Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycjQ and b1314 are separated by 18 nucleotides b1314 and ycjS are separated by 9 nucleotides ycjS and ycjT overlap by 4 nucleotides
b1313: ycjQ - predicted oxidoreductase, Zn-dependent and NAD(P)-binding (NCBI), at 1,372,987 to 1,374,039
ycjQ
b1314: b1314 - putative transient receptor potential locus (VIMSS), at 1,374,058 to 1,374,846
b1314
b1315: ycjS - predicted oxidoreductase, NADH-binding (NCBI), at 1,374,856 to 1,375,911
ycjS
b1316: ycjT - predicted hydrolase (NCBI), at 1,375,908 to 1,378,175
ycjT
Position (kb)
1374
1375
1376 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1373.883 kb on + strand, within ycjQ at 1374.246 kb on - strand, within b1314 at 1374.292 kb on + strand, within b1314 at 1374.325 kb on + strand, within b1314 at 1374.416 kb on - strand, within b1314 at 1374.662 kb on + strand, within b1314 at 1374.708 kb on - strand, within b1314 at 1374.759 kb on + strand, within b1314 at 1374.769 kb on - strand at 1374.769 kb on - strand at 1374.907 kb on + strand at 1375.200 kb on + strand, within ycjS at 1375.420 kb on + strand, within ycjS at 1375.426 kb on - strand, within ycjS at 1375.566 kb on + strand, within ycjS at 1375.566 kb on + strand, within ycjS at 1375.566 kb on + strand, within ycjS at 1375.701 kb on + strand, within ycjS at 1375.709 kb on - strand, within ycjS at 1375.709 kb on - strand, within ycjS at 1375.842 kb on - strand at 1375.872 kb on + strand at 1375.872 kb on + strand at 1375.985 kb on + strand at 1375.985 kb on + strand at 1376.172 kb on + strand, within ycjT at 1376.392 kb on - strand, within ycjT at 1376.562 kb on - strand, within ycjT at 1376.589 kb on - strand, within ycjT at 1376.589 kb on - strand, within ycjT
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 1,373,883 + ycjQ b1313 0.85 -1.0 1,374,246 - b1314 b1314 0.24 +0.3 1,374,292 + b1314 b1314 0.30 -0.8 1,374,325 + b1314 b1314 0.34 -0.4 1,374,416 - b1314 b1314 0.45 +0.6 1,374,662 + b1314 b1314 0.77 +2.0 1,374,708 - b1314 b1314 0.82 +1.0 1,374,759 + b1314 b1314 0.89 +0.1 1,374,769 - -0.0 1,374,769 - +0.8 1,374,907 + -2.0 1,375,200 + ycjS b1315 0.33 +1.3 1,375,420 + ycjS b1315 0.53 -0.8 1,375,426 - ycjS b1315 0.54 -1.6 1,375,566 + ycjS b1315 0.67 -1.1 1,375,566 + ycjS b1315 0.67 +0.3 1,375,566 + ycjS b1315 0.67 -1.1 1,375,701 + ycjS b1315 0.80 -0.6 1,375,709 - ycjS b1315 0.81 -1.5 1,375,709 - ycjS b1315 0.81 -0.3 1,375,842 - +0.9 1,375,872 + -0.5 1,375,872 + -1.4 1,375,985 + +0.5 1,375,985 + +0.5 1,376,172 + ycjT b1316 0.12 -1.0 1,376,392 - ycjT b1316 0.21 -0.4 1,376,562 - ycjT b1316 0.29 +0.9 1,376,589 - ycjT b1316 0.30 +0.8 1,376,589 - ycjT b1316 0.30 -1.4
Or see this region's nucleotide sequence