Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fixB and fixC are separated by 50 nucleotides fixC and fixX overlap by 4 nucleotides fixX and yaaU are separated by 56 nucleotides
b0042: fixB - predicted electron transfer flavoprotein, NAD/FAD-binding domain and ETFP adenine nucleotide-binding domain-like (NCBI), at 43,188 to 44,129
fixB
b0043: fixC - predicted oxidoreductase with FAD/NAD(P)-binding domain (NCBI), at 44,180 to 45,466
fixC
b0044: fixX - predicted 4Fe-4S ferredoxin-type protein (NCBI), at 45,463 to 45,750
fixX
b0045: yaaU - predicted transporter (NCBI), at 45,807 to 47,138
yaaU
Position (kb) 44
45
46 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 43.236 kb on - strand at 43.236 kb on - strand at 43.237 kb on + strand at 43.237 kb on + strand at 43.270 kb on - strand at 43.318 kb on - strand, within fixB at 43.432 kb on + strand, within fixB at 43.506 kb on + strand, within fixB at 43.531 kb on + strand, within fixB at 43.539 kb on - strand, within fixB at 43.574 kb on - strand, within fixB at 43.610 kb on + strand, within fixB at 43.630 kb on - strand, within fixB at 43.630 kb on - strand, within fixB at 43.682 kb on - strand, within fixB at 43.692 kb on + strand, within fixB at 43.740 kb on + strand, within fixB at 43.740 kb on + strand, within fixB at 43.835 kb on + strand, within fixB at 43.835 kb on + strand, within fixB at 43.835 kb on - strand, within fixB at 43.838 kb on + strand, within fixB at 43.867 kb on + strand, within fixB at 43.867 kb on + strand, within fixB at 43.871 kb on - strand, within fixB at 43.924 kb on + strand, within fixB at 43.952 kb on + strand, within fixB at 43.970 kb on - strand, within fixB at 44.076 kb on + strand at 44.158 kb on - strand at 44.176 kb on + strand at 44.214 kb on + strand at 44.214 kb on + strand at 44.214 kb on + strand at 44.222 kb on - strand at 44.243 kb on - strand at 44.326 kb on + strand, within fixC at 44.335 kb on - strand, within fixC at 44.335 kb on - strand, within fixC at 44.398 kb on - strand, within fixC at 44.581 kb on - strand, within fixC at 44.622 kb on - strand, within fixC at 44.622 kb on - strand, within fixC at 44.649 kb on + strand, within fixC at 44.675 kb on - strand, within fixC at 44.676 kb on - strand, within fixC at 44.765 kb on + strand, within fixC at 44.857 kb on - strand, within fixC at 44.862 kb on - strand, within fixC at 44.906 kb on - strand, within fixC at 45.034 kb on + strand, within fixC at 45.034 kb on + strand, within fixC at 45.044 kb on + strand, within fixC at 45.044 kb on + strand, within fixC at 45.232 kb on + strand, within fixC at 45.241 kb on - strand, within fixC at 45.323 kb on - strand, within fixC at 45.339 kb on + strand at 45.339 kb on + strand at 45.340 kb on - strand at 45.426 kb on + strand at 45.546 kb on - strand, within fixX at 45.610 kb on + strand, within fixX at 45.700 kb on - strand, within fixX at 45.704 kb on + strand, within fixX at 45.704 kb on + strand, within fixX at 45.844 kb on + strand at 45.880 kb on - strand at 45.924 kb on + strand at 46.113 kb on + strand, within yaaU at 46.113 kb on + strand, within yaaU at 46.142 kb on - strand, within yaaU at 46.144 kb on - strand, within yaaU at 46.176 kb on - strand, within yaaU at 46.176 kb on - strand, within yaaU at 46.240 kb on + strand, within yaaU at 46.284 kb on - strand, within yaaU at 46.329 kb on + strand, within yaaU at 46.329 kb on + strand, within yaaU at 46.463 kb on + strand, within yaaU at 46.463 kb on + strand, within yaaU
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 43,236 - -1.7 43,236 - +0.2 43,237 + +0.8 43,237 + +0.2 43,270 - +1.0 43,318 - fixB b0042 0.14 +1.2 43,432 + fixB b0042 0.26 -1.4 43,506 + fixB b0042 0.34 +0.5 43,531 + fixB b0042 0.36 -1.1 43,539 - fixB b0042 0.37 +1.2 43,574 - fixB b0042 0.41 -1.5 43,610 + fixB b0042 0.45 -0.8 43,630 - fixB b0042 0.47 -0.2 43,630 - fixB b0042 0.47 -0.7 43,682 - fixB b0042 0.52 -0.6 43,692 + fixB b0042 0.54 +0.5 43,740 + fixB b0042 0.59 -1.5 43,740 + fixB b0042 0.59 +0.3 43,835 + fixB b0042 0.69 +0.5 43,835 + fixB b0042 0.69 +2.0 43,835 - fixB b0042 0.69 -1.0 43,838 + fixB b0042 0.69 -1.3 43,867 + fixB b0042 0.72 +0.2 43,867 + fixB b0042 0.72 -0.3 43,871 - fixB b0042 0.73 +1.2 43,924 + fixB b0042 0.78 -1.7 43,952 + fixB b0042 0.81 +0.9 43,970 - fixB b0042 0.83 -1.0 44,076 + +0.3 44,158 - +1.3 44,176 + -0.7 44,214 + -0.6 44,214 + +0.8 44,214 + +0.5 44,222 - +1.0 44,243 - +0.0 44,326 + fixC b0043 0.11 -0.5 44,335 - fixC b0043 0.12 +0.3 44,335 - fixC b0043 0.12 -1.1 44,398 - fixC b0043 0.17 +3.4 44,581 - fixC b0043 0.31 -0.4 44,622 - fixC b0043 0.34 -2.1 44,622 - fixC b0043 0.34 -1.5 44,649 + fixC b0043 0.36 -0.3 44,675 - fixC b0043 0.38 -3.5 44,676 - fixC b0043 0.39 +0.5 44,765 + fixC b0043 0.45 +0.5 44,857 - fixC b0043 0.53 -2.5 44,862 - fixC b0043 0.53 +1.4 44,906 - fixC b0043 0.56 +0.9 45,034 + fixC b0043 0.66 -2.0 45,034 + fixC b0043 0.66 -1.0 45,044 + fixC b0043 0.67 -0.4 45,044 + fixC b0043 0.67 -1.0 45,232 + fixC b0043 0.82 -0.6 45,241 - fixC b0043 0.82 -1.5 45,323 - fixC b0043 0.89 -0.8 45,339 + -0.3 45,339 + +1.6 45,340 - +1.3 45,426 + -0.9 45,546 - fixX b0044 0.29 -0.4 45,610 + fixX b0044 0.51 +0.2 45,700 - fixX b0044 0.82 -0.1 45,704 + fixX b0044 0.84 -0.6 45,704 + fixX b0044 0.84 -0.5 45,844 + +1.0 45,880 - -0.2 45,924 + +0.2 46,113 + yaaU b0045 0.23 -1.6 46,113 + yaaU b0045 0.23 -0.7 46,142 - yaaU b0045 0.25 +1.4 46,144 - yaaU b0045 0.25 +1.9 46,176 - yaaU b0045 0.28 -0.7 46,176 - yaaU b0045 0.28 -0.2 46,240 + yaaU b0045 0.33 +0.0 46,284 - yaaU b0045 0.36 -0.9 46,329 + yaaU b0045 0.39 +0.4 46,329 + yaaU b0045 0.39 -1.1 46,463 + yaaU b0045 0.49 +1.6 46,463 + yaaU b0045 0.49 +0.0
Or see this region's nucleotide sequence