Strain Fitness in Escherichia coli BW25113 around b4211

Experiment: 3-methyl-1-butanol 1.25 vol%

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-1-butanol 1.25 vol%
4,430,189	-				+0.7
4,430,189	-				-2.4
4,430,189	-				-0.6
4,430,189	-				-0.3
4,430,189	-				+0.5
4,430,196	+				-0.7
4,430,199	-				+1.6
4,430,209	-				+0.8
4,430,320	+	ytfF	b4210	0.21	+1.5
4,430,335	-	ytfF	b4210	0.23	+2.0
4,430,335	-	ytfF	b4210	0.23	+0.8
4,430,408	-	ytfF	b4210	0.30	-1.8
4,430,456	-	ytfF	b4210	0.35	-3.5
4,430,557	+	ytfF	b4210	0.46	+0.4
4,430,565	-	ytfF	b4210	0.47	+0.7
4,430,565	-	ytfF	b4210	0.47	+0.9
4,430,591	+	ytfF	b4210	0.49	-1.0
4,430,739	+	ytfF	b4210	0.65	+0.5
4,430,739	+	ytfF	b4210	0.65	-0.1
4,430,744	+	ytfF	b4210	0.65	-2.0
4,430,744	+	ytfF	b4210	0.65	-1.3
4,430,809	+	ytfF	b4210	0.72	+1.3
4,430,809	+	ytfF	b4210	0.72	-0.2
4,430,849	-	ytfF	b4210	0.76	-0.5
4,430,849	-	ytfF	b4210	0.76	-0.4
4,430,853	+	ytfF	b4210	0.77	-1.2
4,430,853	+	ytfF	b4210	0.77	+1.4
4,430,861	-	ytfF	b4210	0.77	+1.8
4,430,861	-	ytfF	b4210	0.77	-0.7
4,430,878	-	ytfF	b4210	0.79	-2.8
4,430,887	-	ytfF	b4210	0.80	-0.2
4,430,942	-	ytfF	b4210	0.86	-0.0
4,430,955	-	ytfF	b4210	0.87	+1.5
4,430,955	-	ytfF	b4210	0.87	+0.7
4,430,956	-	ytfF	b4210	0.87	+0.9
4,430,988	-				-2.4
4,431,049	+				-0.8
4,431,051	-				-2.0
4,431,055	-				-1.9
4,431,065	-				+0.2
4,431,115	+				-1.4
4,431,115	+				+0.4
4,431,169	-				+2.5
4,431,219	-				+1.9
4,431,290	-	ytfG	b4211	0.12	+0.2
4,431,332	+	ytfG	b4211	0.17	+0.6
4,431,350	-	ytfG	b4211	0.19	+1.4
4,431,350	-	ytfG	b4211	0.19	+3.8
4,431,448	-	ytfG	b4211	0.30	-0.6
4,431,458	+	ytfG	b4211	0.31	+0.3
4,431,498	+	ytfG	b4211	0.36	-0.1
4,431,498	+	ytfG	b4211	0.36	+1.6
4,431,657	-	ytfG	b4211	0.55	+1.2
4,431,749	-	ytfG	b4211	0.65	-0.5
4,431,881	-	ytfG	b4211	0.81	+0.5
4,431,954	-	ytfG	b4211	0.89	+1.0
4,431,958	+	ytfG	b4211	0.90	+1.1
4,432,182	+	ytfH	b4212	0.12	+0.1
4,432,182	+	ytfH	b4212	0.12	-0.2
4,432,247	-	ytfH	b4212	0.29	-0.7
4,432,330	+	ytfH	b4212	0.51	+1.4
4,432,330	+	ytfH	b4212	0.51	-2.0
4,432,358	+	ytfH	b4212	0.58	+1.3
4,432,377	+	ytfH	b4212	0.63	-1.4
4,432,377	+	ytfH	b4212	0.63	+0.8
4,432,402	+	ytfH	b4212	0.70	+0.7
4,432,407	+	ytfH	b4212	0.71	-0.7
4,432,441	-	ytfH	b4212	0.80	-1.0
4,432,482	+				-0.0
4,432,498	+				-1.3
4,432,531	+				+0.1
4,432,572	-				+0.6
4,432,648	-				-0.7
4,432,659	-				+1.9
4,432,659	-				-1.3
4,432,717	+				+1.3
4,432,719	-				-1.1
4,432,719	-				+0.8
4,432,741	+				+0.8
4,432,847	+	cpdB	b4213	0.10	+0.7
4,432,847	+	cpdB	b4213	0.10	-1.2
4,432,855	+	cpdB	b4213	0.11	-0.0
4,432,855	+	cpdB	b4213	0.11	+0.3
4,432,874	+	cpdB	b4213	0.12	-0.3
4,432,881	-	cpdB	b4213	0.12	+0.6
4,432,881	-	cpdB	b4213	0.12	-1.4
4,432,902	+	cpdB	b4213	0.13	+1.9
4,432,913	+	cpdB	b4213	0.14	-1.4
4,433,022	+	cpdB	b4213	0.19	-0.6