Strain Fitness in Escherichia coli BW25113 around b2481

Experiment: 3-methyl-1-butanol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgcvR and bcp overlap by 1 nucleotidesbcp and hyfA are separated by 252 nucleotideshyfA and hyfB overlap by 1 nucleotides b2479: gcvR - transcriptional regulation of gcv operon (VIMSS), at 2,597,928 to 2,598,500 gcvR b2480: bcp - thioredoxin-dependent thiol peroxidase (NCBI), at 2,598,500 to 2,598,970 bcp b2481: hyfA - hydrogenase 4 Fe-S subunit (VIMSS), at 2,599,223 to 2,599,840 hyfA b2482: hyfB - NADH dehydrogenase subunit N (NCBI), at 2,599,840 to 2,601,858 hyfB Position (kb) 2599 2600Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2598.282 kb on + strand, within gcvRat 2598.285 kb on + strand, within gcvRat 2598.303 kb on - strand, within gcvRat 2598.303 kb on - strand, within gcvRat 2598.346 kb on - strand, within gcvRat 2598.400 kb on - strand, within gcvRat 2598.400 kb on - strand, within gcvRat 2598.418 kb on - strand, within gcvRat 2598.427 kb on - strand, within gcvRat 2598.513 kb on + strandat 2598.621 kb on + strand, within bcpat 2598.621 kb on + strand, within bcpat 2598.628 kb on - strand, within bcpat 2598.639 kb on - strand, within bcpat 2598.644 kb on - strand, within bcpat 2598.714 kb on - strand, within bcpat 2598.757 kb on + strand, within bcpat 2598.792 kb on - strand, within bcpat 2598.792 kb on - strand, within bcpat 2598.938 kb on + strandat 2598.967 kb on - strandat 2598.976 kb on - strandat 2599.005 kb on + strandat 2599.230 kb on + strandat 2599.238 kb on - strandat 2599.319 kb on - strand, within hyfAat 2599.319 kb on - strand, within hyfAat 2599.362 kb on + strand, within hyfAat 2599.370 kb on - strand, within hyfAat 2599.426 kb on + strand, within hyfAat 2599.488 kb on - strand, within hyfAat 2599.528 kb on - strand, within hyfAat 2599.913 kb on - strandat 2599.998 kb on + strandat 2600.132 kb on + strand, within hyfBat 2600.135 kb on - strand, within hyfBat 2600.340 kb on - strand, within hyfBat 2600.517 kb on - strand, within hyfBat 2600.527 kb on + strand, within hyfBat 2600.577 kb on - strand, within hyfBat 2600.621 kb on - strand, within hyfBat 2600.621 kb on - strand, within hyfBat 2600.759 kb on - strand, within hyfB

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol%
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2,598,282 + gcvR b2479 0.62 +1.8
2,598,285 + gcvR b2479 0.62 +2.4
2,598,303 - gcvR b2479 0.65 -0.4
2,598,303 - gcvR b2479 0.65 -1.1
2,598,346 - gcvR b2479 0.73 +0.5
2,598,400 - gcvR b2479 0.82 +0.8
2,598,400 - gcvR b2479 0.82 +0.3
2,598,418 - gcvR b2479 0.86 +1.6
2,598,427 - gcvR b2479 0.87 -0.3
2,598,513 + -0.5
2,598,621 + bcp b2480 0.26 +0.3
2,598,621 + bcp b2480 0.26 -2.6
2,598,628 - bcp b2480 0.27 -0.2
2,598,639 - bcp b2480 0.30 -0.2
2,598,644 - bcp b2480 0.31 +0.6
2,598,714 - bcp b2480 0.45 -3.1
2,598,757 + bcp b2480 0.55 +0.5
2,598,792 - bcp b2480 0.62 +0.1
2,598,792 - bcp b2480 0.62 +0.0
2,598,938 + -0.2
2,598,967 - -0.1
2,598,976 - +0.2
2,599,005 + -0.5
2,599,230 + +1.3
2,599,238 - +1.8
2,599,319 - hyfA b2481 0.16 +0.8
2,599,319 - hyfA b2481 0.16 -1.8
2,599,362 + hyfA b2481 0.22 +0.8
2,599,370 - hyfA b2481 0.24 -1.3
2,599,426 + hyfA b2481 0.33 -1.8
2,599,488 - hyfA b2481 0.43 +0.2
2,599,528 - hyfA b2481 0.49 -0.2
2,599,913 - -0.6
2,599,998 + -0.7
2,600,132 + hyfB b2482 0.14 -0.3
2,600,135 - hyfB b2482 0.15 +0.4
2,600,340 - hyfB b2482 0.25 -0.6
2,600,517 - hyfB b2482 0.34 -1.1
2,600,527 + hyfB b2482 0.34 -0.3
2,600,577 - hyfB b2482 0.37 -0.5
2,600,621 - hyfB b2482 0.39 +1.7
2,600,621 - hyfB b2482 0.39 -0.1
2,600,759 - hyfB b2482 0.46 +0.6

Or see this region's nucleotide sequence