.26	-2.8
2,598,621	+	bcp	b2480	0.26	-0.6
2,598,628	-	bcp	b2480	0.27	+0.4
2,598,639	-	bcp	b2480	0.30	-1.9
2,598,644	-	bcp	b2480	0.31	-1.2
2,598,714	-	bcp	b2480	0.45	+0.1
2,598,757	+	bcp	b2480	0.55	+0.7
2,598,792	-	bcp	b2480	0.62	-1.5
2,598,792	-	bcp	b2480	0.62	-1.6
2,598,938	+				-2.6
2,598,967	-				-3.2
2,598,976	-				+0.6
2,599,005	+				+0.3
2,599,230	+				-0.2
2,599,238	-				-1.3
2,599,319	-	hyfA	b2481	0.16	+0.4
2,599,319	-	hyfA	b2481	0.16	-1.8
2,599,362	+	hyfA	b2481	0.22	-0.6
2,599,370	-	hyfA	b2481	0.24	-2.3
2,599,426	+	hyfA	b2481	0.33	-0.2
2,599,488	-	hyfA	b2481	0.43	-0.8
2,599,528	-	hyfA	b2481	0.49	-0.1
2,599,913	-				-1.4