Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfeR and yfeH are separated by 89 nucleotides yfeH and ypeB overlap by 4 nucleotides ypeB and ligA are separated by 1 nucleotides
b2409: yfeR - predicted DNA-binding transcriptional regulator (NCBI), at 2,523,952 to 2,524,878
yfeR
b2410: yfeH - predicted inner membrane protein (NCBI), at 2,524,968 to 2,525,966
yfeH
b4546: ypeB - hypothetical protein (NCBI), at 2,525,963 to 2,526,181
ypeB
b2411: ligA - NAD-dependent DNA ligase LigA (NCBI), at 2,526,183 to 2,528,198
ligA
Position (kb)
2524
2525
2526 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2523.972 kb on - strand at 2524.005 kb on + strand at 2524.006 kb on + strand at 2524.006 kb on + strand at 2524.231 kb on - strand, within yfeR at 2524.422 kb on + strand, within yfeR at 2524.481 kb on - strand, within yfeR at 2524.614 kb on + strand, within yfeR at 2524.636 kb on - strand, within yfeR at 2524.636 kb on - strand, within yfeR at 2524.653 kb on - strand, within yfeR at 2524.653 kb on - strand, within yfeR at 2524.788 kb on + strand at 2524.794 kb on + strand at 2524.794 kb on + strand at 2524.802 kb on - strand at 2524.941 kb on - strand at 2524.941 kb on - strand at 2525.116 kb on + strand, within yfeH at 2525.248 kb on - strand, within yfeH at 2525.454 kb on - strand, within yfeH at 2525.454 kb on - strand, within yfeH at 2525.454 kb on - strand, within yfeH at 2525.454 kb on - strand, within yfeH at 2525.613 kb on + strand, within yfeH at 2525.709 kb on + strand, within yfeH at 2525.727 kb on - strand, within yfeH at 2525.727 kb on - strand, within yfeH at 2525.887 kb on + strand at 2525.942 kb on + strand at 2526.092 kb on + strand, within ypeB
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 2,523,972 - -0.3 2,524,005 + -2.8 2,524,006 + +1.3 2,524,006 + +0.1 2,524,231 - yfeR b2409 0.30 -0.3 2,524,422 + yfeR b2409 0.51 +1.2 2,524,481 - yfeR b2409 0.57 +0.3 2,524,614 + yfeR b2409 0.71 -0.9 2,524,636 - yfeR b2409 0.74 +0.1 2,524,636 - yfeR b2409 0.74 -1.3 2,524,653 - yfeR b2409 0.76 +1.6 2,524,653 - yfeR b2409 0.76 +0.9 2,524,788 + +0.4 2,524,794 + +1.9 2,524,794 + +0.0 2,524,802 - +1.3 2,524,941 - -0.3 2,524,941 - -2.6 2,525,116 + yfeH b2410 0.15 -1.1 2,525,248 - yfeH b2410 0.28 +0.2 2,525,454 - yfeH b2410 0.49 +2.5 2,525,454 - yfeH b2410 0.49 +1.6 2,525,454 - yfeH b2410 0.49 +3.8 2,525,454 - yfeH b2410 0.49 +1.0 2,525,613 + yfeH b2410 0.65 +0.1 2,525,709 + yfeH b2410 0.74 +2.5 2,525,727 - yfeH b2410 0.76 -1.1 2,525,727 - yfeH b2410 0.76 +1.2 2,525,887 + +0.8 2,525,942 + -1.1 2,526,092 + ypeB b4546 0.59 -1.1
Or see this region's nucleotide sequence