Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygcQ and ygcR overlap by 4 nucleotides ygcR and ygcS overlap by 23 nucleotides
b2769: ygcQ - putative flavoprotein (VIMSS), at 2,892,941 to 2,893,801
ygcQ
b2770: ygcR - putative transport protein (VIMSS), at 2,893,798 to 2,894,577
ygcR
b2771: ygcS - putative transport protein (VIMSS), at 2,894,555 to 2,895,892
ygcS
Position (kb)
2893
2894
2895 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2892.854 kb on - strand at 2892.888 kb on - strand at 2892.888 kb on - strand at 2892.914 kb on - strand at 2892.914 kb on - strand at 2892.914 kb on - strand at 2892.939 kb on + strand at 2892.973 kb on - strand at 2892.993 kb on - strand at 2893.003 kb on - strand at 2893.184 kb on + strand, within ygcQ at 2893.195 kb on - strand, within ygcQ at 2893.279 kb on - strand, within ygcQ at 2893.284 kb on - strand, within ygcQ at 2893.368 kb on - strand, within ygcQ at 2893.552 kb on + strand, within ygcQ at 2893.566 kb on - strand, within ygcQ at 2893.636 kb on - strand, within ygcQ at 2893.657 kb on - strand, within ygcQ at 2893.717 kb on + strand at 2893.825 kb on + strand at 2893.825 kb on + strand at 2893.883 kb on - strand, within ygcR at 2893.891 kb on - strand, within ygcR at 2893.896 kb on - strand, within ygcR at 2893.959 kb on + strand, within ygcR at 2894.040 kb on + strand, within ygcR at 2894.040 kb on + strand, within ygcR at 2894.141 kb on - strand, within ygcR at 2894.260 kb on - strand, within ygcR at 2894.334 kb on - strand, within ygcR at 2894.334 kb on - strand, within ygcR at 2894.362 kb on + strand, within ygcR at 2894.372 kb on + strand, within ygcR at 2894.372 kb on + strand, within ygcR at 2894.373 kb on - strand, within ygcR at 2894.373 kb on - strand, within ygcR at 2894.500 kb on - strand at 2894.638 kb on + strand at 2894.756 kb on + strand, within ygcS at 2894.873 kb on + strand, within ygcS at 2894.873 kb on + strand, within ygcS at 2894.892 kb on + strand, within ygcS at 2894.999 kb on - strand, within ygcS at 2895.130 kb on - strand, within ygcS at 2895.187 kb on + strand, within ygcS at 2895.187 kb on + strand, within ygcS at 2895.442 kb on - strand, within ygcS at 2895.535 kb on - strand, within ygcS at 2895.559 kb on + strand, within ygcS
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 2,892,854 - +0.3 2,892,888 - +0.9 2,892,888 - -0.6 2,892,914 - +1.9 2,892,914 - -0.5 2,892,914 - -0.1 2,892,939 + -0.8 2,892,973 - +1.4 2,892,993 - +2.2 2,893,003 - +0.8 2,893,184 + ygcQ b2769 0.28 -1.6 2,893,195 - ygcQ b2769 0.30 -0.2 2,893,279 - ygcQ b2769 0.39 -1.1 2,893,284 - ygcQ b2769 0.40 -0.7 2,893,368 - ygcQ b2769 0.50 +0.7 2,893,552 + ygcQ b2769 0.71 -1.4 2,893,566 - ygcQ b2769 0.73 -1.7 2,893,636 - ygcQ b2769 0.81 -0.1 2,893,657 - ygcQ b2769 0.83 +0.4 2,893,717 + +0.7 2,893,825 + -0.4 2,893,825 + +0.3 2,893,883 - ygcR b2770 0.11 +0.4 2,893,891 - ygcR b2770 0.12 +0.3 2,893,896 - ygcR b2770 0.13 -0.2 2,893,959 + ygcR b2770 0.21 +1.1 2,894,040 + ygcR b2770 0.31 +1.0 2,894,040 + ygcR b2770 0.31 +0.1 2,894,141 - ygcR b2770 0.44 +1.5 2,894,260 - ygcR b2770 0.59 -0.7 2,894,334 - ygcR b2770 0.69 +1.7 2,894,334 - ygcR b2770 0.69 -0.4 2,894,362 + ygcR b2770 0.72 +3.1 2,894,372 + ygcR b2770 0.74 -1.1 2,894,372 + ygcR b2770 0.74 +0.6 2,894,373 - ygcR b2770 0.74 -0.6 2,894,373 - ygcR b2770 0.74 -1.3 2,894,500 - -2.0 2,894,638 + -1.6 2,894,756 + ygcS b2771 0.15 -0.3 2,894,873 + ygcS b2771 0.24 -0.3 2,894,873 + ygcS b2771 0.24 +0.3 2,894,892 + ygcS b2771 0.25 +0.3 2,894,999 - ygcS b2771 0.33 +1.0 2,895,130 - ygcS b2771 0.43 -0.7 2,895,187 + ygcS b2771 0.47 -1.4 2,895,187 + ygcS b2771 0.47 -2.0 2,895,442 - ygcS b2771 0.66 -0.8 2,895,535 - ygcS b2771 0.73 +1.4 2,895,559 + ygcS b2771 0.75 -0.0
Or see this region's nucleotide sequence