Strain Fitness in Escherichia coli BW25113 around b1586

Experiment: 3-methyl-1-butanol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntspeG and ynfC are separated by 2 nucleotidesynfC and b1586 are separated by 107 nucleotidesb1586 and ynfE are separated by 198 nucleotides b1584: speG - spermidine N1-acetyltransferase (NCBI), at 1,654,208 to 1,654,768 speG b1585: ynfC - orf, hypothetical protein (VIMSS), at 1,654,771 to 1,655,481 ynfC b1586: b1586 - orf, hypothetical protein (VIMSS), at 1,655,589 to 1,655,894 b1586 b1587: ynfE - oxidoreductase subunit (NCBI), at 1,656,093 to 1,658,519 ynfE Position (kb) 1655 1656Strain fitness (log2 ratio) -2 -1 0 1 2at 1654.690 kb on + strand, within speGat 1654.690 kb on + strand, within speGat 1654.711 kb on + strand, within speGat 1654.711 kb on + strand, within speGat 1654.719 kb on - strandat 1654.723 kb on + strandat 1654.723 kb on + strandat 1654.731 kb on - strandat 1654.745 kb on + strandat 1654.745 kb on + strandat 1654.753 kb on - strandat 1654.799 kb on + strandat 1654.807 kb on - strandat 1654.807 kb on - strandat 1654.812 kb on - strandat 1654.812 kb on - strandat 1655.022 kb on - strand, within ynfCat 1655.058 kb on + strand, within ynfCat 1655.077 kb on - strand, within ynfCat 1655.077 kb on - strand, within ynfCat 1655.102 kb on + strand, within ynfCat 1655.114 kb on + strand, within ynfCat 1655.123 kb on - strand, within ynfCat 1655.123 kb on - strand, within ynfCat 1655.147 kb on - strand, within ynfCat 1655.175 kb on - strand, within ynfCat 1655.199 kb on - strand, within ynfCat 1655.208 kb on + strand, within ynfCat 1655.216 kb on - strand, within ynfCat 1655.394 kb on + strand, within ynfCat 1655.402 kb on - strandat 1655.402 kb on - strand, within ynfCat 1655.413 kb on - strandat 1655.428 kb on + strandat 1655.500 kb on - strandat 1655.500 kb on - strandat 1655.626 kb on + strand, within b1586at 1655.634 kb on - strand, within b1586at 1655.643 kb on + strand, within b1586at 1655.645 kb on + strand, within b1586at 1655.655 kb on + strand, within b1586at 1655.655 kb on + strand, within b1586at 1655.786 kb on - strand, within b1586at 1655.786 kb on - strand, within b1586at 1655.841 kb on + strand, within b1586at 1655.858 kb on + strand, within b1586at 1655.955 kb on - strandat 1656.025 kb on - strandat 1656.291 kb on - strandat 1656.291 kb on - strandat 1656.418 kb on - strand, within ynfEat 1656.569 kb on - strand, within ynfEat 1656.673 kb on - strand, within ynfEat 1656.712 kb on - strand, within ynfEat 1656.712 kb on - strand, within ynfEat 1656.800 kb on + strand, within ynfEat 1656.800 kb on + strand, within ynfE

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol%
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1,654,690 + speG b1584 0.86 -1.4
1,654,690 + speG b1584 0.86 +1.3
1,654,711 + speG b1584 0.90 -2.0
1,654,711 + speG b1584 0.90 +1.2
1,654,719 - -0.9
1,654,723 + +0.8
1,654,723 + -0.4
1,654,731 - -1.7
1,654,745 + +0.1
1,654,745 + -0.2
1,654,753 - -0.8
1,654,799 + +0.7
1,654,807 - +0.4
1,654,807 - +0.0
1,654,812 - -1.3
1,654,812 - +0.6
1,655,022 - ynfC b1585 0.35 +0.1
1,655,058 + ynfC b1585 0.40 -0.4
1,655,077 - ynfC b1585 0.43 +1.7
1,655,077 - ynfC b1585 0.43 +2.6
1,655,102 + ynfC b1585 0.47 -1.4
1,655,114 + ynfC b1585 0.48 -0.9
1,655,123 - ynfC b1585 0.50 -0.1
1,655,123 - ynfC b1585 0.50 -1.6
1,655,147 - ynfC b1585 0.53 +1.0
1,655,175 - ynfC b1585 0.57 -0.1
1,655,199 - ynfC b1585 0.60 +0.9
1,655,208 + ynfC b1585 0.61 -0.9
1,655,216 - ynfC b1585 0.63 +1.8
1,655,394 + ynfC b1585 0.88 -0.2
1,655,402 - +2.7
1,655,402 - ynfC b1585 0.89 -0.1
1,655,413 - -0.5
1,655,428 + +0.7
1,655,500 - -0.2
1,655,500 - +1.7
1,655,626 + b1586 b1586 0.12 +0.5
1,655,634 - b1586 b1586 0.15 +1.3
1,655,643 + b1586 b1586 0.18 +0.2
1,655,645 + b1586 b1586 0.18 +0.6
1,655,655 + b1586 b1586 0.22 +2.4
1,655,655 + b1586 b1586 0.22 -0.9
1,655,786 - b1586 b1586 0.64 -0.0
1,655,786 - b1586 b1586 0.64 +2.3
1,655,841 + b1586 b1586 0.82 -0.2
1,655,858 + b1586 b1586 0.88 +0.4
1,655,955 - +1.6
1,656,025 - -2.4
1,656,291 - -0.3
1,656,291 - -0.1
1,656,418 - ynfE b1587 0.13 +0.9
1,656,569 - ynfE b1587 0.20 +1.5
1,656,673 - ynfE b1587 0.24 +0.9
1,656,712 - ynfE b1587 0.26 +1.9
1,656,712 - ynfE b1587 0.26 -0.1
1,656,800 + ynfE b1587 0.29 +1.7
1,656,800 + ynfE b1587 0.29 -1.6

Or see this region's nucleotide sequence