Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybhM and ybhN are separated by 35 nucleotides ybhN and ybhO overlap by 1 nucleotides ybhO and ybhP overlap by 4 nucleotides ybhP and ybhQ are separated by 132 nucleotides
b0787: ybhM - conserved inner membrane protein (NCBI), at 820,016 to 820,729
ybhM
b0788: ybhN - conserved inner membrane protein (NCBI), at 820,765 to 821,721
ybhN
b0789: ybhO - cardiolipin synthase 2 (NCBI), at 821,721 to 822,962
ybhO
b0790: ybhP - predicted DNase (NCBI), at 822,959 to 823,720
ybhP
b0791: ybhQ - predicted inner membrane protein (NCBI), at 823,853 to 824,263
ybhQ
Position (kb) 821
822
823 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 820.826 kb on - strand at 820.826 kb on - strand at 820.826 kb on - strand at 820.926 kb on - strand, within ybhN at 821.003 kb on + strand, within ybhN at 821.004 kb on - strand, within ybhN at 821.158 kb on - strand, within ybhN at 821.314 kb on - strand, within ybhN at 821.329 kb on + strand, within ybhN at 821.333 kb on - strand, within ybhN at 821.435 kb on - strand, within ybhN at 821.615 kb on - strand, within ybhN at 821.666 kb on - strand at 821.850 kb on - strand, within ybhO at 821.898 kb on - strand, within ybhO at 821.907 kb on - strand, within ybhO at 822.100 kb on + strand, within ybhO at 822.100 kb on + strand, within ybhO at 822.155 kb on - strand, within ybhO at 822.188 kb on - strand, within ybhO at 822.188 kb on - strand, within ybhO at 822.273 kb on + strand, within ybhO at 822.328 kb on - strand, within ybhO at 822.328 kb on - strand, within ybhO at 822.553 kb on + strand, within ybhO at 822.553 kb on + strand, within ybhO at 822.780 kb on + strand, within ybhO at 822.780 kb on + strand, within ybhO at 822.849 kb on + strand at 822.891 kb on - strand at 823.224 kb on + strand, within ybhP at 823.224 kb on + strand, within ybhP at 823.296 kb on - strand, within ybhP at 823.296 kb on - strand, within ybhP at 823.302 kb on - strand, within ybhP at 823.331 kb on + strand, within ybhP at 823.346 kb on + strand, within ybhP at 823.405 kb on + strand, within ybhP at 823.434 kb on - strand, within ybhP at 823.454 kb on - strand, within ybhP at 823.604 kb on - strand, within ybhP at 823.653 kb on - strand at 823.850 kb on + strand at 823.893 kb on - strand at 823.947 kb on + strand, within ybhQ at 823.961 kb on - strand, within ybhQ
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 820,826 - +1.2 820,826 - -0.1 820,826 - +0.5 820,926 - ybhN b0788 0.17 +1.5 821,003 + ybhN b0788 0.25 +0.9 821,004 - ybhN b0788 0.25 +0.7 821,158 - ybhN b0788 0.41 +1.2 821,314 - ybhN b0788 0.57 +1.3 821,329 + ybhN b0788 0.59 +0.4 821,333 - ybhN b0788 0.59 -1.2 821,435 - ybhN b0788 0.70 +0.3 821,615 - ybhN b0788 0.89 +2.1 821,666 - -1.1 821,850 - ybhO b0789 0.10 -0.8 821,898 - ybhO b0789 0.14 -1.6 821,907 - ybhO b0789 0.15 +1.2 822,100 + ybhO b0789 0.31 +3.9 822,100 + ybhO b0789 0.31 +1.5 822,155 - ybhO b0789 0.35 +0.4 822,188 - ybhO b0789 0.38 +0.7 822,188 - ybhO b0789 0.38 +1.0 822,273 + ybhO b0789 0.44 +1.5 822,328 - ybhO b0789 0.49 -2.5 822,328 - ybhO b0789 0.49 -0.7 822,553 + ybhO b0789 0.67 +1.7 822,553 + ybhO b0789 0.67 +2.9 822,780 + ybhO b0789 0.85 +0.9 822,780 + ybhO b0789 0.85 +0.6 822,849 + -0.9 822,891 - -2.8 823,224 + ybhP b0790 0.35 -0.0 823,224 + ybhP b0790 0.35 -1.2 823,296 - ybhP b0790 0.44 -1.5 823,296 - ybhP b0790 0.44 -1.6 823,302 - ybhP b0790 0.45 +0.6 823,331 + ybhP b0790 0.49 -0.9 823,346 + ybhP b0790 0.51 -0.0 823,405 + ybhP b0790 0.59 -0.8 823,434 - ybhP b0790 0.62 +0.7 823,454 - ybhP b0790 0.65 +0.6 823,604 - ybhP b0790 0.85 +0.3 823,653 - -0.6 823,850 + +1.0 823,893 - +0.6 823,947 + ybhQ b0791 0.23 -0.8 823,961 - ybhQ b0791 0.26 -0.6
Or see this region's nucleotide sequence