Strain Fitness in Escherichia coli BW25113 around b0102

Experiment: 3-methyl-1-butanol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsecA and mutT are separated by 59 nucleotidesmutT and yacG are separated by 215 nucleotidesyacG and yacF are separated by 9 nucleotidesyacF and coaE overlap by 1 nucleotidescoaE and guaC are separated by 224 nucleotides b0098: secA - translocase (NCBI), at 108,279 to 110,984 secA b0099: mutT - nucleoside triphosphate pyrophosphohydrolase, marked preference for dGTP (NCBI), at 111,044 to 111,433 mutT b0101: yacG - zinc-binding protein (NCBI), at 111,649 to 111,846 yacG b0102: yacF - hypothetical protein (NCBI), at 111,856 to 112,599 yacF b0103: coaE - dephospho-CoA kinase (NCBI), at 112,599 to 113,219 coaE b0104: guaC - guanosine 5'-monophosphate oxidoreductase (NCBI), at 113,444 to 114,487 guaC Position (kb) 111 112 113Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 111.014 kb on + strandat 111.266 kb on + strand, within mutTat 111.276 kb on + strand, within mutTat 111.279 kb on + strand, within mutTat 111.328 kb on + strand, within mutTat 111.336 kb on - strand, within mutTat 111.426 kb on - strandat 111.426 kb on - strandat 111.504 kb on - strandat 111.504 kb on - strandat 111.616 kb on - strandat 111.792 kb on - strand, within yacGat 111.795 kb on + strand, within yacGat 111.795 kb on + strand, within yacGat 111.823 kb on + strand, within yacGat 111.828 kb on + strandat 111.828 kb on + strandat 111.836 kb on - strandat 111.836 kb on - strandat 111.886 kb on + strandat 111.995 kb on - strand, within yacFat 111.997 kb on + strand, within yacFat 112.005 kb on - strandat 112.005 kb on - strand, within yacFat 112.089 kb on - strand, within yacFat 112.089 kb on - strand, within yacFat 112.155 kb on + strand, within yacFat 112.188 kb on - strand, within yacFat 112.347 kb on + strand, within yacFat 112.347 kb on + strand, within yacFat 112.347 kb on + strand, within yacFat 112.366 kb on + strand, within yacFat 112.397 kb on - strand, within yacFat 112.553 kb on - strandat 112.553 kb on - strandat 113.326 kb on + strandat 113.357 kb on + strandat 113.367 kb on + strandat 113.375 kb on - strandat 113.375 kb on - strandat 113.379 kb on + strandat 113.379 kb on + strandat 113.384 kb on + strandat 113.384 kb on + strandat 113.400 kb on + strandat 113.446 kb on - strandat 113.475 kb on + strandat 113.480 kb on - strandat 113.503 kb on - strandat 113.510 kb on + strandat 113.510 kb on + strandat 113.564 kb on + strand, within guaC

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol%
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111,014 + -0.2
111,266 + mutT b0099 0.57 +0.6
111,276 + mutT b0099 0.59 -1.1
111,279 + mutT b0099 0.60 +0.9
111,328 + mutT b0099 0.73 -0.1
111,336 - mutT b0099 0.75 +0.5
111,426 - -0.6
111,426 - +0.8
111,504 - -0.5
111,504 - +0.3
111,616 - -0.1
111,792 - yacG b0101 0.72 +1.4
111,795 + yacG b0101 0.74 -1.5
111,795 + yacG b0101 0.74 +1.5
111,823 + yacG b0101 0.88 +1.9
111,828 + +0.4
111,828 + +1.6
111,836 - +0.1
111,836 - +0.6
111,886 + +2.4
111,995 - yacF b0102 0.19 -0.4
111,997 + yacF b0102 0.19 +1.7
112,005 - +1.1
112,005 - yacF b0102 0.20 +3.7
112,089 - yacF b0102 0.31 -2.1
112,089 - yacF b0102 0.31 -1.2
112,155 + yacF b0102 0.40 +4.6
112,188 - yacF b0102 0.45 +2.0
112,347 + yacF b0102 0.66 +1.3
112,347 + yacF b0102 0.66 +3.5
112,347 + yacF b0102 0.66 +3.5
112,366 + yacF b0102 0.69 -1.1
112,397 - yacF b0102 0.73 -2.6
112,553 - +2.6
112,553 - -3.1
113,326 + +2.2
113,357 + -1.7
113,367 + -0.4
113,375 - -2.9
113,375 - +0.4
113,379 + -1.6
113,379 + +1.0
113,384 + +0.7
113,384 + -2.9
113,400 + +0.8
113,446 - +0.1
113,475 + -2.7
113,480 - -0.3
113,503 - -0.3
113,510 + +1.7
113,510 + +0.3
113,564 + guaC b0104 0.11 -1.1

Or see this region's nucleotide sequence