Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycjW and ycjX are separated by 155 nucleotides ycjX and ycjF overlap by 4 nucleotides
b1320: ycjW - predicted DNA-binding transcriptional regulator (NCBI), at 1,380,987 to 1,381,985
ycjW
b1321: ycjX - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 1,382,141 to 1,383,538
ycjX
b1322: ycjF - hypothetical protein (NCBI), at 1,383,535 to 1,384,596
ycjF
Position (kb)
1382
1383
1384 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1381.213 kb on - strand, within ycjW at 1381.228 kb on - strand, within ycjW at 1381.340 kb on - strand, within ycjW at 1381.383 kb on - strand, within ycjW at 1381.406 kb on - strand, within ycjW at 1381.406 kb on - strand, within ycjW at 1381.452 kb on - strand, within ycjW at 1381.464 kb on - strand, within ycjW at 1381.522 kb on - strand, within ycjW at 1381.529 kb on - strand, within ycjW at 1381.529 kb on - strand, within ycjW at 1381.590 kb on + strand, within ycjW at 1381.600 kb on - strand, within ycjW at 1381.610 kb on - strand, within ycjW at 1381.610 kb on - strand, within ycjW at 1381.612 kb on - strand, within ycjW at 1381.612 kb on - strand, within ycjW at 1381.693 kb on - strand, within ycjW at 1381.693 kb on - strand, within ycjW at 1381.727 kb on - strand, within ycjW at 1381.731 kb on - strand, within ycjW at 1381.747 kb on - strand at 1381.790 kb on - strand, within ycjW at 1381.790 kb on - strand, within ycjW at 1381.790 kb on - strand, within ycjW at 1381.793 kb on + strand, within ycjW at 1381.987 kb on + strand at 1382.128 kb on + strand at 1382.203 kb on - strand at 1382.203 kb on - strand at 1382.625 kb on - strand, within ycjX at 1382.756 kb on - strand, within ycjX at 1382.767 kb on - strand, within ycjX at 1382.860 kb on - strand, within ycjX at 1382.860 kb on - strand, within ycjX at 1383.086 kb on + strand, within ycjX at 1383.211 kb on - strand, within ycjX at 1383.269 kb on - strand, within ycjX at 1383.365 kb on - strand, within ycjX at 1383.365 kb on - strand, within ycjX at 1383.367 kb on - strand, within ycjX at 1383.572 kb on + strand at 1383.781 kb on + strand, within ycjF at 1383.801 kb on - strand, within ycjF at 1383.801 kb on - strand, within ycjF at 1383.828 kb on - strand, within ycjF at 1383.892 kb on + strand, within ycjF at 1383.892 kb on + strand, within ycjF at 1383.937 kb on - strand, within ycjF at 1384.032 kb on + strand, within ycjF at 1384.032 kb on + strand, within ycjF at 1384.214 kb on + strand, within ycjF at 1384.214 kb on + strand, within ycjF at 1384.420 kb on + strand, within ycjF at 1384.428 kb on - strand, within ycjF at 1384.428 kb on - strand, within ycjF at 1384.428 kb on - strand, within ycjF
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 1,381,213 - ycjW b1320 0.23 -1.1 1,381,228 - ycjW b1320 0.24 +2.5 1,381,340 - ycjW b1320 0.35 -0.7 1,381,383 - ycjW b1320 0.40 +1.7 1,381,406 - ycjW b1320 0.42 -1.6 1,381,406 - ycjW b1320 0.42 +1.1 1,381,452 - ycjW b1320 0.47 +1.1 1,381,464 - ycjW b1320 0.48 -3.4 1,381,522 - ycjW b1320 0.54 -0.1 1,381,529 - ycjW b1320 0.54 +0.5 1,381,529 - ycjW b1320 0.54 +0.9 1,381,590 + ycjW b1320 0.60 -2.4 1,381,600 - ycjW b1320 0.61 +0.2 1,381,610 - ycjW b1320 0.62 +0.4 1,381,610 - ycjW b1320 0.62 -0.7 1,381,612 - ycjW b1320 0.63 +0.6 1,381,612 - ycjW b1320 0.63 +0.2 1,381,693 - ycjW b1320 0.71 -0.2 1,381,693 - ycjW b1320 0.71 +1.8 1,381,727 - ycjW b1320 0.74 +0.9 1,381,731 - ycjW b1320 0.74 +0.6 1,381,747 - +0.8 1,381,790 - ycjW b1320 0.80 +0.3 1,381,790 - ycjW b1320 0.80 +2.0 1,381,790 - ycjW b1320 0.80 +0.2 1,381,793 + ycjW b1320 0.81 +1.2 1,381,987 + -2.4 1,382,128 + -0.5 1,382,203 - +2.5 1,382,203 - -1.3 1,382,625 - ycjX b1321 0.35 +1.1 1,382,756 - ycjX b1321 0.44 +0.2 1,382,767 - ycjX b1321 0.45 +0.6 1,382,860 - ycjX b1321 0.51 +0.8 1,382,860 - ycjX b1321 0.51 +0.4 1,383,086 + ycjX b1321 0.68 +0.8 1,383,211 - ycjX b1321 0.77 -0.3 1,383,269 - ycjX b1321 0.81 +0.6 1,383,365 - ycjX b1321 0.88 +3.2 1,383,365 - ycjX b1321 0.88 -1.0 1,383,367 - ycjX b1321 0.88 +1.7 1,383,572 + +1.8 1,383,781 + ycjF b1322 0.23 +0.1 1,383,801 - ycjF b1322 0.25 +0.5 1,383,801 - ycjF b1322 0.25 -0.4 1,383,828 - ycjF b1322 0.28 -1.4 1,383,892 + ycjF b1322 0.34 -0.8 1,383,892 + ycjF b1322 0.34 -2.1 1,383,937 - ycjF b1322 0.38 +1.6 1,384,032 + ycjF b1322 0.47 -1.2 1,384,032 + ycjF b1322 0.47 +0.1 1,384,214 + ycjF b1322 0.64 +0.4 1,384,214 + ycjF b1322 0.64 +0.1 1,384,420 + ycjF b1322 0.83 -0.9 1,384,428 - ycjF b1322 0.84 +1.3 1,384,428 - ycjF b1322 0.84 +0.2 1,384,428 - ycjF b1322 0.84 +0.5
Or see this region's nucleotide sequence