Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ssuD and ycbO overlap by 4 nucleotides ycbO and ssuE overlap by 8 nucleotides ssuE and ycbQ are separated by 355 nucleotides
b0935: ssuD - alkanesulfonate monooxygenase (NCBI), at 994,066 to 995,211
ssuD
b0936: ycbO - orf, hypothetical protein (VIMSS), at 995,208 to 996,167
ycbO
b0937: ssuE - NAD(P)H-dependent FMN reductase (NCBI), at 996,160 to 996,735
ssuE
b0938: ycbQ - putative fimbrial-like protein (VIMSS), at 997,091 to 997,630
ycbQ
Position (kb) 995
996
997 Strain fitness (log2 ratio) -2
-1
0
1
2 at 994.335 kb on - strand, within ssuD at 994.356 kb on + strand, within ssuD at 994.364 kb on - strand, within ssuD at 994.597 kb on - strand, within ssuD at 994.710 kb on - strand, within ssuD at 994.710 kb on - strand, within ssuD at 994.723 kb on + strand, within ssuD at 994.869 kb on + strand, within ssuD at 994.869 kb on - strand, within ssuD at 994.900 kb on - strand, within ssuD at 994.900 kb on - strand, within ssuD at 994.962 kb on + strand, within ssuD at 995.043 kb on - strand, within ssuD at 995.236 kb on - strand at 995.236 kb on - strand at 995.473 kb on - strand, within ycbO at 995.512 kb on + strand, within ycbO at 995.607 kb on + strand, within ycbO at 995.616 kb on + strand, within ycbO at 995.631 kb on + strand, within ycbO at 995.734 kb on - strand, within ycbO at 995.753 kb on - strand, within ycbO at 995.832 kb on - strand, within ycbO at 995.871 kb on + strand, within ycbO at 995.871 kb on + strand, within ycbO at 996.185 kb on + strand at 996.207 kb on - strand at 996.267 kb on + strand, within ssuE at 996.350 kb on - strand, within ssuE at 996.637 kb on + strand, within ssuE at 996.637 kb on + strand, within ssuE at 996.710 kb on + strand at 996.710 kb on + strand at 996.714 kb on + strand at 996.718 kb on - strand at 996.721 kb on + strand at 996.721 kb on + strand at 996.722 kb on - strand at 996.743 kb on + strand at 996.743 kb on + strand at 996.751 kb on - strand at 996.774 kb on + strand at 996.793 kb on + strand at 996.793 kb on + strand at 996.797 kb on + strand at 996.797 kb on + strand at 997.121 kb on + strand at 997.152 kb on - strand, within ycbQ at 997.152 kb on - strand, within ycbQ
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 994,335 - ssuD b0935 0.23 -1.5 994,356 + ssuD b0935 0.25 -1.5 994,364 - ssuD b0935 0.26 -2.0 994,597 - ssuD b0935 0.46 +0.9 994,710 - ssuD b0935 0.56 +2.1 994,710 - ssuD b0935 0.56 -1.6 994,723 + ssuD b0935 0.57 +0.6 994,869 + ssuD b0935 0.70 -0.8 994,869 - ssuD b0935 0.70 +0.6 994,900 - ssuD b0935 0.73 +1.8 994,900 - ssuD b0935 0.73 -1.9 994,962 + ssuD b0935 0.78 +0.4 995,043 - ssuD b0935 0.85 -0.0 995,236 - +2.5 995,236 - +1.0 995,473 - ycbO b0936 0.28 -0.3 995,512 + ycbO b0936 0.32 -0.6 995,607 + ycbO b0936 0.42 +0.8 995,616 + ycbO b0936 0.42 -1.0 995,631 + ycbO b0936 0.44 +0.2 995,734 - ycbO b0936 0.55 +0.3 995,753 - ycbO b0936 0.57 -2.2 995,832 - ycbO b0936 0.65 -1.2 995,871 + ycbO b0936 0.69 -1.5 995,871 + ycbO b0936 0.69 -0.2 996,185 + +0.5 996,207 - +0.6 996,267 + ssuE b0937 0.19 -0.1 996,350 - ssuE b0937 0.33 +0.7 996,637 + ssuE b0937 0.83 +1.0 996,637 + ssuE b0937 0.83 -0.7 996,710 + -0.2 996,710 + -1.0 996,714 + +1.8 996,718 - -0.3 996,721 + -0.1 996,721 + -1.2 996,722 - -1.1 996,743 + +0.4 996,743 + -1.7 996,751 - -0.1 996,774 + -0.3 996,793 + +0.4 996,793 + +0.7 996,797 + +0.4 996,797 + -0.1 997,121 + +0.3 997,152 - ycbQ b0938 0.11 -1.5 997,152 - ycbQ b0938 0.11 -0.3
Or see this region's nucleotide sequence