Experiment: KB with capsaicin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0169 and Psyr_0170 are separated by 177 nucleotides Psyr_0170 and Psyr_0171 are separated by 167 nucleotides Psyr_0171 and Psyr_0172 are separated by 16 nucleotides
Psyr_0169: Psyr_0169 - Hsp33 protein, at 183,940 to 184,842
_0169
Psyr_0170: Psyr_0170 - heat shock protein Hsp15, at 185,020 to 185,427
_0170
Psyr_0171: Psyr_0171 - EAL, at 185,595 to 186,377
_0171
Psyr_0172: Psyr_0172 - Protein of unknown function DUF785, at 186,394 to 186,870
_0172
Position (kb) 185
186 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 184.403 kb on + strand, within Psyr_0169 at 184.403 kb on + strand, within Psyr_0169 at 184.403 kb on + strand, within Psyr_0169 at 184.403 kb on + strand, within Psyr_0169 at 184.404 kb on - strand, within Psyr_0169 at 184.404 kb on - strand, within Psyr_0169 at 184.404 kb on - strand, within Psyr_0169 at 184.751 kb on + strand, within Psyr_0169 at 184.842 kb on + strand at 184.842 kb on + strand at 184.842 kb on + strand at 184.842 kb on + strand at 184.842 kb on + strand at 184.842 kb on + strand at 184.842 kb on + strand at 184.842 kb on + strand at 184.842 kb on + strand at 184.842 kb on + strand at 184.843 kb on - strand at 184.843 kb on - strand at 184.843 kb on - strand at 184.843 kb on - strand at 184.843 kb on - strand at 184.843 kb on - strand at 184.843 kb on - strand at 184.843 kb on - strand at 184.843 kb on - strand at 184.843 kb on - strand at 184.843 kb on - strand at 184.843 kb on - strand at 184.846 kb on + strand at 184.846 kb on + strand at 184.855 kb on + strand at 184.856 kb on - strand at 184.856 kb on - strand at 184.856 kb on - strand at 184.856 kb on - strand at 184.864 kb on + strand at 184.864 kb on + strand at 184.864 kb on + strand at 184.864 kb on + strand at 184.865 kb on - strand at 184.865 kb on - strand at 184.947 kb on + strand at 184.954 kb on + strand at 184.954 kb on + strand at 184.954 kb on + strand at 184.954 kb on + strand at 184.954 kb on + strand at 184.955 kb on - strand at 185.224 kb on + strand, within Psyr_0170 at 185.385 kb on + strand, within Psyr_0170 at 185.385 kb on + strand, within Psyr_0170 at 185.386 kb on - strand, within Psyr_0170 at 185.526 kb on + strand at 185.527 kb on - strand at 185.566 kb on + strand at 185.566 kb on + strand at 185.567 kb on - strand at 185.682 kb on + strand, within Psyr_0171 at 185.682 kb on + strand, within Psyr_0171 at 185.683 kb on - strand, within Psyr_0171 at 186.257 kb on + strand, within Psyr_0171 at 186.257 kb on + strand, within Psyr_0171 at 186.257 kb on + strand, within Psyr_0171 at 186.258 kb on - strand, within Psyr_0171 at 186.292 kb on + strand, within Psyr_0171 at 186.310 kb on - strand at 186.310 kb on - strand at 186.382 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with capsaicin 0.25 mg/ml remove 184,403 + Psyr_0169 0.51 -0.3 184,403 + Psyr_0169 0.51 -0.2 184,403 + Psyr_0169 0.51 -0.5 184,403 + Psyr_0169 0.51 -0.5 184,404 - Psyr_0169 0.51 -1.5 184,404 - Psyr_0169 0.51 +0.0 184,404 - Psyr_0169 0.51 -1.0 184,751 + Psyr_0169 0.90 +1.5 184,842 + -0.4 184,842 + +1.5 184,842 + -0.8 184,842 + +1.0 184,842 + -0.4 184,842 + -0.5 184,842 + +0.2 184,842 + +0.4 184,842 + -0.9 184,842 + -0.4 184,843 - +0.4 184,843 - +0.4 184,843 - -3.2 184,843 - -0.9 184,843 - -1.0 184,843 - -1.0 184,843 - +0.4 184,843 - -0.4 184,843 - -0.4 184,843 - -0.6 184,843 - +0.5 184,843 - -1.0 184,846 + -0.1 184,846 + -1.0 184,855 + +0.1 184,856 - +0.0 184,856 - +1.0 184,856 - -0.4 184,856 - -0.2 184,864 + -0.7 184,864 + -1.3 184,864 + +1.2 184,864 + -1.2 184,865 - +0.7 184,865 - +0.0 184,947 + +0.4 184,954 + +0.2 184,954 + +0.5 184,954 + -0.5 184,954 + +0.3 184,954 + -0.2 184,955 - +1.3 185,224 + Psyr_0170 0.50 -0.7 185,385 + Psyr_0170 0.89 -0.1 185,385 + Psyr_0170 0.89 -1.4 185,386 - Psyr_0170 0.90 -0.4 185,526 + -1.5 185,527 - -0.9 185,566 + -0.6 185,566 + +0.9 185,567 - -0.9 185,682 + Psyr_0171 0.11 -0.8 185,682 + Psyr_0171 0.11 -0.2 185,683 - Psyr_0171 0.11 +0.1 186,257 + Psyr_0171 0.85 -0.3 186,257 + Psyr_0171 0.85 -0.1 186,257 + Psyr_0171 0.85 -0.0 186,258 - Psyr_0171 0.85 -0.1 186,292 + Psyr_0171 0.89 -1.1 186,310 - +0.8 186,310 - +0.4 186,382 + -0.5
Or see this region's nucleotide sequence