Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybeA and ybeB are separated by 3 nucleotides ybeB and cobC are separated by 259 nucleotides cobC and nadD are separated by 23 nucleotides nadD and holA are separated by 1 nucleotides
b0636: ybeA - hypothetical protein (NCBI), at 667,471 to 667,938
ybeA
b0637: ybeB - orf, hypothetical protein (VIMSS), at 667,942 to 668,259
ybeB
b0638: cobC - predicted alpha-ribazole-5'-P phosphatase (NCBI), at 668,519 to 669,130
cobC
b0639: nadD - nicotinic acid mononucleotide adenyltransferase (NCBI), at 669,154 to 669,795
nadD
b0640: holA - DNA polymerase III subunit delta (NCBI), at 669,797 to 670,828
holA
Position (kb) 668
669
670 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 667.524 kb on - strand, within ybeA at 667.524 kb on - strand, within ybeA at 667.588 kb on - strand, within ybeA at 667.590 kb on - strand, within ybeA at 667.665 kb on - strand, within ybeA at 667.665 kb on - strand, within ybeA at 667.703 kb on - strand, within ybeA at 667.703 kb on - strand, within ybeA at 667.762 kb on - strand, within ybeA at 667.762 kb on - strand, within ybeA at 667.852 kb on - strand, within ybeA at 667.852 kb on - strand, within ybeA at 668.424 kb on + strand at 668.466 kb on - strand at 668.466 kb on - strand at 668.601 kb on - strand, within cobC at 668.672 kb on + strand, within cobC at 668.680 kb on - strand, within cobC at 668.733 kb on + strand, within cobC at 668.733 kb on + strand, within cobC at 668.796 kb on - strand, within cobC at 668.796 kb on - strand, within cobC at 668.930 kb on + strand, within cobC at 668.948 kb on + strand, within cobC at 668.948 kb on + strand, within cobC at 668.992 kb on + strand, within cobC at 669.031 kb on + strand, within cobC at 669.048 kb on + strand, within cobC at 669.057 kb on - strand, within cobC at 669.057 kb on - strand, within cobC at 669.095 kb on - strand at 669.153 kb on - strand at 669.158 kb on - strand at 669.158 kb on - strand at 669.179 kb on + strand at 669.187 kb on - strand at 669.852 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 667,524 - ybeA b0636 0.11 +0.4 667,524 - ybeA b0636 0.11 +0.9 667,588 - ybeA b0636 0.25 +0.9 667,590 - ybeA b0636 0.25 -1.0 667,665 - ybeA b0636 0.41 +0.9 667,665 - ybeA b0636 0.41 -0.1 667,703 - ybeA b0636 0.50 +0.4 667,703 - ybeA b0636 0.50 -2.3 667,762 - ybeA b0636 0.62 +2.0 667,762 - ybeA b0636 0.62 +1.9 667,852 - ybeA b0636 0.81 +0.2 667,852 - ybeA b0636 0.81 -0.1 668,424 + -2.1 668,466 - -0.5 668,466 - -0.4 668,601 - cobC b0638 0.13 +0.1 668,672 + cobC b0638 0.25 -0.8 668,680 - cobC b0638 0.26 -1.3 668,733 + cobC b0638 0.35 +0.6 668,733 + cobC b0638 0.35 -0.4 668,796 - cobC b0638 0.45 -2.2 668,796 - cobC b0638 0.45 +0.4 668,930 + cobC b0638 0.67 -1.0 668,948 + cobC b0638 0.70 +3.5 668,948 + cobC b0638 0.70 +0.5 668,992 + cobC b0638 0.77 +0.3 669,031 + cobC b0638 0.84 -0.4 669,048 + cobC b0638 0.86 -0.5 669,057 - cobC b0638 0.88 -0.6 669,057 - cobC b0638 0.88 +1.1 669,095 - +0.5 669,153 - +1.1 669,158 - +1.2 669,158 - -0.9 669,179 + -0.4 669,187 - -1.1 669,852 + +0.5
Or see this region's nucleotide sequence