Experiment: KB with capsaicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3818 and Psyr_3819 are separated by 56 nucleotides Psyr_3819 and Psyr_3820 are separated by 443 nucleotides
Psyr_3818: Psyr_3818 - conserved hypothetical protein, at 4,543,818 to 4,544,201
_3818
Psyr_3819: Psyr_3819 - Protein of unknown function DUF1349, at 4,544,258 to 4,544,833
_3819
Psyr_3820: Psyr_3820 - conserved domain protein, at 4,545,277 to 4,545,822
_3820
Position (kb)
4544
4545 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4543.325 kb on - strand at 4543.334 kb on + strand at 4543.335 kb on - strand at 4543.335 kb on - strand at 4543.452 kb on + strand at 4543.453 kb on - strand at 4543.528 kb on + strand at 4543.544 kb on + strand at 4543.582 kb on + strand at 4543.643 kb on + strand at 4543.713 kb on + strand at 4543.719 kb on + strand at 4543.719 kb on + strand at 4543.719 kb on + strand at 4543.719 kb on + strand at 4543.719 kb on + strand at 4543.725 kb on + strand at 4543.726 kb on - strand at 4543.729 kb on + strand at 4543.730 kb on - strand at 4543.796 kb on + strand at 4543.796 kb on + strand at 4543.796 kb on + strand at 4543.797 kb on - strand at 4543.797 kb on - strand at 4544.025 kb on + strand, within Psyr_3818 at 4544.025 kb on + strand, within Psyr_3818 at 4544.026 kb on - strand, within Psyr_3818 at 4544.026 kb on - strand, within Psyr_3818 at 4544.145 kb on + strand, within Psyr_3818 at 4544.146 kb on - strand, within Psyr_3818 at 4544.147 kb on + strand, within Psyr_3818 at 4544.147 kb on + strand, within Psyr_3818 at 4544.209 kb on + strand at 4544.209 kb on + strand at 4544.210 kb on - strand at 4544.360 kb on + strand, within Psyr_3819 at 4544.360 kb on + strand, within Psyr_3819 at 4544.360 kb on + strand, within Psyr_3819 at 4544.361 kb on - strand, within Psyr_3819 at 4544.361 kb on - strand, within Psyr_3819 at 4544.361 kb on - strand, within Psyr_3819 at 4544.361 kb on - strand, within Psyr_3819 at 4544.361 kb on - strand, within Psyr_3819 at 4544.365 kb on + strand, within Psyr_3819 at 4544.365 kb on + strand, within Psyr_3819 at 4544.365 kb on + strand, within Psyr_3819 at 4544.366 kb on - strand, within Psyr_3819 at 4544.366 kb on - strand, within Psyr_3819 at 4544.368 kb on - strand, within Psyr_3819 at 4544.368 kb on - strand, within Psyr_3819 at 4544.444 kb on + strand, within Psyr_3819 at 4544.498 kb on + strand, within Psyr_3819 at 4544.498 kb on + strand, within Psyr_3819 at 4544.499 kb on - strand, within Psyr_3819 at 4544.531 kb on + strand, within Psyr_3819 at 4544.629 kb on - strand, within Psyr_3819 at 4544.629 kb on - strand, within Psyr_3819 at 4544.629 kb on - strand, within Psyr_3819 at 4544.877 kb on + strand at 4544.877 kb on + strand at 4544.877 kb on + strand at 4544.878 kb on - strand at 4544.878 kb on - strand at 4544.878 kb on - strand at 4544.885 kb on - strand at 4544.895 kb on - strand at 4544.908 kb on + strand at 4544.909 kb on - strand at 4544.909 kb on - strand at 4544.990 kb on - strand at 4545.129 kb on + strand at 4545.129 kb on + strand at 4545.130 kb on - strand at 4545.140 kb on + strand at 4545.141 kb on - strand at 4545.141 kb on - strand at 4545.553 kb on + strand, within Psyr_3820 at 4545.554 kb on - strand, within Psyr_3820
Per-strain Table
Position Strand Gene LocusTag Fraction KB with capsaicin 0.125 mg/ml remove 4,543,325 - +0.2 4,543,334 + -0.6 4,543,335 - +0.3 4,543,335 - -0.2 4,543,452 + -0.2 4,543,453 - -0.1 4,543,528 + -0.8 4,543,544 + +0.8 4,543,582 + +0.9 4,543,643 + +1.0 4,543,713 + +1.2 4,543,719 + +2.0 4,543,719 + +1.4 4,543,719 + +1.8 4,543,719 + -1.9 4,543,719 + -0.1 4,543,725 + -0.2 4,543,726 - +0.8 4,543,729 + -0.1 4,543,730 - -0.8 4,543,796 + +0.3 4,543,796 + +0.6 4,543,796 + +1.3 4,543,797 - -0.6 4,543,797 - -0.2 4,544,025 + Psyr_3818 0.54 +1.6 4,544,025 + Psyr_3818 0.54 -0.0 4,544,026 - Psyr_3818 0.54 +1.0 4,544,026 - Psyr_3818 0.54 -1.2 4,544,145 + Psyr_3818 0.85 +0.4 4,544,146 - Psyr_3818 0.85 -0.4 4,544,147 + Psyr_3818 0.86 +0.3 4,544,147 + Psyr_3818 0.86 +0.4 4,544,209 + +0.2 4,544,209 + -0.4 4,544,210 - -0.9 4,544,360 + Psyr_3819 0.18 -0.6 4,544,360 + Psyr_3819 0.18 -0.4 4,544,360 + Psyr_3819 0.18 +1.3 4,544,361 - Psyr_3819 0.18 -1.0 4,544,361 - Psyr_3819 0.18 -0.5 4,544,361 - Psyr_3819 0.18 +2.0 4,544,361 - Psyr_3819 0.18 +1.0 4,544,361 - Psyr_3819 0.18 +0.3 4,544,365 + Psyr_3819 0.19 +0.4 4,544,365 + Psyr_3819 0.19 +0.0 4,544,365 + Psyr_3819 0.19 -0.8 4,544,366 - Psyr_3819 0.19 -1.8 4,544,366 - Psyr_3819 0.19 -1.2 4,544,368 - Psyr_3819 0.19 -0.6 4,544,368 - Psyr_3819 0.19 -0.2 4,544,444 + Psyr_3819 0.32 +0.3 4,544,498 + Psyr_3819 0.42 -0.2 4,544,498 + Psyr_3819 0.42 -1.9 4,544,499 - Psyr_3819 0.42 +0.3 4,544,531 + Psyr_3819 0.47 -2.2 4,544,629 - Psyr_3819 0.64 -1.8 4,544,629 - Psyr_3819 0.64 +0.6 4,544,629 - Psyr_3819 0.64 -0.9 4,544,877 + +0.0 4,544,877 + -1.3 4,544,877 + +0.9 4,544,878 - -1.0 4,544,878 - +0.5 4,544,878 - +0.3 4,544,885 - +0.6 4,544,895 - +0.1 4,544,908 + +0.5 4,544,909 - +0.9 4,544,909 - -0.2 4,544,990 - -0.1 4,545,129 + +0.3 4,545,129 + +0.0 4,545,130 - +0.6 4,545,140 + -0.6 4,545,141 - +0.8 4,545,141 - -0.7 4,545,553 + Psyr_3820 0.51 -0.0 4,545,554 - Psyr_3820 0.51 +1.9
Or see this region's nucleotide sequence