Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycbB and ycbK are separated by 180 nucleotides ycbK and ycbL are separated by 26 nucleotides ycbL and aspC are separated by 221 nucleotides
b0925: ycbB - predicted carboxypeptidase (NCBI), at 980,270 to 982,117
ycbB
b0926: ycbK - hypothetical protein (NCBI), at 982,298 to 982,846
ycbK
b0927: ycbL - predicted metal-binding enzyme (NCBI), at 982,873 to 983,520
ycbL
b0928: aspC - aspartate aminotransferase, PLP-dependent (NCBI), at 983,742 to 984,932
aspC
Position (kb) 982
983
984 Strain fitness (log2 ratio) -2
-1
0
1
2 at 981.888 kb on - strand, within ycbB at 981.888 kb on - strand, within ycbB at 981.898 kb on - strand, within ycbB at 981.989 kb on + strand at 981.989 kb on + strand at 982.014 kb on - strand at 982.033 kb on + strand at 982.046 kb on - strand at 982.046 kb on - strand at 982.241 kb on + strand at 982.400 kb on - strand, within ycbK at 982.400 kb on - strand, within ycbK at 982.679 kb on + strand, within ycbK at 982.719 kb on + strand, within ycbK at 982.765 kb on - strand, within ycbK at 982.779 kb on + strand, within ycbK at 982.790 kb on - strand, within ycbK at 982.792 kb on - strand at 982.818 kb on - strand at 982.850 kb on - strand at 983.009 kb on + strand, within ycbL at 983.020 kb on + strand, within ycbL at 983.020 kb on + strand, within ycbL at 983.050 kb on - strand, within ycbL at 983.050 kb on - strand, within ycbL at 983.052 kb on - strand, within ycbL at 983.052 kb on - strand, within ycbL at 983.423 kb on - strand, within ycbL at 983.522 kb on - strand at 983.586 kb on - strand at 983.691 kb on - strand at 983.786 kb on - strand at 983.786 kb on - strand at 983.884 kb on + strand, within aspC at 983.983 kb on - strand, within aspC at 984.232 kb on - strand, within aspC at 984.326 kb on + strand, within aspC at 984.365 kb on - strand, within aspC at 984.402 kb on + strand, within aspC at 984.402 kb on + strand, within aspC
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 981,888 - ycbB b0925 0.88 +0.6 981,888 - ycbB b0925 0.88 +0.8 981,898 - ycbB b0925 0.88 +1.9 981,989 + -1.0 981,989 + -1.4 982,014 - +0.5 982,033 + -0.8 982,046 - -2.2 982,046 - -1.0 982,241 + -2.2 982,400 - ycbK b0926 0.19 +1.7 982,400 - ycbK b0926 0.19 -0.1 982,679 + ycbK b0926 0.69 -1.2 982,719 + ycbK b0926 0.77 -0.6 982,765 - ycbK b0926 0.85 -0.1 982,779 + ycbK b0926 0.88 +0.1 982,790 - ycbK b0926 0.90 -1.6 982,792 - -0.1 982,818 - +0.8 982,850 - +1.5 983,009 + ycbL b0927 0.21 +0.2 983,020 + ycbL b0927 0.23 -2.1 983,020 + ycbL b0927 0.23 +0.4 983,050 - ycbL b0927 0.27 +2.4 983,050 - ycbL b0927 0.27 -1.0 983,052 - ycbL b0927 0.28 -2.6 983,052 - ycbL b0927 0.28 -1.5 983,423 - ycbL b0927 0.85 -0.0 983,522 - -0.5 983,586 - +1.2 983,691 - +0.3 983,786 - -0.5 983,786 - -1.2 983,884 + aspC b0928 0.12 +0.7 983,983 - aspC b0928 0.20 +0.2 984,232 - aspC b0928 0.41 +1.7 984,326 + aspC b0928 0.49 +1.2 984,365 - aspC b0928 0.52 +0.6 984,402 + aspC b0928 0.55 -0.3 984,402 + aspC b0928 0.55 -0.5
Or see this region's nucleotide sequence