Strain Fitness in Escherichia coli BW25113 around b1621

Experiment: 3-methyl-1-butanol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmalI and malX are separated by 174 nucleotidesmalX and malY are separated by 9 nucleotides b1620: malI - DNA-binding transcriptional repressor (NCBI), at 1,696,176 to 1,697,204 malI b1621: malX - fused maltose and glucose-specific PTS enzymes: IIB component -! IIC component (NCBI), at 1,697,379 to 1,698,971 malX b1622: malY - bifunctional beta-cystathionase, PLP-dependent/ regulator of maltose regulon (NCBI), at 1,698,981 to 1,700,153 malY Position (kb) 1697 1698 1699Strain fitness (log2 ratio) -2 -1 0 1 2at 1696.599 kb on - strand, within malIat 1696.617 kb on + strand, within malIat 1696.643 kb on + strand, within malIat 1697.394 kb on + strandat 1697.437 kb on - strandat 1697.538 kb on - strandat 1697.538 kb on - strandat 1697.580 kb on - strand, within malXat 1697.580 kb on - strand, within malXat 1697.869 kb on - strand, within malXat 1698.219 kb on - strand, within malXat 1698.303 kb on + strand, within malXat 1698.450 kb on + strand, within malXat 1698.450 kb on + strand, within malXat 1698.495 kb on + strand, within malXat 1698.716 kb on - strand, within malXat 1698.730 kb on - strand, within malXat 1698.918 kb on - strandat 1698.950 kb on + strandat 1698.950 kb on + strandat 1698.952 kb on + strandat 1699.029 kb on - strandat 1699.060 kb on + strandat 1699.068 kb on - strandat 1699.068 kb on - strandat 1699.152 kb on + strand, within malYat 1699.279 kb on + strand, within malYat 1699.279 kb on + strand, within malYat 1699.546 kb on + strand, within malYat 1699.546 kb on + strand, within malYat 1699.566 kb on - strand, within malYat 1699.796 kb on + strand, within malY

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol%
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1,696,599 - malI b1620 0.41 +1.1
1,696,617 + malI b1620 0.43 +0.2
1,696,643 + malI b1620 0.45 -1.8
1,697,394 + +0.4
1,697,437 - -0.1
1,697,538 - +1.0
1,697,538 - +1.5
1,697,580 - malX b1621 0.13 +0.3
1,697,580 - malX b1621 0.13 +2.4
1,697,869 - malX b1621 0.31 -0.1
1,698,219 - malX b1621 0.53 -0.3
1,698,303 + malX b1621 0.58 -1.8
1,698,450 + malX b1621 0.67 +0.1
1,698,450 + malX b1621 0.67 -1.1
1,698,495 + malX b1621 0.70 +0.7
1,698,716 - malX b1621 0.84 +2.2
1,698,730 - malX b1621 0.85 +0.7
1,698,918 - -0.2
1,698,950 + -0.1
1,698,950 + -1.1
1,698,952 + +1.7
1,699,029 - +0.0
1,699,060 + -2.2
1,699,068 - -0.9
1,699,068 - +0.4
1,699,152 + malY b1622 0.15 -0.5
1,699,279 + malY b1622 0.25 +0.2
1,699,279 + malY b1622 0.25 +1.0
1,699,546 + malY b1622 0.48 -0.2
1,699,546 + malY b1622 0.48 -0.5
1,699,566 - malY b1622 0.50 -2.2
1,699,796 + malY b1622 0.69 -1.4

Or see this region's nucleotide sequence