Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phoA and psiF are separated by 118 nucleotides psiF and yaiC are separated by 101 nucleotides
b0383: phoA - alkaline phosphatase (VIMSS), at 400,971 to 402,386
phoA
b0384: psiF - induced by phosphate starvation (VIMSS), at 402,505 to 402,825
psiF
b0385: yaiC - predicted diguanylate cyclase (NCBI), at 402,927 to 404,042
yaiC
Position (kb) 402
403 Strain fitness (log2 ratio) -2
-1
0
1
2 at 401.616 kb on + strand, within phoA at 401.621 kb on + strand, within phoA at 401.653 kb on - strand, within phoA at 401.800 kb on + strand, within phoA at 401.800 kb on + strand, within phoA at 401.808 kb on - strand, within phoA at 401.808 kb on - strand, within phoA at 401.830 kb on - strand, within phoA at 401.839 kb on + strand, within phoA at 401.888 kb on + strand, within phoA at 401.930 kb on - strand, within phoA at 402.081 kb on + strand, within phoA at 402.081 kb on + strand, within phoA at 402.086 kb on - strand, within phoA at 402.144 kb on - strand, within phoA at 402.183 kb on + strand, within phoA at 402.218 kb on - strand, within phoA at 402.218 kb on - strand, within phoA at 402.246 kb on - strand at 402.291 kb on + strand at 402.291 kb on + strand at 402.350 kb on + strand at 402.605 kb on - strand, within psiF at 402.605 kb on - strand, within psiF at 402.689 kb on - strand, within psiF at 402.689 kb on - strand, within psiF at 402.733 kb on - strand, within psiF at 402.733 kb on - strand, within psiF at 402.826 kb on - strand at 402.827 kb on + strand at 402.835 kb on - strand at 402.840 kb on + strand at 402.873 kb on - strand at 402.873 kb on - strand at 403.026 kb on + strand at 403.050 kb on + strand, within yaiC at 403.050 kb on + strand, within yaiC at 403.058 kb on - strand, within yaiC at 403.063 kb on + strand, within yaiC at 403.130 kb on + strand, within yaiC at 403.291 kb on + strand, within yaiC at 403.558 kb on - strand, within yaiC at 403.558 kb on - strand, within yaiC at 403.574 kb on - strand, within yaiC at 403.574 kb on - strand, within yaiC at 403.600 kb on - strand, within yaiC at 403.600 kb on - strand, within yaiC
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 401,616 + phoA b0383 0.46 -0.4 401,621 + phoA b0383 0.46 -2.3 401,653 - phoA b0383 0.48 -0.6 401,800 + phoA b0383 0.59 +0.6 401,800 + phoA b0383 0.59 -0.6 401,808 - phoA b0383 0.59 +1.9 401,808 - phoA b0383 0.59 -1.2 401,830 - phoA b0383 0.61 +0.4 401,839 + phoA b0383 0.61 +0.3 401,888 + phoA b0383 0.65 -0.1 401,930 - phoA b0383 0.68 +0.1 402,081 + phoA b0383 0.78 +0.9 402,081 + phoA b0383 0.78 -1.0 402,086 - phoA b0383 0.79 +1.8 402,144 - phoA b0383 0.83 +0.7 402,183 + phoA b0383 0.86 +0.6 402,218 - phoA b0383 0.88 -2.1 402,218 - phoA b0383 0.88 -0.9 402,246 - +1.9 402,291 + -1.2 402,291 + -2.1 402,350 + +0.2 402,605 - psiF b0384 0.31 -0.3 402,605 - psiF b0384 0.31 +0.0 402,689 - psiF b0384 0.57 -1.9 402,689 - psiF b0384 0.57 +0.6 402,733 - psiF b0384 0.71 -1.1 402,733 - psiF b0384 0.71 -0.6 402,826 - +0.3 402,827 + +0.9 402,835 - +1.5 402,840 + -2.0 402,873 - +0.7 402,873 - -0.1 403,026 + +0.5 403,050 + yaiC b0385 0.11 -0.1 403,050 + yaiC b0385 0.11 -1.7 403,058 - yaiC b0385 0.12 -0.5 403,063 + yaiC b0385 0.12 -1.9 403,130 + yaiC b0385 0.18 -0.3 403,291 + yaiC b0385 0.33 -0.5 403,558 - yaiC b0385 0.57 +0.6 403,558 - yaiC b0385 0.57 +0.5 403,574 - yaiC b0385 0.58 -1.2 403,574 - yaiC b0385 0.58 +2.2 403,600 - yaiC b0385 0.60 -0.9 403,600 - yaiC b0385 0.60 -1.1
Or see this region's nucleotide sequence