Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaI and wcaH overlap by 4 nucleotides wcaH and fcl are separated by 2 nucleotides fcl and gmd are separated by 2 nucleotides
b2050: wcaI - predicted glycosyl transferase (NCBI), at 2,122,547 to 2,123,770
wcaI
b2051: wcaH - GDP-mannose mannosyl hydrolase (VIMSS), at 2,123,767 to 2,124,246
wcaH
b2052: fcl - bifunctional GDP-fucose synthetase: GDP-4-dehydro-6-deoxy-D-mannose epimerase/ GDP-4-dehydro-6-L-deoxygalactose reductase (NCBI), at 2,124,249 to 2,125,214
fcl
b2053: gmd - GDP-D-mannose dehydratase, NAD(P)-binding (NCBI), at 2,125,217 to 2,126,338
gmd
Position (kb)
2124
2125
2126 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2123.290 kb on + strand, within wcaI at 2123.290 kb on + strand, within wcaI at 2123.479 kb on + strand, within wcaI at 2123.610 kb on + strand, within wcaI at 2123.668 kb on + strand at 2123.822 kb on + strand, within wcaH at 2124.177 kb on - strand, within wcaH at 2124.282 kb on + strand at 2124.282 kb on + strand at 2124.465 kb on + strand, within fcl at 2124.601 kb on + strand, within fcl at 2124.630 kb on + strand, within fcl at 2124.630 kb on + strand, within fcl at 2124.722 kb on - strand, within fcl at 2124.813 kb on - strand, within fcl at 2124.848 kb on - strand, within fcl at 2124.896 kb on + strand, within fcl at 2124.951 kb on + strand, within fcl at 2124.951 kb on + strand, within fcl at 2125.046 kb on - strand, within fcl at 2125.113 kb on + strand, within fcl at 2125.189 kb on + strand at 2125.221 kb on - strand at 2125.556 kb on + strand, within gmd at 2125.566 kb on + strand, within gmd at 2125.570 kb on + strand, within gmd at 2125.570 kb on + strand, within gmd at 2125.643 kb on + strand, within gmd at 2125.706 kb on + strand, within gmd at 2125.707 kb on - strand, within gmd at 2125.797 kb on + strand, within gmd at 2125.797 kb on + strand, within gmd at 2125.828 kb on + strand, within gmd at 2125.887 kb on + strand, within gmd at 2125.941 kb on - strand, within gmd at 2126.048 kb on + strand, within gmd at 2126.090 kb on - strand, within gmd at 2126.093 kb on - strand, within gmd at 2126.151 kb on + strand, within gmd at 2126.181 kb on - strand, within gmd
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 2,123,290 + wcaI b2050 0.61 -1.5 2,123,290 + wcaI b2050 0.61 +0.9 2,123,479 + wcaI b2050 0.76 -0.1 2,123,610 + wcaI b2050 0.87 +0.5 2,123,668 + -1.8 2,123,822 + wcaH b2051 0.11 -1.7 2,124,177 - wcaH b2051 0.85 -1.3 2,124,282 + +0.2 2,124,282 + +0.7 2,124,465 + fcl b2052 0.22 +0.0 2,124,601 + fcl b2052 0.36 -0.1 2,124,630 + fcl b2052 0.39 -1.3 2,124,630 + fcl b2052 0.39 +0.3 2,124,722 - fcl b2052 0.49 -1.1 2,124,813 - fcl b2052 0.58 -1.8 2,124,848 - fcl b2052 0.62 -0.5 2,124,896 + fcl b2052 0.67 -1.0 2,124,951 + fcl b2052 0.73 -0.5 2,124,951 + fcl b2052 0.73 -1.4 2,125,046 - fcl b2052 0.83 -2.0 2,125,113 + fcl b2052 0.89 +0.5 2,125,189 + +0.7 2,125,221 - -1.7 2,125,556 + gmd b2053 0.30 +0.1 2,125,566 + gmd b2053 0.31 -1.0 2,125,570 + gmd b2053 0.31 +0.4 2,125,570 + gmd b2053 0.31 -0.7 2,125,643 + gmd b2053 0.38 -1.6 2,125,706 + gmd b2053 0.44 +0.5 2,125,707 - gmd b2053 0.44 -0.0 2,125,797 + gmd b2053 0.52 -0.1 2,125,797 + gmd b2053 0.52 +0.6 2,125,828 + gmd b2053 0.54 -3.2 2,125,887 + gmd b2053 0.60 +0.8 2,125,941 - gmd b2053 0.65 -0.2 2,126,048 + gmd b2053 0.74 -1.1 2,126,090 - gmd b2053 0.78 -1.0 2,126,093 - gmd b2053 0.78 +0.9 2,126,151 + gmd b2053 0.83 +0.3 2,126,181 - gmd b2053 0.86 +0.1
Or see this region's nucleotide sequence