Strain Fitness in Escherichia coli BW25113 around b0111

Experiment: 3-methyl-1-butanol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnadC and ampD are separated by 87 nucleotidesampD and ampE overlap by 4 nucleotidesampE and aroP are separated by 42 nucleotides b0109: nadC - nicotinate-nucleotide pyrophosphorylase (NCBI), at 117,752 to 118,645 nadC b0110: ampD - N-acetyl-anhydromuranmyl-L-alanine amidase (NCBI), at 118,733 to 119,284 ampD b0111: ampE - predicted inner membrane protein (NCBI), at 119,281 to 120,135 ampE b0112: aroP - aromatic amino acid transporter (NCBI), at 120,178 to 121,551 aroP Position (kb) 119 120 121Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 118.296 kb on + strand, within nadCat 118.355 kb on - strand, within nadCat 118.355 kb on - strand, within nadCat 118.364 kb on + strand, within nadCat 118.382 kb on - strand, within nadCat 118.538 kb on + strand, within nadCat 118.623 kb on + strandat 118.631 kb on - strandat 118.737 kb on + strandat 118.737 kb on + strandat 118.781 kb on + strandat 118.836 kb on + strand, within ampDat 118.868 kb on + strand, within ampDat 118.868 kb on + strand, within ampDat 118.904 kb on - strand, within ampDat 119.033 kb on - strand, within ampDat 119.101 kb on + strand, within ampDat 119.109 kb on - strand, within ampDat 119.109 kb on - strand, within ampDat 119.117 kb on - strand, within ampDat 119.119 kb on + strand, within ampDat 119.150 kb on - strand, within ampDat 119.150 kb on - strand, within ampDat 119.163 kb on + strand, within ampDat 119.168 kb on - strand, within ampDat 119.191 kb on + strand, within ampDat 119.191 kb on + strand, within ampDat 119.274 kb on + strandat 119.339 kb on + strandat 119.339 kb on + strandat 119.499 kb on - strand, within ampEat 119.499 kb on - strand, within ampEat 119.522 kb on + strand, within ampEat 119.528 kb on - strand, within ampEat 119.555 kb on - strand, within ampEat 119.555 kb on - strand, within ampEat 119.558 kb on - strand, within ampEat 119.567 kb on + strand, within ampEat 119.567 kb on + strand, within ampEat 119.753 kb on - strand, within ampEat 119.755 kb on - strand, within ampEat 119.755 kb on - strand, within ampEat 119.756 kb on + strand, within ampEat 119.772 kb on - strand, within ampEat 119.786 kb on + strand, within ampEat 119.786 kb on + strand, within ampEat 119.799 kb on - strand, within ampEat 119.799 kb on - strand, within ampEat 119.836 kb on - strand, within ampEat 119.836 kb on - strand, within ampEat 119.915 kb on + strand, within ampEat 119.961 kb on + strand, within ampEat 119.961 kb on + strand, within ampEat 119.983 kb on + strand, within ampEat 119.985 kb on - strand, within ampEat 120.006 kb on - strand, within ampEat 120.013 kb on - strand, within ampEat 120.013 kb on - strand, within ampEat 120.014 kb on + strand, within ampEat 120.014 kb on + strand, within ampEat 120.014 kb on + strand, within ampEat 120.014 kb on + strand, within ampEat 120.037 kb on - strand, within ampEat 120.138 kb on - strandat 120.139 kb on + strandat 120.153 kb on - strandat 120.155 kb on + strandat 120.237 kb on - strandat 120.251 kb on - strandat 120.254 kb on + strandat 120.266 kb on - strandat 120.266 kb on - strandat 120.340 kb on - strand, within aroPat 120.362 kb on - strand, within aroPat 120.377 kb on + strand, within aroPat 120.389 kb on - strand, within aroPat 120.433 kb on + strand, within aroPat 120.461 kb on + strand, within aroPat 120.484 kb on + strand, within aroPat 120.502 kb on - strand, within aroPat 120.502 kb on - strand, within aroPat 120.514 kb on - strand, within aroPat 120.514 kb on - strand, within aroPat 120.518 kb on + strand, within aroPat 120.560 kb on - strand, within aroPat 120.571 kb on - strand, within aroPat 120.611 kb on - strand, within aroPat 120.611 kb on - strand, within aroPat 120.647 kb on - strand, within aroPat 120.647 kb on - strand, within aroPat 120.675 kb on + strand, within aroPat 120.683 kb on - strand, within aroPat 120.706 kb on + strand, within aroPat 120.712 kb on + strand, within aroPat 120.712 kb on + strand, within aroPat 120.752 kb on + strand, within aroPat 120.753 kb on - strand, within aroPat 120.753 kb on - strand, within aroPat 120.847 kb on + strand, within aroPat 120.854 kb on - strand, within aroPat 120.905 kb on - strand, within aroPat 120.905 kb on - strand, within aroPat 120.956 kb on - strand, within aroPat 120.956 kb on - strand, within aroPat 121.032 kb on - strand, within aroPat 121.032 kb on - strand, within aroPat 121.032 kb on - strand, within aroPat 121.054 kb on + strand, within aroPat 121.087 kb on + strand, within aroPat 121.095 kb on - strand, within aroPat 121.128 kb on - strand, within aroPat 121.128 kb on - strand, within aroP

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol%
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118,296 + nadC b0109 0.61 -0.2
118,355 - nadC b0109 0.67 +0.5
118,355 - nadC b0109 0.67 +2.2
118,364 + nadC b0109 0.68 +0.2
118,382 - nadC b0109 0.70 -1.9
118,538 + nadC b0109 0.88 +0.1
118,623 + +0.4
118,631 - -0.8
118,737 + +0.8
118,737 + +0.3
118,781 + +0.3
118,836 + ampD b0110 0.19 +2.2
118,868 + ampD b0110 0.24 +1.6
118,868 + ampD b0110 0.24 +0.5
118,904 - ampD b0110 0.31 -0.3
119,033 - ampD b0110 0.54 -0.1
119,101 + ampD b0110 0.67 +2.2
119,109 - ampD b0110 0.68 +0.5
119,109 - ampD b0110 0.68 +1.0
119,117 - ampD b0110 0.70 +0.2
119,119 + ampD b0110 0.70 +3.1
119,150 - ampD b0110 0.76 +1.5
119,150 - ampD b0110 0.76 +0.2
119,163 + ampD b0110 0.78 -0.3
119,168 - ampD b0110 0.79 -2.0
119,191 + ampD b0110 0.83 +0.9
119,191 + ampD b0110 0.83 -0.3
119,274 + -1.1
119,339 + +0.9
119,339 + -1.5
119,499 - ampE b0111 0.25 +0.4
119,499 - ampE b0111 0.25 -1.3
119,522 + ampE b0111 0.28 -3.0
119,528 - ampE b0111 0.29 -1.1
119,555 - ampE b0111 0.32 -2.0
119,555 - ampE b0111 0.32 +0.3
119,558 - ampE b0111 0.32 -0.5
119,567 + ampE b0111 0.33 -0.1
119,567 + ampE b0111 0.33 -2.8
119,753 - ampE b0111 0.55 -2.9
119,755 - ampE b0111 0.55 -0.0
119,755 - ampE b0111 0.55 +0.9
119,756 + ampE b0111 0.56 +0.2
119,772 - ampE b0111 0.57 -2.0
119,786 + ampE b0111 0.59 -0.3
119,786 + ampE b0111 0.59 -1.3
119,799 - ampE b0111 0.61 +0.9
119,799 - ampE b0111 0.61 +0.1
119,836 - ampE b0111 0.65 -1.8
119,836 - ampE b0111 0.65 -1.6
119,915 + ampE b0111 0.74 +0.9
119,961 + ampE b0111 0.80 +1.1
119,961 + ampE b0111 0.80 +1.9
119,983 + ampE b0111 0.82 +0.4
119,985 - ampE b0111 0.82 -0.3
120,006 - ampE b0111 0.85 +0.7
120,013 - ampE b0111 0.86 +0.7
120,013 - ampE b0111 0.86 -0.7
120,014 + ampE b0111 0.86 +1.3
120,014 + ampE b0111 0.86 -1.9
120,014 + ampE b0111 0.86 -0.3
120,014 + ampE b0111 0.86 -0.0
120,037 - ampE b0111 0.88 -0.0
120,138 - -0.7
120,139 + +0.7
120,153 - -2.3
120,155 + +0.8
120,237 - -0.5
120,251 - -0.7
120,254 + +0.5
120,266 - -2.5
120,266 - +1.4
120,340 - aroP b0112 0.12 -0.8
120,362 - aroP b0112 0.13 +1.5
120,377 + aroP b0112 0.14 -1.1
120,389 - aroP b0112 0.15 -0.3
120,433 + aroP b0112 0.19 +3.9
120,461 + aroP b0112 0.21 +0.2
120,484 + aroP b0112 0.22 -1.4
120,502 - aroP b0112 0.24 +1.3
120,502 - aroP b0112 0.24 +1.4
120,514 - aroP b0112 0.24 -0.5
120,514 - aroP b0112 0.24 -0.7
120,518 + aroP b0112 0.25 +0.9
120,560 - aroP b0112 0.28 -3.0
120,571 - aroP b0112 0.29 +0.5
120,611 - aroP b0112 0.32 -2.2
120,611 - aroP b0112 0.32 +1.9
120,647 - aroP b0112 0.34 +0.3
120,647 - aroP b0112 0.34 -0.1
120,675 + aroP b0112 0.36 -0.1
120,683 - aroP b0112 0.37 -1.1
120,706 + aroP b0112 0.38 +0.3
120,712 + aroP b0112 0.39 +0.3
120,712 + aroP b0112 0.39 -0.5
120,752 + aroP b0112 0.42 +2.4
120,753 - aroP b0112 0.42 -1.4
120,753 - aroP b0112 0.42 -1.8
120,847 + aroP b0112 0.49 -1.5
120,854 - aroP b0112 0.49 +0.3
120,905 - aroP b0112 0.53 -1.7
120,905 - aroP b0112 0.53 -0.9
120,956 - aroP b0112 0.57 -0.5
120,956 - aroP b0112 0.57 +0.3
121,032 - aroP b0112 0.62 +0.8
121,032 - aroP b0112 0.62 +0.4
121,032 - aroP b0112 0.62 +0.2
121,054 + aroP b0112 0.64 +0.3
121,087 + aroP b0112 0.66 -0.3
121,095 - aroP b0112 0.67 -0.7
121,128 - aroP b0112 0.69 +1.2
121,128 - aroP b0112 0.69 -0.7

Or see this region's nucleotide sequence