Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybaT and cueR are separated by 124 nucleotides cueR and ybbJ are separated by 0 nucleotides ybbJ and ybbK overlap by 4 nucleotides
b0486: ybaT - predicted transporter (NCBI), at 511,800 to 513,092
ybaT
b0487: cueR - DNA-binding transcriptional activator of copper-responsive regulon genes (NCBI), at 513,217 to 513,624
cueR
b0488: ybbJ - conserved inner membrane protein (RefSeq), at 513,625 to 514,083
ybbJ
b0489: ybbK - predicted protease, membrane anchored (NCBI), at 514,080 to 514,997
ybbK
Position (kb) 513
514 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 512.239 kb on - strand, within ybaT at 512.413 kb on - strand, within ybaT at 512.414 kb on - strand, within ybaT at 512.514 kb on - strand, within ybaT at 512.567 kb on - strand, within ybaT at 512.604 kb on + strand, within ybaT at 512.771 kb on + strand, within ybaT at 512.830 kb on + strand, within ybaT at 512.830 kb on + strand, within ybaT at 512.846 kb on - strand, within ybaT at 512.886 kb on + strand, within ybaT at 512.890 kb on + strand, within ybaT at 512.953 kb on + strand, within ybaT at 512.958 kb on + strand, within ybaT at 512.958 kb on + strand, within ybaT at 512.991 kb on + strand at 512.998 kb on + strand at 512.998 kb on + strand at 513.013 kb on - strand at 513.013 kb on - strand at 513.039 kb on + strand at 513.039 kb on + strand at 513.057 kb on - strand at 513.057 kb on - strand at 513.057 kb on - strand at 513.077 kb on + strand at 513.077 kb on + strand at 513.095 kb on - strand at 513.107 kb on + strand at 513.109 kb on + strand at 513.182 kb on + strand at 513.205 kb on - strand at 513.287 kb on - strand, within cueR at 513.295 kb on - strand, within cueR at 513.431 kb on - strand, within cueR at 513.480 kb on + strand, within cueR at 513.537 kb on - strand, within cueR at 513.537 kb on - strand, within cueR at 513.700 kb on - strand, within ybbJ at 513.728 kb on - strand, within ybbJ at 513.742 kb on + strand, within ybbJ at 513.742 kb on + strand, within ybbJ at 513.949 kb on - strand, within ybbJ at 513.949 kb on - strand, within ybbJ at 513.958 kb on + strand, within ybbJ at 513.958 kb on + strand, within ybbJ at 513.977 kb on + strand, within ybbJ at 514.116 kb on - strand at 514.116 kb on - strand at 514.118 kb on - strand at 514.118 kb on - strand at 514.129 kb on - strand at 514.133 kb on - strand at 514.133 kb on - strand at 514.142 kb on + strand at 514.142 kb on + strand at 514.185 kb on + strand, within ybbK at 514.473 kb on - strand, within ybbK at 514.546 kb on - strand, within ybbK
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 512,239 - ybaT b0486 0.34 +1.9 512,413 - ybaT b0486 0.47 -3.0 512,414 - ybaT b0486 0.47 +0.3 512,514 - ybaT b0486 0.55 +0.4 512,567 - ybaT b0486 0.59 -1.2 512,604 + ybaT b0486 0.62 -1.1 512,771 + ybaT b0486 0.75 -1.8 512,830 + ybaT b0486 0.80 -0.3 512,830 + ybaT b0486 0.80 +1.3 512,846 - ybaT b0486 0.81 -1.8 512,886 + ybaT b0486 0.84 +1.6 512,890 + ybaT b0486 0.84 +0.9 512,953 + ybaT b0486 0.89 +0.4 512,958 + ybaT b0486 0.90 +0.7 512,958 + ybaT b0486 0.90 -0.7 512,991 + -2.3 512,998 + -0.1 512,998 + -0.1 513,013 - -2.4 513,013 - -0.3 513,039 + +5.7 513,039 + +0.6 513,057 - +0.5 513,057 - +0.4 513,057 - -2.0 513,077 + +0.8 513,077 + -0.1 513,095 - -0.3 513,107 + -0.6 513,109 + +1.7 513,182 + +0.5 513,205 - +2.2 513,287 - cueR b0487 0.17 +1.8 513,295 - cueR b0487 0.19 +2.1 513,431 - cueR b0487 0.52 +1.5 513,480 + cueR b0487 0.64 -0.8 513,537 - cueR b0487 0.78 +1.3 513,537 - cueR b0487 0.78 +0.0 513,700 - ybbJ b0488 0.16 +0.5 513,728 - ybbJ b0488 0.22 +0.6 513,742 + ybbJ b0488 0.25 -0.2 513,742 + ybbJ b0488 0.25 +0.0 513,949 - ybbJ b0488 0.71 -0.3 513,949 - ybbJ b0488 0.71 +1.1 513,958 + ybbJ b0488 0.73 +1.1 513,958 + ybbJ b0488 0.73 -0.8 513,977 + ybbJ b0488 0.77 -0.4 514,116 - -0.2 514,116 - +1.0 514,118 - +0.5 514,118 - -1.4 514,129 - +0.2 514,133 - -0.2 514,133 - +0.1 514,142 + -1.3 514,142 + +1.4 514,185 + ybbK b0489 0.11 +0.8 514,473 - ybbK b0489 0.43 +0.6 514,546 - ybbK b0489 0.51 +0.2
Or see this region's nucleotide sequence