Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ddpB and ddpA are separated by 1 nucleotides ddpA and ddpX are separated by 13 nucleotides ddpX and b1489 are separated by 257 nucleotides
b1486: ddpB - D-ala-D-ala transporter subunit (NCBI), at 1,557,931 to 1,558,953
ddpB
b1487: ddpA - D-ala-D-a la transporter subunit (NCBI), at 1,558,955 to 1,560,505
ddpA
b1488: ddpX - D-ala-D-ala dipeptidase, Zn-dependent (NCBI), at 1,560,519 to 1,561,100
ddpX
b1489: b1489 - putative enzyme (VIMSS), at 1,561,358 to 1,563,757
b1489
Position (kb)
1558
1559
1560
1561 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1558.000 kb on - strand at 1558.010 kb on - strand at 1558.182 kb on - strand, within ddpB at 1558.182 kb on - strand, within ddpB at 1558.226 kb on + strand, within ddpB at 1558.573 kb on - strand, within ddpB at 1558.909 kb on + strand at 1558.909 kb on + strand at 1558.940 kb on - strand at 1559.057 kb on - strand at 1559.057 kb on - strand at 1559.426 kb on - strand, within ddpA at 1559.525 kb on - strand, within ddpA at 1559.596 kb on - strand, within ddpA at 1559.703 kb on - strand, within ddpA at 1559.785 kb on - strand, within ddpA at 1559.785 kb on - strand, within ddpA at 1560.049 kb on - strand, within ddpA at 1560.382 kb on - strand at 1560.658 kb on - strand, within ddpX at 1560.709 kb on + strand, within ddpX at 1560.869 kb on - strand, within ddpX at 1560.911 kb on + strand, within ddpX at 1561.147 kb on - strand at 1561.147 kb on - strand at 1561.159 kb on - strand at 1561.463 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 1,558,000 - -0.2 1,558,010 - -0.8 1,558,182 - ddpB b1486 0.25 -1.8 1,558,182 - ddpB b1486 0.25 -0.3 1,558,226 + ddpB b1486 0.29 +1.1 1,558,573 - ddpB b1486 0.63 -1.0 1,558,909 + +0.2 1,558,909 + +2.3 1,558,940 - -1.0 1,559,057 - +0.3 1,559,057 - -0.0 1,559,426 - ddpA b1487 0.30 -0.0 1,559,525 - ddpA b1487 0.37 +1.6 1,559,596 - ddpA b1487 0.41 +1.7 1,559,703 - ddpA b1487 0.48 +0.6 1,559,785 - ddpA b1487 0.54 +0.7 1,559,785 - ddpA b1487 0.54 +0.0 1,560,049 - ddpA b1487 0.71 +1.7 1,560,382 - +1.0 1,560,658 - ddpX b1488 0.24 -1.2 1,560,709 + ddpX b1488 0.33 +0.1 1,560,869 - ddpX b1488 0.60 +0.9 1,560,911 + ddpX b1488 0.67 +0.3 1,561,147 - +1.2 1,561,147 - +1.7 1,561,159 - -1.3 1,561,463 - +2.3
Or see this region's nucleotide sequence