Experiment: KB with capsaicin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1549 and Psyr_1550 are separated by 0 nucleotides Psyr_1550 and Psyr_1551 are separated by 28 nucleotides Psyr_1551 and Psyr_RNA25 are separated by 92 nucleotides Psyr_RNA25 and Psyr_1552 are separated by 58 nucleotides
Psyr_1549: Psyr_1549 - lipoprotein, putative, at 1,742,241 to 1,743,359
_1549
Psyr_1550: Psyr_1550 - Beta-lactamase-like protein, at 1,743,360 to 1,744,118
_1550
Psyr_1551: Psyr_1551 - phosphoribosylaminoimidazole-succinocarboxamide synthase, at 1,744,147 to 1,744,857
_1551
Psyr_RNA25: Psyr_RNA25 - tRNA-Ser, at 1,744,950 to 1,745,039
_RNA25
Psyr_1552: Psyr_1552 - CDS, at 1,745,098 to 1,745,496
_1552
Position (kb)
1744
1745 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1743.422 kb on + strand at 1743.423 kb on - strand at 1743.426 kb on + strand at 1743.426 kb on + strand at 1743.426 kb on + strand at 1743.426 kb on + strand at 1743.426 kb on + strand at 1743.426 kb on + strand at 1743.426 kb on + strand at 1743.426 kb on + strand at 1743.426 kb on + strand at 1743.426 kb on + strand at 1743.426 kb on + strand at 1743.426 kb on + strand at 1743.426 kb on + strand at 1743.426 kb on + strand at 1743.426 kb on + strand at 1743.427 kb on - strand at 1743.430 kb on + strand at 1743.514 kb on + strand, within Psyr_1550 at 1743.514 kb on + strand, within Psyr_1550 at 1743.588 kb on + strand, within Psyr_1550 at 1743.588 kb on + strand, within Psyr_1550 at 1743.796 kb on + strand, within Psyr_1550 at 1743.796 kb on + strand, within Psyr_1550 at 1743.796 kb on + strand, within Psyr_1550 at 1743.796 kb on + strand, within Psyr_1550 at 1743.796 kb on + strand, within Psyr_1550 at 1743.796 kb on + strand, within Psyr_1550 at 1743.796 kb on + strand, within Psyr_1550 at 1743.799 kb on + strand, within Psyr_1550 at 1743.799 kb on + strand, within Psyr_1550 at 1744.125 kb on + strand at 1744.125 kb on + strand at 1744.129 kb on + strand at 1744.129 kb on + strand at 1744.129 kb on + strand at 1744.856 kb on - strand at 1744.935 kb on - strand at 1744.996 kb on + strand, within Psyr_RNA25 at 1744.997 kb on - strand, within Psyr_RNA25 at 1745.012 kb on + strand, within Psyr_RNA25 at 1745.040 kb on + strand at 1745.046 kb on + strand at 1745.080 kb on + strand at 1745.114 kb on - strand at 1745.187 kb on + strand, within Psyr_1552 at 1745.187 kb on + strand, within Psyr_1552 at 1745.187 kb on + strand, within Psyr_1552 at 1745.188 kb on - strand, within Psyr_1552 at 1745.188 kb on - strand, within Psyr_1552 at 1745.188 kb on - strand, within Psyr_1552 at 1745.188 kb on - strand, within Psyr_1552 at 1745.262 kb on + strand, within Psyr_1552 at 1745.262 kb on + strand, within Psyr_1552 at 1745.263 kb on - strand, within Psyr_1552 at 1745.263 kb on - strand, within Psyr_1552 at 1745.263 kb on - strand, within Psyr_1552 at 1745.269 kb on + strand, within Psyr_1552 at 1745.269 kb on + strand, within Psyr_1552 at 1745.269 kb on + strand, within Psyr_1552 at 1745.269 kb on + strand, within Psyr_1552 at 1745.269 kb on + strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.277 kb on + strand, within Psyr_1552 at 1745.316 kb on + strand, within Psyr_1552 at 1745.317 kb on - strand, within Psyr_1552 at 1745.498 kb on + strand at 1745.498 kb on + strand at 1745.514 kb on - strand at 1745.550 kb on + strand at 1745.550 kb on + strand at 1745.550 kb on + strand at 1745.551 kb on - strand at 1745.551 kb on - strand at 1745.551 kb on - strand at 1745.584 kb on + strand at 1745.613 kb on + strand at 1745.638 kb on + strand at 1745.638 kb on + strand at 1745.639 kb on - strand at 1745.656 kb on - strand at 1745.688 kb on + strand at 1745.784 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with capsaicin 0.25 mg/ml remove 1,743,422 + +0.0 1,743,423 - -0.3 1,743,426 + -0.8 1,743,426 + -1.3 1,743,426 + +1.2 1,743,426 + +1.5 1,743,426 + -0.6 1,743,426 + -0.1 1,743,426 + -0.6 1,743,426 + -0.6 1,743,426 + +0.3 1,743,426 + -0.5 1,743,426 + -0.5 1,743,426 + +1.0 1,743,426 + -1.5 1,743,426 + +0.3 1,743,426 + +0.4 1,743,427 - +0.7 1,743,430 + +0.8 1,743,514 + Psyr_1550 0.20 +0.1 1,743,514 + Psyr_1550 0.20 +1.7 1,743,588 + Psyr_1550 0.30 +1.1 1,743,588 + Psyr_1550 0.30 +1.4 1,743,796 + Psyr_1550 0.57 -0.3 1,743,796 + Psyr_1550 0.57 -0.8 1,743,796 + Psyr_1550 0.57 +0.8 1,743,796 + Psyr_1550 0.57 -0.0 1,743,796 + Psyr_1550 0.57 -0.3 1,743,796 + Psyr_1550 0.57 -0.0 1,743,796 + Psyr_1550 0.57 +1.0 1,743,799 + Psyr_1550 0.58 -0.4 1,743,799 + Psyr_1550 0.58 +0.1 1,744,125 + -0.5 1,744,125 + -0.2 1,744,129 + +3.2 1,744,129 + +0.4 1,744,129 + +0.2 1,744,856 - -0.3 1,744,935 - +0.2 1,744,996 + Psyr_RNA25 0.51 -1.9 1,744,997 - Psyr_RNA25 0.52 -2.8 1,745,012 + Psyr_RNA25 0.69 -1.9 1,745,040 + -0.0 1,745,046 + +1.0 1,745,080 + +0.4 1,745,114 - +1.1 1,745,187 + Psyr_1552 0.22 +0.5 1,745,187 + Psyr_1552 0.22 +0.1 1,745,187 + Psyr_1552 0.22 -0.3 1,745,188 - Psyr_1552 0.23 +1.5 1,745,188 - Psyr_1552 0.23 +0.7 1,745,188 - Psyr_1552 0.23 +0.1 1,745,188 - Psyr_1552 0.23 -1.8 1,745,262 + Psyr_1552 0.41 -0.5 1,745,262 + Psyr_1552 0.41 -0.1 1,745,263 - Psyr_1552 0.41 +0.3 1,745,263 - Psyr_1552 0.41 +0.4 1,745,263 - Psyr_1552 0.41 -0.5 1,745,269 + Psyr_1552 0.43 +0.7 1,745,269 + Psyr_1552 0.43 +1.1 1,745,269 + Psyr_1552 0.43 +0.2 1,745,269 + Psyr_1552 0.43 -0.2 1,745,269 + Psyr_1552 0.43 +0.4 1,745,270 - Psyr_1552 0.43 +0.4 1,745,270 - Psyr_1552 0.43 +0.1 1,745,270 - Psyr_1552 0.43 -0.2 1,745,270 - Psyr_1552 0.43 +1.0 1,745,270 - Psyr_1552 0.43 +2.3 1,745,277 + Psyr_1552 0.45 -0.7 1,745,316 + Psyr_1552 0.55 -0.0 1,745,317 - Psyr_1552 0.55 -0.6 1,745,498 + -1.2 1,745,498 + -0.9 1,745,514 - +0.3 1,745,550 + +0.0 1,745,550 + +0.3 1,745,550 + -0.1 1,745,551 - +0.8 1,745,551 - +0.5 1,745,551 - -0.0 1,745,584 + -0.3 1,745,613 + -0.1 1,745,638 + -0.0 1,745,638 + -1.0 1,745,639 - +0.9 1,745,656 - +0.7 1,745,688 + -0.6 1,745,784 + -0.6
Or see this region's nucleotide sequence