Strain Fitness in Escherichia coli BW25113 around b0061

Experiment: 3-methyl-1-butanol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpolB and araD are separated by 74 nucleotidesaraD and araA are separated by 284 nucleotides b0060: polB - DNA polymerase II (NCBI), at 63,429 to 65,780 polB b0061: araD - L-ribulose-5-phosphate 4-epimerase (NCBI), at 65,855 to 66,550 araD b0062: araA - L-arabinose isomerase (NCBI), at 66,835 to 68,337 araA Position (kb) 65 66 67Strain fitness (log2 ratio) -2 -1 0 1 2at 65.051 kb on + strand, within polBat 65.172 kb on - strand, within polBat 65.190 kb on + strand, within polBat 65.190 kb on + strand, within polBat 65.278 kb on + strand, within polBat 65.278 kb on + strand, within polBat 65.286 kb on - strand, within polBat 65.291 kb on - strand, within polBat 65.291 kb on - strand, within polBat 65.315 kb on + strand, within polBat 65.317 kb on + strand, within polBat 65.334 kb on - strand, within polBat 65.398 kb on + strand, within polBat 65.406 kb on - strand, within polBat 65.411 kb on + strand, within polBat 65.427 kb on - strand, within polBat 65.482 kb on - strand, within polBat 65.486 kb on - strand, within polBat 65.493 kb on - strand, within polBat 65.493 kb on - strand, within polBat 65.541 kb on + strand, within polBat 65.541 kb on + strand, within polBat 65.652 kb on - strandat 65.692 kb on - strandat 65.814 kb on - strandat 65.816 kb on + strandat 65.824 kb on - strandat 65.865 kb on + strandat 65.873 kb on - strandat 65.873 kb on - strandat 65.883 kb on + strandat 65.883 kb on + strandat 65.891 kb on - strandat 65.900 kb on - strandat 65.949 kb on + strand, within araDat 65.949 kb on + strand, within araDat 65.977 kb on + strand, within araDat 66.057 kb on - strand, within araDat 66.057 kb on - strand, within araDat 66.195 kb on + strand, within araDat 66.226 kb on + strand, within araDat 66.226 kb on + strand, within araDat 66.271 kb on - strand, within araDat 66.271 kb on - strand, within araDat 66.276 kb on - strand, within araDat 66.381 kb on + strand, within araDat 66.381 kb on + strand, within araDat 66.470 kb on + strand, within araDat 66.470 kb on + strand, within araDat 66.473 kb on - strand, within araDat 66.473 kb on - strand, within araDat 66.483 kb on - strandat 66.484 kb on + strandat 66.484 kb on + strandat 66.527 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol%
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65,051 + polB b0060 0.69 -1.9
65,172 - polB b0060 0.74 -0.4
65,190 + polB b0060 0.75 -1.7
65,190 + polB b0060 0.75 +1.9
65,278 + polB b0060 0.79 -1.8
65,278 + polB b0060 0.79 +0.4
65,286 - polB b0060 0.79 -1.1
65,291 - polB b0060 0.79 -0.5
65,291 - polB b0060 0.79 -1.5
65,315 + polB b0060 0.80 +0.5
65,317 + polB b0060 0.80 -0.7
65,334 - polB b0060 0.81 -1.6
65,398 + polB b0060 0.84 +2.1
65,406 - polB b0060 0.84 -0.6
65,411 + polB b0060 0.84 -0.1
65,427 - polB b0060 0.85 -0.8
65,482 - polB b0060 0.87 +0.1
65,486 - polB b0060 0.87 -0.5
65,493 - polB b0060 0.88 -1.8
65,493 - polB b0060 0.88 -0.7
65,541 + polB b0060 0.90 +0.7
65,541 + polB b0060 0.90 -0.0
65,652 - -2.5
65,692 - -0.9
65,814 - -1.6
65,816 + -2.2
65,824 - -0.3
65,865 + -1.5
65,873 - -1.3
65,873 - +1.9
65,883 + +1.9
65,883 + -0.1
65,891 - +0.5
65,900 - -0.2
65,949 + araD b0061 0.14 -0.3
65,949 + araD b0061 0.14 -0.3
65,977 + araD b0061 0.18 -0.0
66,057 - araD b0061 0.29 -0.9
66,057 - araD b0061 0.29 -0.1
66,195 + araD b0061 0.49 -1.3
66,226 + araD b0061 0.53 +1.2
66,226 + araD b0061 0.53 -1.3
66,271 - araD b0061 0.60 -1.1
66,271 - araD b0061 0.60 -0.5
66,276 - araD b0061 0.60 +1.7
66,381 + araD b0061 0.76 -1.3
66,381 + araD b0061 0.76 -0.7
66,470 + araD b0061 0.88 +1.8
66,470 + araD b0061 0.88 -2.5
66,473 - araD b0061 0.89 -0.3
66,473 - araD b0061 0.89 -2.7
66,483 - +1.0
66,484 + +1.9
66,484 + +1.9
66,527 - -1.8

Or see this region's nucleotide sequence