Experiment: KB with capsaicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0497 and Psyr_0498 are separated by 2 nucleotides Psyr_0498 and Psyr_0499 are separated by 9 nucleotides Psyr_0499 and Psyr_0500 overlap by 4 nucleotides
Psyr_0497: Psyr_0497 - LamB/YcsF, at 539,323 to 540,087
_0497
Psyr_0498: Psyr_0498 - Protein of unknown function DUF1445, at 540,090 to 540,884
_0498
Psyr_0499: Psyr_0499 - Urea amidolyase-related protein, at 540,894 to 542,498
_0499
Psyr_0500: Psyr_0500 - biotin carboxyl carrier protein / biotin carboxylase, at 542,495 to 544,228
_0500
Position (kb) 540
541
542
543 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 540.229 kb on - strand, within Psyr_0498 at 540.229 kb on - strand, within Psyr_0498 at 540.229 kb on - strand, within Psyr_0498 at 540.870 kb on + strand at 540.870 kb on + strand at 540.871 kb on - strand at 540.871 kb on - strand at 540.871 kb on - strand at 540.873 kb on + strand at 540.874 kb on - strand at 540.880 kb on + strand at 540.880 kb on + strand at 540.880 kb on + strand at 540.880 kb on + strand at 540.880 kb on + strand at 540.880 kb on + strand at 540.880 kb on + strand at 540.881 kb on - strand at 540.881 kb on - strand at 540.881 kb on - strand at 540.881 kb on - strand at 540.881 kb on - strand at 540.881 kb on - strand at 540.881 kb on - strand at 540.881 kb on - strand at 540.881 kb on - strand at 540.881 kb on - strand at 540.882 kb on + strand at 540.883 kb on - strand at 540.883 kb on - strand at 540.883 kb on - strand at 540.883 kb on - strand at 540.883 kb on - strand at 541.138 kb on + strand, within Psyr_0499 at 541.149 kb on + strand, within Psyr_0499 at 541.149 kb on + strand, within Psyr_0499 at 541.149 kb on + strand, within Psyr_0499 at 541.149 kb on + strand, within Psyr_0499 at 541.149 kb on + strand, within Psyr_0499 at 541.149 kb on + strand, within Psyr_0499 at 541.149 kb on + strand, within Psyr_0499 at 541.149 kb on + strand, within Psyr_0499 at 541.149 kb on + strand, within Psyr_0499 at 541.150 kb on - strand, within Psyr_0499 at 541.150 kb on - strand, within Psyr_0499 at 541.150 kb on - strand, within Psyr_0499 at 541.233 kb on + strand, within Psyr_0499 at 541.234 kb on - strand, within Psyr_0499 at 541.272 kb on + strand, within Psyr_0499 at 541.370 kb on + strand, within Psyr_0499 at 541.371 kb on - strand, within Psyr_0499 at 541.569 kb on + strand, within Psyr_0499 at 541.569 kb on + strand, within Psyr_0499 at 541.569 kb on + strand, within Psyr_0499 at 541.570 kb on - strand, within Psyr_0499 at 541.848 kb on + strand, within Psyr_0499 at 541.917 kb on + strand, within Psyr_0499 at 541.917 kb on + strand, within Psyr_0499 at 542.385 kb on + strand at 542.539 kb on + strand at 542.539 kb on + strand at 542.562 kb on - strand at 542.585 kb on + strand at 542.669 kb on + strand, within Psyr_0500 at 542.669 kb on + strand, within Psyr_0500 at 542.669 kb on + strand, within Psyr_0500 at 542.670 kb on - strand, within Psyr_0500 at 542.670 kb on - strand, within Psyr_0500 at 542.670 kb on - strand, within Psyr_0500 at 542.670 kb on - strand, within Psyr_0500 at 542.670 kb on - strand, within Psyr_0500 at 542.854 kb on + strand, within Psyr_0500 at 543.227 kb on + strand, within Psyr_0500 at 543.293 kb on + strand, within Psyr_0500
Per-strain Table
Position Strand Gene LocusTag Fraction KB with capsaicin 0.125 mg/ml remove 540,229 - Psyr_0498 0.17 -0.1 540,229 - Psyr_0498 0.17 -1.6 540,229 - Psyr_0498 0.17 -1.7 540,870 + +0.4 540,870 + -0.0 540,871 - -0.3 540,871 - -0.3 540,871 - +0.8 540,873 + -1.0 540,874 - +0.4 540,880 + -0.8 540,880 + -0.2 540,880 + -0.2 540,880 + -0.5 540,880 + +1.7 540,880 + -1.5 540,880 + -0.3 540,881 - +0.0 540,881 - +0.4 540,881 - -0.2 540,881 - +0.2 540,881 - -2.3 540,881 - +0.3 540,881 - -0.3 540,881 - -0.3 540,881 - -0.9 540,881 - +0.6 540,882 + -0.8 540,883 - +1.3 540,883 - -0.1 540,883 - +0.9 540,883 - +0.3 540,883 - -0.6 541,138 + Psyr_0499 0.15 +1.1 541,149 + Psyr_0499 0.16 -0.9 541,149 + Psyr_0499 0.16 -2.3 541,149 + Psyr_0499 0.16 +1.2 541,149 + Psyr_0499 0.16 -0.4 541,149 + Psyr_0499 0.16 -0.5 541,149 + Psyr_0499 0.16 +2.8 541,149 + Psyr_0499 0.16 +0.6 541,149 + Psyr_0499 0.16 -0.8 541,149 + Psyr_0499 0.16 +0.5 541,150 - Psyr_0499 0.16 +0.1 541,150 - Psyr_0499 0.16 -0.5 541,150 - Psyr_0499 0.16 +0.5 541,233 + Psyr_0499 0.21 +0.6 541,234 - Psyr_0499 0.21 +0.1 541,272 + Psyr_0499 0.24 +0.4 541,370 + Psyr_0499 0.30 -0.3 541,371 - Psyr_0499 0.30 +0.9 541,569 + Psyr_0499 0.42 -0.1 541,569 + Psyr_0499 0.42 -0.9 541,569 + Psyr_0499 0.42 -1.4 541,570 - Psyr_0499 0.42 +1.5 541,848 + Psyr_0499 0.59 -0.3 541,917 + Psyr_0499 0.64 -0.9 541,917 + Psyr_0499 0.64 +0.3 542,385 + -1.2 542,539 + -0.3 542,539 + +0.4 542,562 - -0.8 542,585 + +0.4 542,669 + Psyr_0500 0.10 -0.1 542,669 + Psyr_0500 0.10 +0.8 542,669 + Psyr_0500 0.10 +0.2 542,670 - Psyr_0500 0.10 -0.3 542,670 - Psyr_0500 0.10 +1.6 542,670 - Psyr_0500 0.10 +0.4 542,670 - Psyr_0500 0.10 -0.2 542,670 - Psyr_0500 0.10 -1.7 542,854 + Psyr_0500 0.21 +0.3 543,227 + Psyr_0500 0.42 +0.2 543,293 + Psyr_0500 0.46 +0.6
Or see this region's nucleotide sequence