Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafZ and ykfA are separated by 91 nucleotides ykfA and perR are separated by 328 nucleotides
b0252: yafZ - orf, hypothetical protein (VIMSS), at 266,408 to 267,229
yafZ
b0253: ykfA - putative GTP-binding protein (VIMSS), at 267,321 to 268,184
ykfA
b0254: perR - CP4-6 prophage; predicted DNA-binding transcriptional regulator (NCBI), at 268,513 to 269,406
perR
Position (kb)
267
268
269 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 266.353 kb on - strand at 266.433 kb on + strand at 266.436 kb on - strand at 266.449 kb on + strand at 266.464 kb on - strand at 266.569 kb on - strand, within yafZ at 266.668 kb on - strand, within yafZ at 266.773 kb on - strand, within yafZ at 266.810 kb on - strand, within yafZ at 266.946 kb on - strand, within yafZ at 267.028 kb on - strand, within yafZ at 267.028 kb on - strand, within yafZ at 267.062 kb on - strand, within yafZ at 267.062 kb on - strand, within yafZ at 267.093 kb on + strand, within yafZ at 267.122 kb on + strand, within yafZ at 267.137 kb on + strand, within yafZ at 267.137 kb on + strand, within yafZ at 267.168 kb on - strand at 267.229 kb on + strand at 267.229 kb on + strand at 267.289 kb on + strand at 267.289 kb on + strand at 267.317 kb on + strand at 267.320 kb on - strand at 267.351 kb on - strand at 267.351 kb on - strand at 267.437 kb on - strand, within ykfA at 267.580 kb on - strand, within ykfA at 267.604 kb on + strand, within ykfA at 267.607 kb on - strand, within ykfA at 267.607 kb on - strand, within ykfA at 267.617 kb on - strand, within ykfA at 267.622 kb on + strand, within ykfA at 267.622 kb on + strand, within ykfA at 267.622 kb on + strand, within ykfA at 267.687 kb on - strand, within ykfA at 267.715 kb on - strand, within ykfA at 267.716 kb on + strand, within ykfA at 267.720 kb on - strand, within ykfA at 267.726 kb on + strand, within ykfA at 267.734 kb on - strand, within ykfA at 267.758 kb on - strand, within ykfA at 267.771 kb on - strand, within ykfA at 267.828 kb on + strand, within ykfA at 267.838 kb on - strand, within ykfA at 267.838 kb on - strand, within ykfA at 267.864 kb on - strand, within ykfA at 267.869 kb on - strand, within ykfA at 267.879 kb on - strand, within ykfA at 267.879 kb on - strand, within ykfA at 267.879 kb on - strand, within ykfA at 267.879 kb on - strand, within ykfA at 267.909 kb on + strand, within ykfA at 267.933 kb on - strand, within ykfA at 267.978 kb on - strand, within ykfA at 267.978 kb on - strand, within ykfA at 268.000 kb on + strand, within ykfA at 268.120 kb on + strand at 268.302 kb on - strand at 268.305 kb on + strand at 268.310 kb on - strand at 268.310 kb on - strand at 268.316 kb on - strand at 268.326 kb on - strand at 268.373 kb on + strand at 268.384 kb on + strand at 268.384 kb on + strand at 268.385 kb on + strand at 268.393 kb on - strand at 268.494 kb on - strand at 268.494 kb on - strand at 268.538 kb on - strand at 268.584 kb on - strand at 268.591 kb on - strand at 268.591 kb on - strand at 268.623 kb on - strand, within perR at 268.645 kb on - strand, within perR at 268.657 kb on - strand, within perR at 268.809 kb on - strand, within perR at 268.853 kb on + strand, within perR at 268.873 kb on + strand, within perR at 268.873 kb on + strand, within perR at 268.928 kb on + strand, within perR at 268.967 kb on - strand, within perR at 268.967 kb on - strand, within perR at 268.971 kb on - strand, within perR at 268.971 kb on - strand, within perR at 268.993 kb on - strand, within perR at 269.108 kb on + strand, within perR at 269.108 kb on + strand, within perR at 269.116 kb on - strand, within perR at 269.116 kb on - strand, within perR at 269.116 kb on - strand, within perR at 269.131 kb on + strand, within perR at 269.157 kb on - strand, within perR
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 266,353 - +0.3 266,433 + -2.5 266,436 - -3.0 266,449 + +0.7 266,464 - +1.9 266,569 - yafZ b0252 0.20 +1.6 266,668 - yafZ b0252 0.32 -0.2 266,773 - yafZ b0252 0.44 +0.3 266,810 - yafZ b0252 0.49 +0.1 266,946 - yafZ b0252 0.65 +1.4 267,028 - yafZ b0252 0.75 +0.4 267,028 - yafZ b0252 0.75 -3.0 267,062 - yafZ b0252 0.80 -1.6 267,062 - yafZ b0252 0.80 -0.1 267,093 + yafZ b0252 0.83 +0.1 267,122 + yafZ b0252 0.87 +1.1 267,137 + yafZ b0252 0.89 +1.4 267,137 + yafZ b0252 0.89 +2.7 267,168 - -0.8 267,229 + -1.1 267,229 + -3.0 267,289 + +2.7 267,289 + -0.5 267,317 + -2.5 267,320 - +1.6 267,351 - +0.5 267,351 - -1.4 267,437 - ykfA b0253 0.13 -1.2 267,580 - ykfA b0253 0.30 -1.0 267,604 + ykfA b0253 0.33 +0.3 267,607 - ykfA b0253 0.33 +0.9 267,607 - ykfA b0253 0.33 +0.6 267,617 - ykfA b0253 0.34 +2.4 267,622 + ykfA b0253 0.35 -0.5 267,622 + ykfA b0253 0.35 -0.1 267,622 + ykfA b0253 0.35 +1.4 267,687 - ykfA b0253 0.42 -0.6 267,715 - ykfA b0253 0.46 -2.8 267,716 + ykfA b0253 0.46 +0.7 267,720 - ykfA b0253 0.46 -0.3 267,726 + ykfA b0253 0.47 -1.3 267,734 - ykfA b0253 0.48 +2.0 267,758 - ykfA b0253 0.51 -1.7 267,771 - ykfA b0253 0.52 -0.0 267,828 + ykfA b0253 0.59 +3.9 267,838 - ykfA b0253 0.60 +1.6 267,838 - ykfA b0253 0.60 +0.4 267,864 - ykfA b0253 0.63 -0.4 267,869 - ykfA b0253 0.63 +1.1 267,879 - ykfA b0253 0.65 -0.6 267,879 - ykfA b0253 0.65 +1.0 267,879 - ykfA b0253 0.65 +2.2 267,879 - ykfA b0253 0.65 -0.8 267,909 + ykfA b0253 0.68 +0.3 267,933 - ykfA b0253 0.71 +1.2 267,978 - ykfA b0253 0.76 -1.5 267,978 - ykfA b0253 0.76 -2.1 268,000 + ykfA b0253 0.79 +1.1 268,120 + -0.7 268,302 - -1.5 268,305 + +0.9 268,310 - +0.9 268,310 - -1.0 268,316 - +1.2 268,326 - -0.7 268,373 + +0.2 268,384 + +2.7 268,384 + -1.9 268,385 + +0.1 268,393 - -0.6 268,494 - +0.6 268,494 - -0.8 268,538 - +0.5 268,584 - -0.1 268,591 - -1.0 268,591 - -0.1 268,623 - perR b0254 0.12 -2.1 268,645 - perR b0254 0.15 -1.7 268,657 - perR b0254 0.16 +1.5 268,809 - perR b0254 0.33 -0.3 268,853 + perR b0254 0.38 +1.0 268,873 + perR b0254 0.40 +0.8 268,873 + perR b0254 0.40 +0.9 268,928 + perR b0254 0.46 +0.0 268,967 - perR b0254 0.51 +0.6 268,967 - perR b0254 0.51 +0.4 268,971 - perR b0254 0.51 +2.1 268,971 - perR b0254 0.51 -0.1 268,993 - perR b0254 0.54 -3.7 269,108 + perR b0254 0.67 +0.9 269,108 + perR b0254 0.67 -0.5 269,116 - perR b0254 0.67 +0.1 269,116 - perR b0254 0.67 -0.1 269,116 - perR b0254 0.67 -1.5 269,131 + perR b0254 0.69 +1.3 269,157 - perR b0254 0.72 -2.1
Or see this region's nucleotide sequence