Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yehW and yehX are separated by 4 nucleotides yehX and yehY overlap by 8 nucleotides yehY and yehZ are separated by 6 nucleotides
b2128: yehW - predicted transporter subunit: membrane component of ABC superfamily (NCBI), at 2,213,767 to 2,214,498
yehW
b2129: yehX - predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 2,214,503 to 2,215,429
yehX
b2130: yehY - predicted transporter subunit: membrane component of ABC superfamily (NCBI), at 2,215,422 to 2,216,579
yehY
b2131: yehZ - predicted transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 2,216,586 to 2,217,503
yehZ
Position (kb)
2215
2216
2217 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2214.491 kb on + strand at 2214.491 kb on + strand at 2214.540 kb on + strand at 2214.540 kb on + strand at 2214.554 kb on - strand at 2214.568 kb on - strand at 2214.700 kb on + strand, within yehX at 2214.708 kb on - strand, within yehX at 2214.965 kb on + strand, within yehX at 2214.997 kb on + strand, within yehX at 2215.044 kb on - strand, within yehX at 2215.044 kb on - strand, within yehX at 2215.187 kb on - strand, within yehX at 2215.394 kb on + strand at 2215.450 kb on + strand at 2215.461 kb on + strand at 2215.683 kb on - strand, within yehY at 2215.683 kb on - strand, within yehY at 2215.698 kb on - strand, within yehY at 2215.698 kb on - strand, within yehY at 2215.701 kb on - strand, within yehY at 2215.805 kb on + strand, within yehY at 2215.876 kb on - strand, within yehY at 2215.909 kb on - strand, within yehY at 2215.909 kb on - strand, within yehY at 2215.998 kb on + strand, within yehY at 2216.025 kb on + strand, within yehY at 2216.025 kb on + strand, within yehY at 2216.209 kb on + strand, within yehY at 2216.209 kb on + strand, within yehY at 2216.217 kb on - strand, within yehY at 2216.217 kb on - strand, within yehY at 2216.355 kb on - strand, within yehY at 2216.544 kb on - strand at 2216.627 kb on + strand at 2216.745 kb on - strand, within yehZ at 2216.745 kb on - strand, within yehZ at 2216.748 kb on - strand, within yehZ at 2216.767 kb on - strand, within yehZ at 2216.782 kb on + strand, within yehZ at 2216.950 kb on - strand, within yehZ at 2216.950 kb on - strand, within yehZ at 2216.955 kb on + strand, within yehZ at 2216.955 kb on + strand, within yehZ at 2217.019 kb on + strand, within yehZ at 2217.153 kb on - strand, within yehZ at 2217.153 kb on - strand, within yehZ at 2217.260 kb on + strand, within yehZ at 2217.311 kb on - strand, within yehZ at 2217.311 kb on - strand, within yehZ at 2217.328 kb on + strand, within yehZ at 2217.376 kb on + strand, within yehZ at 2217.463 kb on + strand at 2217.470 kb on + strand at 2217.560 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 2,214,491 + -1.7 2,214,491 + -0.4 2,214,540 + +0.9 2,214,540 + +1.6 2,214,554 - -0.8 2,214,568 - +1.4 2,214,700 + yehX b2129 0.21 -0.1 2,214,708 - yehX b2129 0.22 +0.4 2,214,965 + yehX b2129 0.50 +0.1 2,214,997 + yehX b2129 0.53 -0.3 2,215,044 - yehX b2129 0.58 -0.0 2,215,044 - yehX b2129 0.58 +0.6 2,215,187 - yehX b2129 0.74 -1.2 2,215,394 + -1.5 2,215,450 + -0.9 2,215,461 + +0.9 2,215,683 - yehY b2130 0.23 -1.3 2,215,683 - yehY b2130 0.23 -1.5 2,215,698 - yehY b2130 0.24 +2.5 2,215,698 - yehY b2130 0.24 -2.9 2,215,701 - yehY b2130 0.24 -2.3 2,215,805 + yehY b2130 0.33 -1.1 2,215,876 - yehY b2130 0.39 +0.3 2,215,909 - yehY b2130 0.42 -1.4 2,215,909 - yehY b2130 0.42 -2.2 2,215,998 + yehY b2130 0.50 +0.9 2,216,025 + yehY b2130 0.52 -0.2 2,216,025 + yehY b2130 0.52 +0.1 2,216,209 + yehY b2130 0.68 +0.9 2,216,209 + yehY b2130 0.68 +0.6 2,216,217 - yehY b2130 0.69 -0.1 2,216,217 - yehY b2130 0.69 +1.0 2,216,355 - yehY b2130 0.81 -0.1 2,216,544 - +0.2 2,216,627 + +0.7 2,216,745 - yehZ b2131 0.17 +0.4 2,216,745 - yehZ b2131 0.17 -0.6 2,216,748 - yehZ b2131 0.18 +0.2 2,216,767 - yehZ b2131 0.20 -1.1 2,216,782 + yehZ b2131 0.21 -0.4 2,216,950 - yehZ b2131 0.40 +0.6 2,216,950 - yehZ b2131 0.40 -0.9 2,216,955 + yehZ b2131 0.40 +1.1 2,216,955 + yehZ b2131 0.40 -1.6 2,217,019 + yehZ b2131 0.47 +1.4 2,217,153 - yehZ b2131 0.62 -0.0 2,217,153 - yehZ b2131 0.62 -0.2 2,217,260 + yehZ b2131 0.73 -0.2 2,217,311 - yehZ b2131 0.79 -0.7 2,217,311 - yehZ b2131 0.79 -1.4 2,217,328 + yehZ b2131 0.81 -1.2 2,217,376 + yehZ b2131 0.86 +0.7 2,217,463 + -2.3 2,217,470 + -0.4 2,217,560 - -0.6
Or see this region's nucleotide sequence