Strain Fitness in Escherichia coli BW25113 around b2591

Experiment: 3-methyl-1-butanol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrrlG and gltW are separated by 184 nucleotidesgltW and rrsG are separated by 171 nucleotidesrrsG and clpB are separated by 442 nucleotides b2589: rrlG - 23S ribosomal RNA (NCBI), at 2,724,303 to 2,727,206 rrlG b2590: gltW - tRNA-Glu (NCBI), at 2,727,391 to 2,727,466 gltW b2591: rrsG - 16S ribosomal RNA (NCBI), at 2,727,638 to 2,729,179 rrsG b2592: clpB - protein disaggregation chaperone (NCBI), at 2,729,622 to 2,732,195 clpB Position (kb) 2727 2728 2729 2730Strain fitness (log2 ratio) -2 -1 0 1at 2729.412 kb on - strandat 2729.705 kb on - strandat 2729.753 kb on + strandat 2729.753 kb on + strandat 2729.761 kb on - strandat 2729.900 kb on + strand, within clpBat 2730.084 kb on + strand, within clpBat 2730.092 kb on - strand, within clpBat 2730.173 kb on + strand, within clpB

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol%
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2,729,412 - -2.1
2,729,705 - -0.5
2,729,753 + -1.5
2,729,753 + -2.7
2,729,761 - -0.3
2,729,900 + clpB b2592 0.11 +1.1
2,730,084 + clpB b2592 0.18 -0.8
2,730,092 - clpB b2592 0.18 +0.6
2,730,173 + clpB b2592 0.21 -1.4

Or see this region's nucleotide sequence