Experiment: KB with capsaicin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2407 and Psyr_2408 overlap by 4 nucleotides Psyr_2408 and Psyr_2409 are separated by 34 nucleotides Psyr_2409 and Psyr_2410 are separated by 270 nucleotides
Psyr_2407: Psyr_2407 - aldehyde dehydrogenase (acceptor), at 2,787,944 to 2,789,425
_2407
Psyr_2408: Psyr_2408 - 3-oxoadipate enol-lactonase, at 2,789,422 to 2,790,270
_2408
Psyr_2409: Psyr_2409 - Protein of unknown function DUF1185, at 2,790,305 to 2,790,895
_2409
Psyr_2410: Psyr_2410 - Zinc-containing alcohol dehydrogenase superfamily, at 2,791,166 to 2,792,305
_2410
Position (kb)
2789
2790
2791 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2788.590 kb on + strand, within Psyr_2407 at 2788.590 kb on + strand, within Psyr_2407 at 2788.590 kb on + strand, within Psyr_2407 at 2788.590 kb on + strand, within Psyr_2407 at 2788.590 kb on + strand, within Psyr_2407 at 2788.590 kb on + strand, within Psyr_2407 at 2788.590 kb on + strand, within Psyr_2407 at 2788.591 kb on - strand, within Psyr_2407 at 2788.591 kb on - strand, within Psyr_2407 at 2788.591 kb on - strand, within Psyr_2407 at 2788.748 kb on + strand, within Psyr_2407 at 2788.954 kb on - strand, within Psyr_2407 at 2789.538 kb on + strand, within Psyr_2408 at 2789.538 kb on + strand, within Psyr_2408 at 2789.539 kb on - strand, within Psyr_2408 at 2789.539 kb on - strand, within Psyr_2408 at 2789.539 kb on - strand, within Psyr_2408 at 2789.684 kb on + strand, within Psyr_2408 at 2789.685 kb on - strand, within Psyr_2408 at 2789.688 kb on + strand, within Psyr_2408 at 2789.711 kb on + strand, within Psyr_2408 at 2789.711 kb on + strand, within Psyr_2408 at 2789.711 kb on + strand, within Psyr_2408 at 2789.712 kb on - strand, within Psyr_2408 at 2789.712 kb on - strand, within Psyr_2408 at 2789.723 kb on + strand, within Psyr_2408 at 2789.724 kb on - strand, within Psyr_2408 at 2789.793 kb on - strand, within Psyr_2408 at 2789.924 kb on - strand, within Psyr_2408 at 2790.227 kb on + strand at 2790.228 kb on - strand at 2790.228 kb on - strand at 2790.293 kb on - strand at 2790.345 kb on + strand at 2790.345 kb on + strand at 2790.345 kb on + strand at 2790.346 kb on - strand at 2790.398 kb on + strand, within Psyr_2409 at 2790.399 kb on - strand, within Psyr_2409 at 2790.399 kb on - strand, within Psyr_2409 at 2790.399 kb on - strand, within Psyr_2409 at 2790.399 kb on - strand, within Psyr_2409 at 2790.453 kb on + strand, within Psyr_2409 at 2790.454 kb on - strand, within Psyr_2409 at 2790.454 kb on - strand, within Psyr_2409 at 2790.454 kb on - strand, within Psyr_2409 at 2790.654 kb on + strand, within Psyr_2409 at 2790.864 kb on + strand at 2790.864 kb on + strand at 2790.864 kb on + strand at 2790.865 kb on - strand at 2790.865 kb on - strand at 2791.006 kb on + strand at 2791.034 kb on + strand at 2791.069 kb on - strand at 2791.069 kb on - strand at 2791.111 kb on + strand at 2791.111 kb on + strand at 2791.112 kb on - strand at 2791.181 kb on + strand at 2791.182 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with capsaicin 0.25 mg/ml remove 2,788,590 + Psyr_2407 0.44 -0.6 2,788,590 + Psyr_2407 0.44 +0.3 2,788,590 + Psyr_2407 0.44 +0.2 2,788,590 + Psyr_2407 0.44 -2.1 2,788,590 + Psyr_2407 0.44 -0.3 2,788,590 + Psyr_2407 0.44 +0.9 2,788,590 + Psyr_2407 0.44 -0.6 2,788,591 - Psyr_2407 0.44 -0.1 2,788,591 - Psyr_2407 0.44 -0.1 2,788,591 - Psyr_2407 0.44 -1.7 2,788,748 + Psyr_2407 0.54 -1.1 2,788,954 - Psyr_2407 0.68 -1.2 2,789,538 + Psyr_2408 0.14 +1.3 2,789,538 + Psyr_2408 0.14 +0.8 2,789,539 - Psyr_2408 0.14 +1.2 2,789,539 - Psyr_2408 0.14 +1.0 2,789,539 - Psyr_2408 0.14 -0.4 2,789,684 + Psyr_2408 0.31 +1.5 2,789,685 - Psyr_2408 0.31 +1.0 2,789,688 + Psyr_2408 0.31 +1.2 2,789,711 + Psyr_2408 0.34 +0.0 2,789,711 + Psyr_2408 0.34 +0.2 2,789,711 + Psyr_2408 0.34 +1.8 2,789,712 - Psyr_2408 0.34 +0.3 2,789,712 - Psyr_2408 0.34 +0.5 2,789,723 + Psyr_2408 0.35 +0.7 2,789,724 - Psyr_2408 0.36 +0.5 2,789,793 - Psyr_2408 0.44 +0.1 2,789,924 - Psyr_2408 0.59 -0.4 2,790,227 + -0.5 2,790,228 - -1.6 2,790,228 - +0.7 2,790,293 - +0.8 2,790,345 + -0.1 2,790,345 + +0.1 2,790,345 + +1.2 2,790,346 - +0.7 2,790,398 + Psyr_2409 0.16 +1.1 2,790,399 - Psyr_2409 0.16 -0.7 2,790,399 - Psyr_2409 0.16 +1.2 2,790,399 - Psyr_2409 0.16 -0.6 2,790,399 - Psyr_2409 0.16 +0.8 2,790,453 + Psyr_2409 0.25 -1.7 2,790,454 - Psyr_2409 0.25 -0.9 2,790,454 - Psyr_2409 0.25 +0.6 2,790,454 - Psyr_2409 0.25 +0.3 2,790,654 + Psyr_2409 0.59 +0.5 2,790,864 + -0.1 2,790,864 + -0.5 2,790,864 + +0.4 2,790,865 - +0.5 2,790,865 - -0.8 2,791,006 + -1.2 2,791,034 + -1.3 2,791,069 - -1.1 2,791,069 - +0.4 2,791,111 + +0.7 2,791,111 + +0.5 2,791,112 - -0.4 2,791,181 + -0.9 2,791,182 - +1.0
Or see this region's nucleotide sequence