Experiment: 3-methyl-3-butenol (commercial) 0.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0665 and PP_0666 are separated by 16 nucleotides PP_0666 and PP_0667 are separated by 170 nucleotides PP_0667 and PP_0668 overlap by 4 nucleotides
PP_0665: PP_0665 - putative glyceraldehyde-3-phosphate dehydrogenase, at 773,394 to 774,812
_0665
PP_0666: PP_0666 - conserved protein of unknown function, at 774,829 to 775,191
_0666
PP_0667: PP_0667 - RNA polymerase sigma-70 factor, ECF subfamily, at 775,362 to 775,898
_0667
PP_0668: PP_0668 - putative Transmembrane sensor, at 775,895 to 776,860
_0668
Position (kb) 774
775
776 Strain fitness (log2 ratio) -1
0
1 at 773.832 kb on - strand, within PP_0665 at 773.956 kb on - strand, within PP_0665 at 773.996 kb on - strand, within PP_0665 at 774.253 kb on + strand, within PP_0665 at 774.254 kb on - strand, within PP_0665 at 774.268 kb on - strand, within PP_0665 at 774.268 kb on - strand, within PP_0665 at 774.456 kb on + strand, within PP_0665 at 774.589 kb on - strand, within PP_0665 at 774.589 kb on - strand, within PP_0665 at 774.650 kb on + strand, within PP_0665 at 774.667 kb on + strand, within PP_0665 at 774.667 kb on + strand, within PP_0665 at 774.667 kb on + strand, within PP_0665 at 774.667 kb on + strand, within PP_0665 at 774.668 kb on - strand, within PP_0665 at 774.668 kb on - strand, within PP_0665 at 774.726 kb on + strand at 774.726 kb on + strand at 774.726 kb on + strand at 774.727 kb on - strand at 774.727 kb on - strand at 774.727 kb on - strand at 774.763 kb on - strand at 774.822 kb on + strand at 774.823 kb on - strand at 774.823 kb on - strand at 774.823 kb on - strand at 775.066 kb on + strand, within PP_0666 at 775.066 kb on + strand, within PP_0666 at 775.066 kb on + strand, within PP_0666 at 775.067 kb on - strand, within PP_0666 at 775.067 kb on - strand, within PP_0666 at 775.136 kb on - strand, within PP_0666 at 775.225 kb on + strand at 775.413 kb on + strand at 775.414 kb on - strand at 775.498 kb on + strand, within PP_0667 at 775.557 kb on + strand, within PP_0667 at 775.558 kb on - strand, within PP_0667 at 775.568 kb on + strand, within PP_0667 at 775.568 kb on + strand, within PP_0667 at 775.568 kb on + strand, within PP_0667 at 775.568 kb on + strand, within PP_0667 at 775.568 kb on + strand, within PP_0667 at 775.568 kb on + strand, within PP_0667 at 775.569 kb on - strand, within PP_0667 at 775.727 kb on + strand, within PP_0667 at 775.727 kb on + strand, within PP_0667 at 775.728 kb on - strand, within PP_0667 at 775.833 kb on + strand, within PP_0667 at 775.834 kb on - strand, within PP_0667 at 775.847 kb on + strand at 775.847 kb on + strand at 775.847 kb on + strand at 775.848 kb on - strand at 775.848 kb on - strand at 775.848 kb on - strand at 775.848 kb on - strand at 775.848 kb on - strand at 775.848 kb on - strand at 775.851 kb on + strand at 775.851 kb on + strand at 775.851 kb on + strand at 775.851 kb on + strand at 775.852 kb on - strand at 775.852 kb on - strand at 775.852 kb on - strand at 776.043 kb on - strand, within PP_0668 at 776.047 kb on - strand, within PP_0668 at 776.047 kb on - strand, within PP_0668 at 776.047 kb on - strand, within PP_0668
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-3-butenol (commercial) 0.25 vol% remove 773,832 - PP_0665 0.31 +0.4 773,956 - PP_0665 0.40 -1.0 773,996 - PP_0665 0.42 -0.9 774,253 + PP_0665 0.61 +0.7 774,254 - PP_0665 0.61 -0.6 774,268 - PP_0665 0.62 -0.7 774,268 - PP_0665 0.62 +0.1 774,456 + PP_0665 0.75 +0.4 774,589 - PP_0665 0.84 +1.1 774,589 - PP_0665 0.84 +0.2 774,650 + PP_0665 0.89 +0.1 774,667 + PP_0665 0.90 -0.1 774,667 + PP_0665 0.90 +0.0 774,667 + PP_0665 0.90 +0.0 774,667 + PP_0665 0.90 +1.0 774,668 - PP_0665 0.90 -0.1 774,668 - PP_0665 0.90 +0.3 774,726 + +0.5 774,726 + +0.8 774,726 + +0.7 774,727 - -0.9 774,727 - +0.6 774,727 - +1.3 774,763 - -0.0 774,822 + -1.3 774,823 - -0.9 774,823 - +0.3 774,823 - -0.4 775,066 + PP_0666 0.65 +0.0 775,066 + PP_0666 0.65 -1.1 775,066 + PP_0666 0.65 -0.5 775,067 - PP_0666 0.66 -0.6 775,067 - PP_0666 0.66 -0.9 775,136 - PP_0666 0.85 -0.1 775,225 + -0.0 775,413 + -0.0 775,414 - -0.8 775,498 + PP_0667 0.25 -0.4 775,557 + PP_0667 0.36 +0.6 775,558 - PP_0667 0.36 +0.8 775,568 + PP_0667 0.38 -0.1 775,568 + PP_0667 0.38 -0.1 775,568 + PP_0667 0.38 +0.4 775,568 + PP_0667 0.38 +0.2 775,568 + PP_0667 0.38 +0.7 775,568 + PP_0667 0.38 -0.9 775,569 - PP_0667 0.39 +0.9 775,727 + PP_0667 0.68 +0.1 775,727 + PP_0667 0.68 -0.0 775,728 - PP_0667 0.68 +0.4 775,833 + PP_0667 0.88 -0.5 775,834 - PP_0667 0.88 +0.1 775,847 + -0.1 775,847 + +1.1 775,847 + +0.8 775,848 - +0.2 775,848 - +0.6 775,848 - -1.0 775,848 - +1.5 775,848 - +0.7 775,848 - -0.6 775,851 + +0.4 775,851 + -0.3 775,851 + -0.6 775,851 + -0.1 775,852 - +0.3 775,852 - -0.2 775,852 - +0.4 776,043 - PP_0668 0.15 -0.3 776,047 - PP_0668 0.16 +0.1 776,047 - PP_0668 0.16 +0.1 776,047 - PP_0668 0.16 +0.5
Or see this region's nucleotide sequence