Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfdK and yfdL are separated by 26 nucleotides yfdL and yfdM overlap by 5 nucleotides yfdM and yfdN overlap by 1 nucleotides yfdN and oweS overlap by 4 nucleotides
b2354: yfdK - CPS-53 (KpLE1) prophage; conserved protein (NCBI), at 2,469,099 to 2,469,539
yfdK
b2355: yfdL - CPS-53 (KpLE1) prophage; conserved protein (RefSeq), at 2,469,566 to 2,470,144
yfdL
b2356: yfdM - orf, hypothetical protein (VIMSS), at 2,470,140 to 2,470,409
yfdM
b2357: yfdN - CPS-53 (KpLE1) prophage; predicted protein (NCBI), at 2,470,409 to 2,470,903
yfdN
b2358: oweS - CPS-53 (KpLE1) prophage; predicted defective phage replication protein O (RefSeq), at 2,470,900 to 2,471,607
oweS
Position (kb)
2470
2471 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2469.164 kb on + strand, within yfdK at 2469.164 kb on + strand, within yfdK at 2469.164 kb on + strand, within yfdK at 2469.164 kb on + strand, within yfdK at 2469.164 kb on + strand, within yfdK at 2469.164 kb on + strand, within yfdK at 2469.164 kb on + strand, within yfdK at 2469.164 kb on + strand, within yfdK at 2469.170 kb on - strand, within yfdK at 2469.170 kb on - strand, within yfdK at 2469.172 kb on - strand, within yfdK at 2469.179 kb on + strand, within yfdK at 2469.210 kb on + strand, within yfdK at 2469.210 kb on + strand, within yfdK at 2469.236 kb on - strand, within yfdK at 2469.241 kb on - strand, within yfdK at 2469.331 kb on - strand, within yfdK at 2469.331 kb on - strand, within yfdK at 2469.331 kb on - strand, within yfdK at 2469.332 kb on - strand, within yfdK at 2469.332 kb on - strand, within yfdK at 2469.332 kb on - strand, within yfdK at 2469.332 kb on - strand, within yfdK at 2469.354 kb on - strand, within yfdK at 2469.442 kb on - strand, within yfdK at 2469.442 kb on - strand, within yfdK at 2469.451 kb on - strand, within yfdK at 2469.457 kb on + strand, within yfdK at 2469.457 kb on + strand, within yfdK at 2469.459 kb on + strand, within yfdK at 2469.460 kb on - strand, within yfdK at 2469.461 kb on - strand, within yfdK at 2469.461 kb on - strand, within yfdK at 2469.463 kb on + strand, within yfdK at 2469.596 kb on + strand at 2469.596 kb on + strand at 2469.610 kb on - strand at 2469.626 kb on - strand, within yfdL at 2469.659 kb on - strand, within yfdL at 2469.663 kb on - strand, within yfdL at 2469.663 kb on - strand, within yfdL at 2469.685 kb on + strand, within yfdL at 2469.688 kb on - strand, within yfdL at 2469.695 kb on - strand, within yfdL at 2469.751 kb on - strand, within yfdL at 2469.754 kb on - strand, within yfdL at 2469.758 kb on - strand, within yfdL at 2469.763 kb on - strand, within yfdL at 2469.763 kb on - strand, within yfdL at 2469.763 kb on - strand, within yfdL at 2469.763 kb on - strand, within yfdL at 2469.763 kb on - strand, within yfdL at 2469.763 kb on - strand, within yfdL at 2469.772 kb on + strand, within yfdL at 2469.801 kb on - strand, within yfdL at 2469.852 kb on - strand, within yfdL at 2469.852 kb on - strand, within yfdL at 2469.857 kb on + strand, within yfdL at 2469.865 kb on + strand, within yfdL at 2469.867 kb on - strand, within yfdL at 2469.890 kb on - strand, within yfdL at 2469.940 kb on - strand, within yfdL at 2469.942 kb on - strand, within yfdL at 2469.942 kb on - strand, within yfdL at 2469.998 kb on + strand, within yfdL at 2470.038 kb on - strand, within yfdL at 2470.038 kb on - strand, within yfdL at 2470.049 kb on - strand, within yfdL at 2470.049 kb on - strand, within yfdL at 2470.090 kb on - strand at 2470.168 kb on + strand, within yfdM at 2470.211 kb on - strand, within yfdM at 2470.448 kb on + strand at 2470.448 kb on + strand at 2470.477 kb on + strand, within yfdN at 2470.477 kb on + strand, within yfdN at 2470.484 kb on - strand, within yfdN at 2470.484 kb on - strand, within yfdN at 2470.741 kb on - strand, within yfdN at 2470.913 kb on - strand at 2470.928 kb on + strand at 2471.021 kb on - strand, within oweS at 2471.021 kb on - strand, within oweS at 2471.090 kb on + strand, within oweS at 2471.133 kb on + strand, within oweS at 2471.133 kb on + strand, within oweS at 2471.201 kb on + strand, within oweS at 2471.201 kb on + strand, within oweS at 2471.263 kb on + strand, within oweS at 2471.277 kb on - strand, within oweS at 2471.277 kb on - strand, within oweS at 2471.372 kb on + strand, within oweS at 2471.372 kb on + strand, within oweS
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 2,469,164 + yfdK b2354 0.15 +1.2 2,469,164 + yfdK b2354 0.15 +0.9 2,469,164 + yfdK b2354 0.15 -0.0 2,469,164 + yfdK b2354 0.15 -0.6 2,469,164 + yfdK b2354 0.15 +1.3 2,469,164 + yfdK b2354 0.15 +1.7 2,469,164 + yfdK b2354 0.15 +0.3 2,469,164 + yfdK b2354 0.15 +3.3 2,469,170 - yfdK b2354 0.16 +1.2 2,469,170 - yfdK b2354 0.16 -0.1 2,469,172 - yfdK b2354 0.17 -1.8 2,469,179 + yfdK b2354 0.18 -1.8 2,469,210 + yfdK b2354 0.25 -0.9 2,469,210 + yfdK b2354 0.25 -0.5 2,469,236 - yfdK b2354 0.31 -0.5 2,469,241 - yfdK b2354 0.32 +0.6 2,469,331 - yfdK b2354 0.53 -2.4 2,469,331 - yfdK b2354 0.53 +1.9 2,469,331 - yfdK b2354 0.53 -0.4 2,469,332 - yfdK b2354 0.53 +0.1 2,469,332 - yfdK b2354 0.53 +1.4 2,469,332 - yfdK b2354 0.53 +2.0 2,469,332 - yfdK b2354 0.53 +1.5 2,469,354 - yfdK b2354 0.58 +1.4 2,469,442 - yfdK b2354 0.78 -0.3 2,469,442 - yfdK b2354 0.78 +0.5 2,469,451 - yfdK b2354 0.80 +0.2 2,469,457 + yfdK b2354 0.81 +0.3 2,469,457 + yfdK b2354 0.81 -0.7 2,469,459 + yfdK b2354 0.82 -0.3 2,469,460 - yfdK b2354 0.82 +0.9 2,469,461 - yfdK b2354 0.82 +1.5 2,469,461 - yfdK b2354 0.82 -1.1 2,469,463 + yfdK b2354 0.83 -0.6 2,469,596 + +0.5 2,469,596 + -1.5 2,469,610 - -1.6 2,469,626 - yfdL b2355 0.10 +1.4 2,469,659 - yfdL b2355 0.16 -1.0 2,469,663 - yfdL b2355 0.17 -0.4 2,469,663 - yfdL b2355 0.17 +0.2 2,469,685 + yfdL b2355 0.21 +2.1 2,469,688 - yfdL b2355 0.21 -0.0 2,469,695 - yfdL b2355 0.22 -0.3 2,469,751 - yfdL b2355 0.32 +1.2 2,469,754 - yfdL b2355 0.32 -0.2 2,469,758 - yfdL b2355 0.33 -1.8 2,469,763 - yfdL b2355 0.34 +0.3 2,469,763 - yfdL b2355 0.34 -0.3 2,469,763 - yfdL b2355 0.34 +1.1 2,469,763 - yfdL b2355 0.34 +1.1 2,469,763 - yfdL b2355 0.34 +0.4 2,469,763 - yfdL b2355 0.34 +0.4 2,469,772 + yfdL b2355 0.36 -2.8 2,469,801 - yfdL b2355 0.41 -1.6 2,469,852 - yfdL b2355 0.49 -0.7 2,469,852 - yfdL b2355 0.49 -0.1 2,469,857 + yfdL b2355 0.50 -2.4 2,469,865 + yfdL b2355 0.52 +0.4 2,469,867 - yfdL b2355 0.52 +1.1 2,469,890 - yfdL b2355 0.56 -0.6 2,469,940 - yfdL b2355 0.65 -1.2 2,469,942 - yfdL b2355 0.65 -0.2 2,469,942 - yfdL b2355 0.65 +1.3 2,469,998 + yfdL b2355 0.75 +0.4 2,470,038 - yfdL b2355 0.82 +0.1 2,470,038 - yfdL b2355 0.82 +1.0 2,470,049 - yfdL b2355 0.83 -1.2 2,470,049 - yfdL b2355 0.83 -2.8 2,470,090 - +1.0 2,470,168 + yfdM b2356 0.10 +1.5 2,470,211 - yfdM b2356 0.26 +1.4 2,470,448 + -0.7 2,470,448 + -1.3 2,470,477 + yfdN b2357 0.14 +0.1 2,470,477 + yfdN b2357 0.14 -1.9 2,470,484 - yfdN b2357 0.15 +0.2 2,470,484 - yfdN b2357 0.15 -0.7 2,470,741 - yfdN b2357 0.67 -0.8 2,470,913 - -0.2 2,470,928 + +1.4 2,471,021 - oweS b2358 0.17 -0.1 2,471,021 - oweS b2358 0.17 -1.6 2,471,090 + oweS b2358 0.27 +0.5 2,471,133 + oweS b2358 0.33 -2.7 2,471,133 + oweS b2358 0.33 -0.8 2,471,201 + oweS b2358 0.43 +1.0 2,471,201 + oweS b2358 0.43 +1.4 2,471,263 + oweS b2358 0.51 +1.5 2,471,277 - oweS b2358 0.53 +1.1 2,471,277 - oweS b2358 0.53 +3.5 2,471,372 + oweS b2358 0.67 -0.0 2,471,372 + oweS b2358 0.67 +0.9
Or see this region's nucleotide sequence