Strain Fitness in Escherichia coli BW25113 around b4209

Experiment: 3-methyl-1-butanol 1.25 vol%

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-1-butanol 1.25 vol%
4,428,375	-	cycA	b4208	0.35	+1.7
4,428,398	+	cycA	b4208	0.36	+1.4
4,428,422	+	cycA	b4208	0.38	+0.9
4,428,427	+	cycA	b4208	0.38	-1.4
4,428,454	-	cycA	b4208	0.40	+1.8
4,428,589	-	cycA	b4208	0.50	+2.5
4,428,690	-	cycA	b4208	0.57	+1.5
4,428,692	-	cycA	b4208	0.57	-0.1
4,428,742	-	cycA	b4208	0.61	-1.2
4,428,752	+	cycA	b4208	0.61	-1.1
4,428,752	+	cycA	b4208	0.61	-1.1
4,428,765	-	cycA	b4208	0.62	+0.1
4,428,765	-	cycA	b4208	0.62	+1.6
4,428,793	+	cycA	b4208	0.64	-0.7
4,428,793	+	cycA	b4208	0.64	+3.0
4,428,793	+	cycA	b4208	0.64	+0.7
4,428,793	+	cycA	b4208	0.64	-1.8
4,428,793	+	cycA	b4208	0.64	+2.3
4,428,793	+	cycA	b4208	0.64	+0.8
4,428,850	-	cycA	b4208	0.68	+3.3
4,428,880	-	cycA	b4208	0.70	+2.8
4,428,980	+	cycA	b4208	0.77	+0.7
4,428,980	+	cycA	b4208	0.77	-0.5
4,428,985	+	cycA	b4208	0.78	-0.5
4,429,009	+	cycA	b4208	0.79	-1.1
4,429,087	-	cycA	b4208	0.85	-0.4
4,429,088	+	cycA	b4208	0.85	+1.2
4,429,111	+	cycA	b4208	0.87	+0.8
4,429,156	-	cycA	b4208	0.90	+1.0
4,429,159	-				+0.3
4,429,176	-				+1.5
4,429,185	-				-1.6
4,429,195	+				-0.7
4,429,221	-				-1.1
4,429,221	-				+3.0
4,429,254	-				+2.4
4,429,280	+				+1.9
4,429,315	+				+0.6
4,429,361	+				+1.0
4,429,373	+				-1.3
4,429,453	+	ytfE	b4209	0.16	+1.0
4,429,453	+	ytfE	b4209	0.16	-2.5
4,429,463	-	ytfE	b4209	0.18	-1.1
4,429,463	-	ytfE	b4209	0.18	-0.0
4,429,466	-	ytfE	b4209	0.18	-0.1
4,429,501	-	ytfE	b4209	0.24	+1.0
4,429,559	+	ytfE	b4209	0.32	-2.2
4,429,559	+	ytfE	b4209	0.32	+1.6
4,429,631	-	ytfE	b4209	0.43	-0.1
4,429,631	-	ytfE	b4209	0.43	-0.4
4,429,798	+	ytfE	b4209	0.68	-0.1
4,429,821	+	ytfE	b4209	0.72	+0.1
4,429,857	-	ytfE	b4209	0.77	-1.1
4,429,907	-	ytfE	b4209	0.85	+0.1
4,429,935	-	ytfE	b4209	0.89	+3.2
4,429,935	-	ytfE	b4209	0.89	-1.1
4,430,003	+				-0.6
4,430,189	-				+0.7
4,430,189	-				-2.4
4,430,189	-				-0.6
4,430,189	-				-0.3
4,430,189	-				+0.5
4,430,196	+				-0.7
4,430,199	-				+1.6
4,430,209	-				+0.8
4,430,320	+	ytfF	b4210	0.21	+1.5
4,430,335	-	ytfF	b4210	0.23	+2.0
4,430,335	-	ytfF	b4210	0.23	+0.8
4,430,408	-	ytfF	b4210	0.30	-1.8
4,430,456	-	ytfF	b4210	0.35	-3.5
4,430,557	+	ytfF	b4210	0.46	+0.4
4,430,565	-	ytfF	b4210	0.47	+0.7
4,430,565	-	ytfF	b4210	0.47	+0.9
4,430,591	+	ytfF	b4210	0.49	-1.0
4,430,739	+	ytfF	b4210	0.65	+0.5
4,430,739	+	ytfF	b4210	0.65	-0.1
4,430,744	+	ytfF	b4210	0.65	-2.0
4,430,744	+	ytfF	b4210	0.65	-1.3
4,430,809	+	ytfF	b4210	0.72	+1.3
4,430,809	+	ytfF	b4210	0.72	-0.2
4,430,849	-	ytfF	b4210	0.76	-0.5
4,430,849	-	ytfF	b4210	0.76	-0.4
4,430,853	+	ytfF	b4210	0.77	-1.2
4,430,853	+	ytfF	b4210	0.77	+1.4
4,430,861	-	ytfF	b4210	0.77	+1.8
4,430,861	-	ytfF	b4210	0.77	-0.7
4,430,878	-	ytfF	b4210	0.79	-2.8
4,430,887	-	ytfF	b4210	0.80	-0.2
4,430,942	-	ytfF	b4210	0.86	-0.0
4,430,955	-	ytfF	b4210	0.87	+1.5
4,430,955	-	ytfF	b4210	0.87	+0.7
4,430,956	-	ytfF	b4210	0.87	+0.9
4,430,988	-				-2.4