Strain Fitness in Pseudomonas putida KT2440 around PP_3744

Experiment: 3-methyl-3-butenol (commercial) 0.25 vol%

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-3-butenol (commercial) 0.25 vol%
4,272,100	+		PP_3742	0.48	-0.1
4,272,101	-		PP_3742	0.48	-0.8
4,272,103	+		PP_3742	0.48	+0.1
4,272,103	+		PP_3742	0.48	-0.0
4,272,104	-		PP_3742	0.49	+0.9
4,272,104	-		PP_3742	0.49	-0.1
4,272,104	-		PP_3742	0.49	-0.9
4,272,104	-		PP_3742	0.49	-0.7
4,272,116	+		PP_3742	0.51	+0.3
4,272,116	+		PP_3742	0.51	-0.0
4,272,380	-				+0.1
4,272,380	-				-0.2
4,272,380	-				-0.4
4,272,633	+				-0.3
4,272,633	+				+0.1
4,272,655	+				-0.5
4,272,656	-				-0.8
4,272,672	-				+0.2
4,272,672	-				+0.7
4,272,692	-				-0.3
4,272,717	+				+0.3
4,272,718	-				+0.1
4,272,718	-				-0.3
4,272,806	-		PP_3743	0.31	+1.1
4,272,822	-		PP_3743	0.36	+0.8
4,272,911	+		PP_3743	0.64	-1.3
4,272,911	+		PP_3743	0.64	+0.6
4,272,911	+		PP_3743	0.64	+0.0
4,272,912	-		PP_3743	0.64	+0.9
4,272,912	-		PP_3743	0.64	-0.5
4,272,912	-		PP_3743	0.64	+0.1
4,272,912	-		PP_3743	0.64	-0.5
4,273,073	+				+1.1
4,273,074	-				-0.3
4,273,102	+				+0.5
4,273,103	-				-0.8
4,273,163	+				-0.9
4,273,163	+				+0.5
4,273,174	+		PP_3744	0.11	+1.0
4,273,174	+		PP_3744	0.11	+0.1
4,273,174	+		PP_3744	0.11	+0.6
4,273,175	-		PP_3744	0.11	+1.0
4,273,175	-		PP_3744	0.11	+0.2
4,273,175	-		PP_3744	0.11	+0.3
4,273,282	-		PP_3744	0.25	+0.1
4,273,343	-		PP_3744	0.33	-1.6
4,273,361	-		PP_3744	0.35	+0.4
4,273,361	-		PP_3744	0.35	-0.1
4,273,479	-		PP_3744	0.50	+1.1
4,273,606	+		PP_3744	0.66	+0.8
4,273,606	+		PP_3744	0.66	+0.2
4,273,884	+				+0.6
4,273,885	+				+0.7
4,273,885	-				+0.4
4,273,917	+				+1.6
4,273,917	+				-0.6
4,274,049	+				+0.5
4,274,050	-				-0.2
4,274,198	-		PP_3745	0.10	+0.3
4,274,376	+		PP_3745	0.22	+0.8
4,274,427	-		PP_3745	0.26	+0.7
4,274,466	+		PP_3745	0.28	-1.1
4,274,466	+		PP_3745	0.28	+0.1
4,274,466	+		PP_3745	0.28	+1.0
4,274,466	+		PP_3745	0.28	+0.5
4,274,467	-		PP_3745	0.28	-0.3
4,274,467	-		PP_3745	0.28	-0.7
4,274,467	-		PP_3745	0.28	+0.6
4,274,467	-		PP_3745	0.28	+0.4
4,274,467	-		PP_3745	0.28	+0.0
4,274,467	-		PP_3745	0.28	-0.6
4,274,470	+		PP_3745	0.28	+0.8
4,274,470	+		PP_3745	0.28	-0.7
4,274,470	+		PP_3745	0.28	-1.6
4,274,471	-		PP_3745	0.28	-0.2
4,274,471	-		PP_3745	0.28	+0.4
4,274,471	-		PP_3745	0.28	+0.1
4,274,473	+		PP_3745	0.29	+0.5
4,274,473	+		PP_3745	0.29	+1.2
4,274,473	+		PP_3745	0.29	+0.5
4,274,473	+		PP_3745	0.29	-0.3
4,274,474	-		PP_3745	0.29	-0.9
4,274,474	-		PP_3745	0.29	+0.1
4,274,557	+		PP_3745	0.34	-0.2
4,274,558	-		PP_3745	0.34	+0.8
4,274,558	-		PP_3745	0.34	-0.7
4,274,598	+		PP_3745	0.37	-0.7
4,274,599	-		PP_3745	0.37	+0.3
4,274,599	-		PP_3745	0.37	+0.2
4,274,753	+		PP_3745	0.47	-0.8