Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lhr and ydhD are separated by 50 nucleotides ydhD and ydhO are separated by 333 nucleotides ydhO and sodB are separated by 127 nucleotides sodB and ydhP are separated by 161 nucleotides
b1653: lhr - predicted ATP-dependent helicase (NCBI), at 1,727,111 to 1,731,727
lhr
b1654: ydhD - hypothetical protein (NCBI), at 1,731,778 to 1,732,125
ydhD
b1655: ydhO - predicted lipoprotein (NCBI), at 1,732,459 to 1,733,274
ydhO
b1656: sodB - superoxide dismutase, Fe (NCBI), at 1,733,402 to 1,733,983
sodB
b1657: ydhP - predicted transporter (NCBI), at 1,734,145 to 1,735,314
ydhP
Position (kb)
1732
1733
1734 Strain fitness (log2 ratio)
-1
0
1 at 1731.640 kb on - strand at 1731.728 kb on - strand at 1732.385 kb on - strand at 1733.172 kb on + strand, within ydhO at 1733.172 kb on + strand, within ydhO at 1733.225 kb on + strand at 1733.425 kb on + strand at 1733.522 kb on + strand, within sodB at 1733.612 kb on + strand, within sodB at 1733.702 kb on + strand, within sodB at 1733.803 kb on - strand, within sodB at 1733.803 kb on - strand, within sodB at 1734.037 kb on - strand at 1734.128 kb on - strand at 1734.128 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 1,731,640 - -0.7 1,731,728 - +0.4 1,732,385 - +1.6 1,733,172 + ydhO b1655 0.87 -0.3 1,733,172 + ydhO b1655 0.87 -1.2 1,733,225 + +0.1 1,733,425 + +1.5 1,733,522 + sodB b1656 0.21 +0.3 1,733,612 + sodB b1656 0.36 -1.3 1,733,702 + sodB b1656 0.52 -0.5 1,733,803 - sodB b1656 0.69 -0.4 1,733,803 - sodB b1656 0.69 +0.4 1,734,037 - +1.0 1,734,128 - -0.5 1,734,128 - -0.2
Or see this region's nucleotide sequence