Experiment: KB with capsaicin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1148 and Psyr_1149 are separated by 56 nucleotides Psyr_1149 and Psyr_1150 are separated by 166 nucleotides
Psyr_1148: Psyr_1148 - branched chain amino acid aminotransferase apoenzyme, at 1,299,152 to 1,300,171
_1148
Psyr_1149: Psyr_1149 - conserved hypothetical protein, at 1,300,228 to 1,301,220
_1149
Psyr_1150: Psyr_1150 - chemotaxis sensory transducer, at 1,301,387 to 1,303,042
_1150
Position (kb)
1300
1301
1302 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1299.281 kb on - strand, within Psyr_1148 at 1299.309 kb on - strand, within Psyr_1148 at 1299.525 kb on - strand, within Psyr_1148 at 1299.716 kb on + strand, within Psyr_1148 at 1299.717 kb on - strand, within Psyr_1148 at 1299.776 kb on + strand, within Psyr_1148 at 1299.776 kb on + strand, within Psyr_1148 at 1299.777 kb on - strand, within Psyr_1148 at 1299.777 kb on - strand, within Psyr_1148 at 1299.777 kb on - strand, within Psyr_1148 at 1299.777 kb on - strand, within Psyr_1148 at 1299.777 kb on - strand, within Psyr_1148 at 1300.018 kb on + strand, within Psyr_1148 at 1300.040 kb on + strand, within Psyr_1148 at 1300.040 kb on + strand, within Psyr_1148 at 1300.041 kb on - strand, within Psyr_1148 at 1300.064 kb on + strand, within Psyr_1148 at 1300.064 kb on + strand, within Psyr_1148 at 1300.065 kb on - strand, within Psyr_1148 at 1300.103 kb on + strand at 1300.103 kb on + strand at 1300.103 kb on + strand at 1300.103 kb on + strand at 1300.104 kb on - strand at 1300.104 kb on - strand at 1300.317 kb on + strand at 1300.319 kb on + strand at 1300.319 kb on + strand at 1300.319 kb on + strand at 1300.320 kb on - strand at 1300.320 kb on - strand at 1300.361 kb on + strand, within Psyr_1149 at 1300.362 kb on - strand, within Psyr_1149 at 1300.412 kb on + strand, within Psyr_1149 at 1300.412 kb on + strand, within Psyr_1149 at 1300.413 kb on - strand, within Psyr_1149 at 1300.413 kb on - strand, within Psyr_1149 at 1300.413 kb on - strand, within Psyr_1149 at 1300.454 kb on + strand, within Psyr_1149 at 1300.454 kb on + strand, within Psyr_1149 at 1300.454 kb on + strand, within Psyr_1149 at 1300.575 kb on - strand, within Psyr_1149 at 1300.575 kb on - strand, within Psyr_1149 at 1300.593 kb on - strand, within Psyr_1149 at 1300.682 kb on + strand, within Psyr_1149 at 1300.683 kb on - strand, within Psyr_1149 at 1300.756 kb on + strand, within Psyr_1149 at 1300.862 kb on + strand, within Psyr_1149 at 1300.862 kb on + strand, within Psyr_1149 at 1300.862 kb on + strand, within Psyr_1149 at 1300.862 kb on + strand, within Psyr_1149 at 1300.862 kb on + strand, within Psyr_1149 at 1300.862 kb on + strand, within Psyr_1149 at 1300.863 kb on - strand, within Psyr_1149 at 1300.863 kb on - strand, within Psyr_1149 at 1300.863 kb on - strand, within Psyr_1149 at 1300.863 kb on - strand, within Psyr_1149 at 1300.863 kb on - strand, within Psyr_1149 at 1300.863 kb on - strand, within Psyr_1149 at 1300.982 kb on + strand, within Psyr_1149 at 1301.115 kb on + strand, within Psyr_1149 at 1301.115 kb on + strand, within Psyr_1149 at 1301.115 kb on + strand, within Psyr_1149 at 1301.138 kb on + strand at 1301.139 kb on - strand at 1301.235 kb on + strand at 1301.235 kb on + strand at 1301.235 kb on + strand at 1301.235 kb on + strand at 1301.236 kb on - strand at 1301.236 kb on - strand at 1301.236 kb on - strand at 1301.236 kb on - strand at 1301.236 kb on - strand at 1301.236 kb on - strand at 1301.236 kb on - strand at 1301.254 kb on + strand at 1301.255 kb on - strand at 1301.265 kb on + strand at 1301.266 kb on - strand at 1301.284 kb on + strand at 1301.284 kb on + strand at 1301.284 kb on + strand at 1301.284 kb on + strand at 1301.284 kb on + strand at 1301.285 kb on - strand at 1301.322 kb on + strand at 1301.885 kb on - strand, within Psyr_1150
Per-strain Table
Position Strand Gene LocusTag Fraction KB with capsaicin 0.25 mg/ml remove 1,299,281 - Psyr_1148 0.13 +0.2 1,299,309 - Psyr_1148 0.15 -1.0 1,299,525 - Psyr_1148 0.37 +0.0 1,299,716 + Psyr_1148 0.55 +0.5 1,299,717 - Psyr_1148 0.55 +0.7 1,299,776 + Psyr_1148 0.61 -1.2 1,299,776 + Psyr_1148 0.61 +0.2 1,299,777 - Psyr_1148 0.61 -1.8 1,299,777 - Psyr_1148 0.61 -0.4 1,299,777 - Psyr_1148 0.61 -0.5 1,299,777 - Psyr_1148 0.61 +0.7 1,299,777 - Psyr_1148 0.61 -0.8 1,300,018 + Psyr_1148 0.85 +0.2 1,300,040 + Psyr_1148 0.87 -0.3 1,300,040 + Psyr_1148 0.87 -0.1 1,300,041 - Psyr_1148 0.87 +0.5 1,300,064 + Psyr_1148 0.89 +0.2 1,300,064 + Psyr_1148 0.89 -1.0 1,300,065 - Psyr_1148 0.90 +1.2 1,300,103 + -0.6 1,300,103 + -0.1 1,300,103 + -2.8 1,300,103 + -0.6 1,300,104 - +0.5 1,300,104 - -1.0 1,300,317 + +0.3 1,300,319 + -0.0 1,300,319 + +0.7 1,300,319 + -0.1 1,300,320 - +1.1 1,300,320 - +0.7 1,300,361 + Psyr_1149 0.13 -2.0 1,300,362 - Psyr_1149 0.13 +1.8 1,300,412 + Psyr_1149 0.19 -0.8 1,300,412 + Psyr_1149 0.19 +0.7 1,300,413 - Psyr_1149 0.19 +0.8 1,300,413 - Psyr_1149 0.19 -0.4 1,300,413 - Psyr_1149 0.19 -1.1 1,300,454 + Psyr_1149 0.23 -0.2 1,300,454 + Psyr_1149 0.23 +0.3 1,300,454 + Psyr_1149 0.23 +0.2 1,300,575 - Psyr_1149 0.35 +1.2 1,300,575 - Psyr_1149 0.35 -0.3 1,300,593 - Psyr_1149 0.37 -1.2 1,300,682 + Psyr_1149 0.46 +2.4 1,300,683 - Psyr_1149 0.46 +0.2 1,300,756 + Psyr_1149 0.53 +0.3 1,300,862 + Psyr_1149 0.64 -0.5 1,300,862 + Psyr_1149 0.64 -0.9 1,300,862 + Psyr_1149 0.64 +0.1 1,300,862 + Psyr_1149 0.64 +0.6 1,300,862 + Psyr_1149 0.64 -1.9 1,300,862 + Psyr_1149 0.64 +1.3 1,300,863 - Psyr_1149 0.64 -0.1 1,300,863 - Psyr_1149 0.64 +0.5 1,300,863 - Psyr_1149 0.64 +0.2 1,300,863 - Psyr_1149 0.64 +0.4 1,300,863 - Psyr_1149 0.64 +1.3 1,300,863 - Psyr_1149 0.64 -2.6 1,300,982 + Psyr_1149 0.76 -0.2 1,301,115 + Psyr_1149 0.89 +1.4 1,301,115 + Psyr_1149 0.89 +0.7 1,301,115 + Psyr_1149 0.89 +0.2 1,301,138 + +0.4 1,301,139 - +0.7 1,301,235 + +0.8 1,301,235 + +0.2 1,301,235 + -1.1 1,301,235 + -0.6 1,301,236 - -0.3 1,301,236 - +0.1 1,301,236 - +0.3 1,301,236 - +1.1 1,301,236 - +0.5 1,301,236 - -0.6 1,301,236 - -0.0 1,301,254 + +0.1 1,301,255 - +1.0 1,301,265 + -1.4 1,301,266 - -0.3 1,301,284 + +0.2 1,301,284 + +0.9 1,301,284 + +0.7 1,301,284 + +0.5 1,301,284 + -0.9 1,301,285 - +0.3 1,301,322 + +0.3 1,301,885 - Psyr_1150 0.30 +0.7
Or see this region's nucleotide sequence