Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliK and fliL are separated by 104 nucleotides fliL and fliM are separated by 4 nucleotides fliM and fliN overlap by 4 nucleotides fliN and fliO are separated by 2 nucleotides fliO and fliP overlap by 1 nucleotides
b1943: fliK - flagellar hook-length control protein (NCBI), at 2,016,410 to 2,017,537
fliK
b1944: fliL - flagellar basal body-associated protein FliL (NCBI), at 2,017,642 to 2,018,106
fliL
b1945: fliM - flagellar motor switch protein M (NCBI), at 2,018,111 to 2,019,115
fliM
b1946: fliN - flagellar motor switch protein (NCBI), at 2,019,112 to 2,019,525
fliN
b1947: fliO - flagellar biosynthesis (VIMSS), at 2,019,528 to 2,019,893
fliO
b1948: fliP - flagellar biosynthesis protein P (NCBI), at 2,019,893 to 2,020,630
fliP
Position (kb)
2018
2019
2020 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2017.116 kb on + strand, within fliK at 2017.182 kb on + strand, within fliK at 2017.207 kb on + strand, within fliK at 2017.207 kb on + strand, within fliK at 2017.267 kb on + strand, within fliK at 2017.323 kb on + strand, within fliK at 2017.488 kb on + strand at 2017.757 kb on + strand, within fliL at 2017.757 kb on + strand, within fliL at 2017.797 kb on - strand, within fliL at 2017.813 kb on + strand, within fliL at 2017.947 kb on - strand, within fliL at 2017.972 kb on + strand, within fliL at 2018.029 kb on - strand, within fliL at 2018.038 kb on - strand, within fliL at 2018.038 kb on - strand, within fliL at 2018.299 kb on + strand, within fliM at 2018.411 kb on + strand, within fliM at 2018.420 kb on - strand, within fliM at 2018.466 kb on + strand, within fliM at 2018.466 kb on + strand, within fliM at 2018.608 kb on - strand, within fliM at 2018.608 kb on - strand, within fliM at 2018.610 kb on - strand, within fliM at 2018.610 kb on - strand, within fliM at 2018.652 kb on - strand, within fliM at 2018.689 kb on - strand, within fliM at 2018.689 kb on - strand, within fliM at 2018.700 kb on + strand, within fliM at 2018.870 kb on + strand, within fliM at 2018.875 kb on + strand, within fliM at 2018.890 kb on - strand, within fliM at 2019.025 kb on + strand at 2019.106 kb on + strand at 2019.397 kb on + strand, within fliN at 2019.437 kb on + strand, within fliN at 2019.538 kb on + strand at 2019.538 kb on + strand at 2019.995 kb on + strand, within fliP at 2020.000 kb on + strand, within fliP
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 2,017,116 + fliK b1943 0.63 +1.4 2,017,182 + fliK b1943 0.68 -1.0 2,017,207 + fliK b1943 0.71 -1.3 2,017,207 + fliK b1943 0.71 +2.0 2,017,267 + fliK b1943 0.76 +0.5 2,017,323 + fliK b1943 0.81 +1.4 2,017,488 + -0.4 2,017,757 + fliL b1944 0.25 -0.7 2,017,757 + fliL b1944 0.25 +1.8 2,017,797 - fliL b1944 0.33 +0.4 2,017,813 + fliL b1944 0.37 +0.5 2,017,947 - fliL b1944 0.66 +0.8 2,017,972 + fliL b1944 0.71 -0.2 2,018,029 - fliL b1944 0.83 +0.4 2,018,038 - fliL b1944 0.85 -0.5 2,018,038 - fliL b1944 0.85 -0.6 2,018,299 + fliM b1945 0.19 +0.8 2,018,411 + fliM b1945 0.30 -1.1 2,018,420 - fliM b1945 0.31 +0.2 2,018,466 + fliM b1945 0.35 -0.1 2,018,466 + fliM b1945 0.35 +0.1 2,018,608 - fliM b1945 0.49 -1.3 2,018,608 - fliM b1945 0.49 -2.0 2,018,610 - fliM b1945 0.50 +0.9 2,018,610 - fliM b1945 0.50 -0.8 2,018,652 - fliM b1945 0.54 -2.1 2,018,689 - fliM b1945 0.58 -0.2 2,018,689 - fliM b1945 0.58 -2.3 2,018,700 + fliM b1945 0.59 +0.7 2,018,870 + fliM b1945 0.76 -1.3 2,018,875 + fliM b1945 0.76 +0.1 2,018,890 - fliM b1945 0.78 -0.8 2,019,025 + +0.5 2,019,106 + +0.7 2,019,397 + fliN b1946 0.69 +0.3 2,019,437 + fliN b1946 0.79 -0.7 2,019,538 + +0.7 2,019,538 + -1.6 2,019,995 + fliP b1948 0.14 +0.2 2,020,000 + fliP b1948 0.14 -0.7
Or see this region's nucleotide sequence