Strain Fitness in Escherichia coli BW25113 around b2130

Experiment: 3-methyl-1-butanol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyehW and yehX are separated by 4 nucleotidesyehX and yehY overlap by 8 nucleotidesyehY and yehZ are separated by 6 nucleotides b2128: yehW - predicted transporter subunit: membrane component of ABC superfamily (NCBI), at 2,213,767 to 2,214,498 yehW b2129: yehX - predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 2,214,503 to 2,215,429 yehX b2130: yehY - predicted transporter subunit: membrane component of ABC superfamily (NCBI), at 2,215,422 to 2,216,579 yehY b2131: yehZ - predicted transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 2,216,586 to 2,217,503 yehZ Position (kb) 2215 2216 2217Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2214.491 kb on + strandat 2214.491 kb on + strandat 2214.540 kb on + strandat 2214.540 kb on + strandat 2214.554 kb on - strandat 2214.568 kb on - strandat 2214.700 kb on + strand, within yehXat 2214.708 kb on - strand, within yehXat 2214.965 kb on + strand, within yehXat 2214.997 kb on + strand, within yehXat 2215.044 kb on - strand, within yehXat 2215.044 kb on - strand, within yehXat 2215.187 kb on - strand, within yehXat 2215.394 kb on + strandat 2215.450 kb on + strandat 2215.461 kb on + strandat 2215.683 kb on - strand, within yehYat 2215.683 kb on - strand, within yehYat 2215.698 kb on - strand, within yehYat 2215.698 kb on - strand, within yehYat 2215.701 kb on - strand, within yehYat 2215.805 kb on + strand, within yehYat 2215.876 kb on - strand, within yehYat 2215.909 kb on - strand, within yehYat 2215.909 kb on - strand, within yehYat 2215.998 kb on + strand, within yehYat 2216.025 kb on + strand, within yehYat 2216.025 kb on + strand, within yehYat 2216.209 kb on + strand, within yehYat 2216.209 kb on + strand, within yehYat 2216.217 kb on - strand, within yehYat 2216.217 kb on - strand, within yehYat 2216.355 kb on - strand, within yehYat 2216.544 kb on - strandat 2216.627 kb on + strandat 2216.745 kb on - strand, within yehZat 2216.745 kb on - strand, within yehZat 2216.748 kb on - strand, within yehZat 2216.767 kb on - strand, within yehZat 2216.782 kb on + strand, within yehZat 2216.950 kb on - strand, within yehZat 2216.950 kb on - strand, within yehZat 2216.955 kb on + strand, within yehZat 2216.955 kb on + strand, within yehZat 2217.019 kb on + strand, within yehZat 2217.153 kb on - strand, within yehZat 2217.153 kb on - strand, within yehZat 2217.260 kb on + strand, within yehZat 2217.311 kb on - strand, within yehZat 2217.311 kb on - strand, within yehZat 2217.328 kb on + strand, within yehZat 2217.376 kb on + strand, within yehZat 2217.463 kb on + strandat 2217.470 kb on + strandat 2217.560 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol%
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2,214,491 + -1.7
2,214,491 + -0.4
2,214,540 + +0.9
2,214,540 + +1.6
2,214,554 - -0.8
2,214,568 - +1.4
2,214,700 + yehX b2129 0.21 -0.1
2,214,708 - yehX b2129 0.22 +0.4
2,214,965 + yehX b2129 0.50 +0.1
2,214,997 + yehX b2129 0.53 -0.3
2,215,044 - yehX b2129 0.58 -0.0
2,215,044 - yehX b2129 0.58 +0.6
2,215,187 - yehX b2129 0.74 -1.2
2,215,394 + -1.5
2,215,450 + -0.9
2,215,461 + +0.9
2,215,683 - yehY b2130 0.23 -1.3
2,215,683 - yehY b2130 0.23 -1.5
2,215,698 - yehY b2130 0.24 +2.5
2,215,698 - yehY b2130 0.24 -2.9
2,215,701 - yehY b2130 0.24 -2.3
2,215,805 + yehY b2130 0.33 -1.1
2,215,876 - yehY b2130 0.39 +0.3
2,215,909 - yehY b2130 0.42 -1.4
2,215,909 - yehY b2130 0.42 -2.2
2,215,998 + yehY b2130 0.50 +0.9
2,216,025 + yehY b2130 0.52 -0.2
2,216,025 + yehY b2130 0.52 +0.1
2,216,209 + yehY b2130 0.68 +0.9
2,216,209 + yehY b2130 0.68 +0.6
2,216,217 - yehY b2130 0.69 -0.1
2,216,217 - yehY b2130 0.69 +1.0
2,216,355 - yehY b2130 0.81 -0.1
2,216,544 - +0.2
2,216,627 + +0.7
2,216,745 - yehZ b2131 0.17 +0.4
2,216,745 - yehZ b2131 0.17 -0.6
2,216,748 - yehZ b2131 0.18 +0.2
2,216,767 - yehZ b2131 0.20 -1.1
2,216,782 + yehZ b2131 0.21 -0.4
2,216,950 - yehZ b2131 0.40 +0.6
2,216,950 - yehZ b2131 0.40 -0.9
2,216,955 + yehZ b2131 0.40 +1.1
2,216,955 + yehZ b2131 0.40 -1.6
2,217,019 + yehZ b2131 0.47 +1.4
2,217,153 - yehZ b2131 0.62 -0.0
2,217,153 - yehZ b2131 0.62 -0.2
2,217,260 + yehZ b2131 0.73 -0.2
2,217,311 - yehZ b2131 0.79 -0.7
2,217,311 - yehZ b2131 0.79 -1.4
2,217,328 + yehZ b2131 0.81 -1.2
2,217,376 + yehZ b2131 0.86 +0.7
2,217,463 + -2.3
2,217,470 + -0.4
2,217,560 - -0.6

Or see this region's nucleotide sequence