Experiment: 3-methyl-3-butenol (commercial) 0.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3114 and PP_3115 are separated by 14 nucleotides PP_3115 and PP_3116 are separated by 405 nucleotides PP_3116 and PP_3117 overlap by 1 nucleotides PP_3117 and PP_3118 are separated by 6 nucleotides
PP_3114: PP_3114 - transposase, at 3,523,288 to 3,524,820
_3114
PP_3115: PP_3115 - transposase, at 3,524,835 to 3,525,149
_3115
PP_3116: PP_3116 - transcriptional repressor, at 3,525,555 to 3,526,163
_3116
PP_3117: PP_3117 - putative protein involved in error-prone processing of DNA lesions, at 3,526,163 to 3,526,783
_3117
PP_3118: PP_3118 - DNA linked enzyme involved in DNA repair, at 3,526,790 to 3,528,208
_3118
Position (kb)
3525
3526
3527 Strain fitness (log2 ratio)
-1
0
1 at 3525.192 kb on + strand at 3525.192 kb on + strand at 3525.192 kb on + strand at 3525.192 kb on + strand at 3525.422 kb on - strand at 3525.493 kb on - strand at 3525.493 kb on - strand at 3525.493 kb on - strand at 3525.566 kb on + strand at 3525.566 kb on + strand at 3525.567 kb on - strand at 3525.718 kb on - strand, within PP_3116 at 3525.751 kb on + strand, within PP_3116 at 3525.751 kb on + strand, within PP_3116 at 3525.793 kb on + strand, within PP_3116 at 3526.086 kb on + strand, within PP_3116 at 3526.086 kb on + strand, within PP_3116 at 3526.479 kb on + strand, within PP_3117 at 3526.479 kb on + strand, within PP_3117 at 3526.605 kb on + strand, within PP_3117 at 3526.605 kb on + strand, within PP_3117 at 3526.761 kb on + strand at 3526.762 kb on - strand at 3527.055 kb on - strand, within PP_3118 at 3527.085 kb on - strand, within PP_3118 at 3527.085 kb on - strand, within PP_3118
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-3-butenol (commercial) 0.25 vol% remove 3,525,192 + -0.4 3,525,192 + +0.3 3,525,192 + -0.5 3,525,192 + +0.1 3,525,422 - +0.6 3,525,493 - -0.6 3,525,493 - +1.3 3,525,493 - +0.3 3,525,566 + +0.4 3,525,566 + -0.2 3,525,567 - +1.7 3,525,718 - PP_3116 0.27 +0.7 3,525,751 + PP_3116 0.32 +0.6 3,525,751 + PP_3116 0.32 +1.2 3,525,793 + PP_3116 0.39 +0.3 3,526,086 + PP_3116 0.87 +0.4 3,526,086 + PP_3116 0.87 +0.3 3,526,479 + PP_3117 0.51 -0.2 3,526,479 + PP_3117 0.51 +0.0 3,526,605 + PP_3117 0.71 -0.4 3,526,605 + PP_3117 0.71 -0.9 3,526,761 + -1.4 3,526,762 - +0.9 3,527,055 - PP_3118 0.19 +0.6 3,527,085 - PP_3118 0.21 +1.1 3,527,085 - PP_3118 0.21 -0.2
Or see this region's nucleotide sequence