Strain Fitness in Escherichia coli BW25113 around b1807

Experiment: 3-methyl-1-butanol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfadD and yeaY are separated by 204 nucleotidesyeaY and yeaZ are separated by 39 nucleotidesyeaZ and yoaA are separated by 57 nucleotides b1805: fadD - acyl-CoA synthase (NCBI), at 1,886,085 to 1,887,770 fadD b1806: yeaY - predicted lipoprotein (NCBI), at 1,887,975 to 1,888,556 yeaY b1807: yeaZ - predicted peptidase (NCBI), at 1,888,596 to 1,889,291 yeaZ b1808: yoaA - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 1,889,349 to 1,891,259 yoaA Position (kb) 1888 1889 1890Strain fitness (log2 ratio) -1 0 1 2at 1887.762 kb on - strandat 1887.981 kb on + strandat 1888.207 kb on + strand, within yeaYat 1888.428 kb on - strand, within yeaYat 1888.428 kb on - strand, within yeaYat 1888.463 kb on + strand, within yeaYat 1888.586 kb on + strandat 1888.586 kb on + strandat 1889.333 kb on - strandat 1889.364 kb on - strandat 1889.751 kb on + strand, within yoaAat 1889.811 kb on - strand, within yoaAat 1889.917 kb on + strand, within yoaAat 1889.917 kb on + strand, within yoaAat 1890.135 kb on - strand, within yoaAat 1890.148 kb on - strand, within yoaAat 1890.187 kb on + strand, within yoaA

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol%
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1,887,762 - +0.2
1,887,981 + -0.2
1,888,207 + yeaY b1806 0.40 +0.2
1,888,428 - yeaY b1806 0.78 -0.5
1,888,428 - yeaY b1806 0.78 -0.4
1,888,463 + yeaY b1806 0.84 -0.6
1,888,586 + -0.7
1,888,586 + -0.2
1,889,333 - +1.8
1,889,364 - -0.2
1,889,751 + yoaA b1808 0.21 -0.6
1,889,811 - yoaA b1808 0.24 +1.3
1,889,917 + yoaA b1808 0.30 +1.4
1,889,917 + yoaA b1808 0.30 -0.6
1,890,135 - yoaA b1808 0.41 -0.2
1,890,148 - yoaA b1808 0.42 -1.0
1,890,187 + yoaA b1808 0.44 +1.2

Or see this region's nucleotide sequence