Strain Fitness in Escherichia coli BW25113 around b0088

Experiment: 3-methyl-1-butanol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmraY and murD are separated by 2 nucleotidesmurD and ftsW overlap by 1 nucleotides b0087: mraY - phospho-N-acetylmuramoyl-pentapeptide-transferase (NCBI), at 96,002 to 97,084 mraY b0088: murD - UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI), at 97,087 to 98,403 murD b0089: ftsW - integral membrane protein involved in stabilizing FstZ ring during cell division (NCBI), at 98,403 to 99,647 ftsW Position (kb) 97 98 99Strain fitness (log2 ratio) -1 0 1 2at 97.078 kb on + strandat 97.329 kb on + strand, within murDat 97.380 kb on + strand, within murDat 97.787 kb on + strand, within murDat 98.441 kb on + strandat 98.490 kb on + strandat 98.502 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol%
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97,078 + +1.0
97,329 + murD b0088 0.18 +2.1
97,380 + murD b0088 0.22 +2.4
97,787 + murD b0088 0.53 -1.7
98,441 + +0.4
98,490 + +0.7
98,502 + -0.2

Or see this region's nucleotide sequence