Strain Fitness in Pseudomonas putida KT2440 around PP_3235

Experiment: 3-methyl-3-butenol (commercial) 0.25 vol%

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-3-butenol (commercial) 0.25 vol%
3,671,014	+				-0.8
3,671,015	-		PP_5591	0.84	+0.4
3,671,015	-		PP_5591	0.84	-0.6
3,671,173	+				+0.8
3,671,174	-				-0.1
3,671,303	-		PP_3234	0.15	+0.3
3,671,715	+		PP_3234	0.75	-0.3
3,671,715	+		PP_3234	0.75	-2.1
3,671,716	-		PP_3234	0.75	-0.1
3,671,716	-		PP_3234	0.75	-0.6
3,671,885	+				+0.5
3,671,886	-				-0.1
3,671,941	+				+1.8
3,671,942	-				+1.5
3,671,965	+				-0.3
3,671,966	-				-0.0
3,671,966	-				-2.1
3,671,966	-				-0.7
3,672,000	+		PP_3235	0.13	+1.6
3,672,001	-		PP_3235	0.13	+0.4
3,672,010	+		PP_3235	0.15	+0.1
3,672,011	-		PP_3235	0.16	+1.9
3,672,011	-		PP_3235	0.16	+0.2
3,672,038	-		PP_3235	0.22	+0.5
3,672,038	-		PP_3235	0.22	+1.2
3,672,179	+		PP_3235	0.58	-0.0
3,672,180	-		PP_3235	0.59	+0.3
3,672,240	+		PP_3235	0.74	+0.6
3,672,241	-		PP_3235	0.74	-0.0
3,672,256	-		PP_3235	0.78	-0.6
3,672,434	+				-0.3
3,672,506	-				+0.3
3,672,550	+				-0.2
3,672,550	+				+1.1
3,672,551	-				-1.2
3,672,551	-				+1.4
3,672,551	-				-0.4
3,672,623	-		PP_3236	0.33	+0.8
3,672,821	+				-0.0
3,672,948	+				-0.3
3,672,963	-				-0.1
3,673,099	-		PP_3237	0.16	+0.7