Strain Fitness in Escherichia coli BW25113 around b2484

Experiment: 3-methyl-1-butanol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthyfB and hyfC are separated by 10 nucleotideshyfC and hyfD are separated by 16 nucleotideshyfD and hyfE are separated by 11 nucleotideshyfE and hyfF are separated by 4 nucleotides b2482: hyfB - NADH dehydrogenase subunit N (NCBI), at 2,599,840 to 2,601,858 hyfB b2483: hyfC - hydrogenase 4 membrane subunit (VIMSS), at 2,601,869 to 2,602,816 hyfC b2484: hyfD - hydrogenase 4 membrane subunit (NCBI), at 2,602,833 to 2,604,272 hyfD b2485: hyfE - hydrogenase 4, membrane subunit (NCBI), at 2,604,284 to 2,604,934 hyfE b2486: hyfF - NADH dehydrogenase subunit N (NCBI), at 2,604,939 to 2,606,519 hyfF Position (kb) 2602 2603 2604 2605Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2601.925 kb on + strandat 2601.925 kb on + strandat 2601.988 kb on - strand, within hyfCat 2602.127 kb on + strand, within hyfCat 2602.127 kb on + strand, within hyfCat 2602.150 kb on - strand, within hyfCat 2602.199 kb on - strand, within hyfCat 2602.199 kb on - strand, within hyfCat 2602.325 kb on + strand, within hyfCat 2602.350 kb on + strand, within hyfCat 2602.379 kb on + strand, within hyfCat 2602.382 kb on - strand, within hyfCat 2602.432 kb on - strand, within hyfCat 2602.620 kb on - strand, within hyfCat 2602.620 kb on - strand, within hyfCat 2602.813 kb on + strandat 2602.813 kb on + strandat 2603.063 kb on - strand, within hyfDat 2603.095 kb on - strand, within hyfDat 2603.151 kb on + strand, within hyfDat 2603.151 kb on + strand, within hyfDat 2603.293 kb on + strand, within hyfDat 2603.301 kb on - strand, within hyfDat 2603.328 kb on - strand, within hyfDat 2603.369 kb on + strand, within hyfDat 2603.430 kb on + strand, within hyfDat 2603.464 kb on - strand, within hyfDat 2603.464 kb on - strand, within hyfDat 2603.477 kb on - strand, within hyfDat 2603.547 kb on - strand, within hyfDat 2603.570 kb on - strand, within hyfDat 2603.574 kb on + strand, within hyfDat 2603.574 kb on + strand, within hyfDat 2603.613 kb on + strand, within hyfDat 2603.716 kb on + strand, within hyfDat 2603.726 kb on + strand, within hyfDat 2603.726 kb on + strand, within hyfDat 2603.783 kb on + strand, within hyfDat 2603.882 kb on + strand, within hyfDat 2603.882 kb on + strand, within hyfDat 2603.933 kb on - strand, within hyfDat 2603.933 kb on - strand, within hyfDat 2604.064 kb on - strand, within hyfDat 2604.064 kb on - strand, within hyfDat 2604.216 kb on + strandat 2604.216 kb on + strandat 2604.284 kb on - strandat 2604.284 kb on - strandat 2604.340 kb on + strandat 2604.488 kb on + strand, within hyfEat 2604.488 kb on + strand, within hyfEat 2604.513 kb on - strand, within hyfEat 2604.598 kb on - strand, within hyfEat 2604.626 kb on + strand, within hyfEat 2604.700 kb on + strand, within hyfEat 2604.708 kb on - strand, within hyfEat 2604.708 kb on - strand, within hyfEat 2604.828 kb on + strand, within hyfEat 2604.862 kb on - strand, within hyfEat 2604.882 kb on + strandat 2604.882 kb on + strandat 2605.055 kb on - strandat 2605.065 kb on - strandat 2605.065 kb on - strandat 2605.071 kb on - strandat 2605.140 kb on - strand, within hyfFat 2605.210 kb on + strand, within hyfFat 2605.256 kb on + strand, within hyfF

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol%
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2,601,925 + +0.6
2,601,925 + +1.2
2,601,988 - hyfC b2483 0.13 -1.2
2,602,127 + hyfC b2483 0.27 -1.7
2,602,127 + hyfC b2483 0.27 -1.3
2,602,150 - hyfC b2483 0.30 -0.9
2,602,199 - hyfC b2483 0.35 -0.3
2,602,199 - hyfC b2483 0.35 +0.7
2,602,325 + hyfC b2483 0.48 +0.2
2,602,350 + hyfC b2483 0.51 -0.7
2,602,379 + hyfC b2483 0.54 -0.9
2,602,382 - hyfC b2483 0.54 -1.1
2,602,432 - hyfC b2483 0.59 -0.1
2,602,620 - hyfC b2483 0.79 -1.5
2,602,620 - hyfC b2483 0.79 +0.7
2,602,813 + +0.8
2,602,813 + -0.3
2,603,063 - hyfD b2484 0.16 -1.6
2,603,095 - hyfD b2484 0.18 -0.2
2,603,151 + hyfD b2484 0.22 +0.6
2,603,151 + hyfD b2484 0.22 +0.9
2,603,293 + hyfD b2484 0.32 +0.7
2,603,301 - hyfD b2484 0.33 -2.7
2,603,328 - hyfD b2484 0.34 -1.3
2,603,369 + hyfD b2484 0.37 -0.2
2,603,430 + hyfD b2484 0.41 -1.9
2,603,464 - hyfD b2484 0.44 -1.0
2,603,464 - hyfD b2484 0.44 +0.3
2,603,477 - hyfD b2484 0.45 +1.6
2,603,547 - hyfD b2484 0.50 -1.1
2,603,570 - hyfD b2484 0.51 -1.0
2,603,574 + hyfD b2484 0.51 -0.1
2,603,574 + hyfD b2484 0.51 -0.4
2,603,613 + hyfD b2484 0.54 -1.2
2,603,716 + hyfD b2484 0.61 -0.7
2,603,726 + hyfD b2484 0.62 -0.7
2,603,726 + hyfD b2484 0.62 -0.3
2,603,783 + hyfD b2484 0.66 -0.8
2,603,882 + hyfD b2484 0.73 -1.6
2,603,882 + hyfD b2484 0.73 +1.9
2,603,933 - hyfD b2484 0.76 +1.3
2,603,933 - hyfD b2484 0.76 -0.4
2,604,064 - hyfD b2484 0.85 +0.2
2,604,064 - hyfD b2484 0.85 +1.5
2,604,216 + +3.1
2,604,216 + -1.3
2,604,284 - -2.2
2,604,284 - -0.3
2,604,340 + -0.6
2,604,488 + hyfE b2485 0.31 -2.2
2,604,488 + hyfE b2485 0.31 -2.3
2,604,513 - hyfE b2485 0.35 +1.9
2,604,598 - hyfE b2485 0.48 +1.7
2,604,626 + hyfE b2485 0.53 -0.4
2,604,700 + hyfE b2485 0.64 +0.6
2,604,708 - hyfE b2485 0.65 -0.6
2,604,708 - hyfE b2485 0.65 +0.2
2,604,828 + hyfE b2485 0.84 +0.5
2,604,862 - hyfE b2485 0.89 +0.7
2,604,882 + -0.6
2,604,882 + -0.5
2,605,055 - -0.2
2,605,065 - -1.3
2,605,065 - +0.7
2,605,071 - +0.1
2,605,140 - hyfF b2486 0.13 +0.3
2,605,210 + hyfF b2486 0.17 +0.7
2,605,256 + hyfF b2486 0.20 +0.4

Or see this region's nucleotide sequence