Strain Fitness in Escherichia coli BW25113 around b0228

Experiment: 3-methyl-1-butanol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyafL and yafM are separated by 175 nucleotidesyafM and lfhA are separated by 223 nucleotides b0227: yafL - predicted lipoprotein and C40 family peptidase (NCBI), at 246,712 to 247,461 yafL b0228: yafM - hypothetical protein (NCBI), at 247,637 to 248,134 yafM b0229: lfhA - flagellar biosynthesis (VIMSS), at 248,358 to 250,070 lfhA Position (kb) 247 248 249Strain fitness (log2 ratio) -2 -1 0 1 2 3at 246.742 kb on - strandat 246.821 kb on - strand, within yafLat 246.854 kb on + strand, within yafLat 246.859 kb on + strand, within yafLat 247.129 kb on - strand, within yafLat 247.129 kb on - strand, within yafLat 247.227 kb on - strand, within yafLat 247.227 kb on - strand, within yafLat 247.288 kb on + strand, within yafLat 247.312 kb on - strand, within yafLat 247.409 kb on - strandat 247.493 kb on + strandat 247.502 kb on - strandat 247.729 kb on + strand, within yafMat 247.786 kb on - strand, within yafMat 247.786 kb on - strand, within yafMat 247.797 kb on - strand, within yafMat 247.824 kb on - strand, within yafMat 247.831 kb on - strand, within yafMat 248.035 kb on + strand, within yafMat 248.067 kb on + strand, within yafMat 248.185 kb on - strandat 248.185 kb on - strandat 248.336 kb on + strandat 248.337 kb on - strandat 248.479 kb on + strandat 248.479 kb on + strandat 248.479 kb on - strandat 248.500 kb on + strandat 248.539 kb on - strand, within lfhAat 248.579 kb on + strand, within lfhAat 248.579 kb on + strand, within lfhAat 248.594 kb on + strand, within lfhAat 248.609 kb on + strand, within lfhAat 248.666 kb on - strand, within lfhAat 248.911 kb on + strand, within lfhAat 248.977 kb on - strand, within lfhAat 249.077 kb on + strand, within lfhAat 249.103 kb on - strand, within lfhA

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol%
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246,742 - -2.1
246,821 - yafL b0227 0.15 +0.1
246,854 + yafL b0227 0.19 +0.2
246,859 + yafL b0227 0.20 +0.6
247,129 - yafL b0227 0.56 +0.0
247,129 - yafL b0227 0.56 +3.4
247,227 - yafL b0227 0.69 -1.5
247,227 - yafL b0227 0.69 +1.4
247,288 + yafL b0227 0.77 -0.8
247,312 - yafL b0227 0.80 -0.5
247,409 - +0.6
247,493 + -1.7
247,502 - -1.2
247,729 + yafM b0228 0.18 +2.2
247,786 - yafM b0228 0.30 +1.2
247,786 - yafM b0228 0.30 +2.4
247,797 - yafM b0228 0.32 -0.7
247,824 - yafM b0228 0.38 +1.2
247,831 - yafM b0228 0.39 +0.8
248,035 + yafM b0228 0.80 -0.4
248,067 + yafM b0228 0.86 +0.2
248,185 - +0.5
248,185 - -0.5
248,336 + +0.4
248,337 - +1.1
248,479 + +0.1
248,479 + -0.7
248,479 - +2.1
248,500 + +0.3
248,539 - lfhA b0229 0.11 +0.0
248,579 + lfhA b0229 0.13 -0.3
248,579 + lfhA b0229 0.13 -0.9
248,594 + lfhA b0229 0.14 -0.4
248,609 + lfhA b0229 0.15 +0.6
248,666 - lfhA b0229 0.18 -1.1
248,911 + lfhA b0229 0.32 +0.4
248,977 - lfhA b0229 0.36 +0.9
249,077 + lfhA b0229 0.42 -0.1
249,103 - lfhA b0229 0.43 -1.6

Or see this region's nucleotide sequence