Experiment: 3-methyl-2-butenol 0.31 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ftsY and yhhF are separated by 149 nucleotides yhhF and yhhL overlap by 11 nucleotides yhhL and yhhM are separated by 2 nucleotides yhhM and yhhN are separated by 140 nucleotides
b3464: ftsY - fused Signal Recognition Particle (SRP) receptor: membrane binding protein/conserved protein (NCBI), at 3,600,773 to 3,602,266
ftsY
b3465: yhhF - predicted methyltransferase (NCBI), at 3,602,416 to 3,603,012
yhhF
b3466: yhhL - conserved inner membrane protein (NCBI), at 3,603,002 to 3,603,271
yhhL
b3467: yhhM - hypothetical protein (NCBI), at 3,603,274 to 3,603,633
yhhM
b3468: yhhN - conserved inner membrane protein (NCBI), at 3,603,774 to 3,604,400
yhhN
Position (kb)
3602
3603
3604 Strain fitness (log2 ratio)
-2
-1
0
1 at 3602.144 kb on - strand at 3602.314 kb on + strand at 3602.314 kb on + strand at 3602.321 kb on - strand at 3602.324 kb on - strand at 3602.358 kb on + strand at 3602.358 kb on + strand at 3602.377 kb on + strand at 3602.377 kb on + strand at 3602.509 kb on - strand, within yhhF at 3602.509 kb on - strand, within yhhF at 3602.519 kb on - strand, within yhhF at 3602.519 kb on - strand, within yhhF at 3602.522 kb on - strand, within yhhF at 3602.522 kb on - strand, within yhhF at 3602.522 kb on - strand, within yhhF at 3602.592 kb on - strand, within yhhF at 3602.621 kb on - strand at 3602.634 kb on - strand, within yhhF at 3602.713 kb on - strand, within yhhF at 3602.736 kb on - strand, within yhhF at 3602.773 kb on - strand, within yhhF at 3602.780 kb on - strand, within yhhF at 3602.780 kb on - strand, within yhhF at 3602.783 kb on - strand, within yhhF at 3602.882 kb on - strand, within yhhF at 3602.907 kb on + strand, within yhhF at 3602.947 kb on - strand, within yhhF at 3602.992 kb on - strand at 3602.993 kb on + strand at 3603.015 kb on - strand at 3603.062 kb on - strand, within yhhL at 3603.062 kb on - strand, within yhhL at 3603.065 kb on + strand, within yhhL at 3603.065 kb on + strand, within yhhL at 3603.083 kb on - strand, within yhhL at 3603.134 kb on + strand, within yhhL at 3603.134 kb on + strand, within yhhL at 3603.135 kb on + strand, within yhhL at 3603.135 kb on + strand, within yhhL at 3603.143 kb on - strand, within yhhL at 3603.159 kb on - strand, within yhhL at 3603.225 kb on + strand, within yhhL at 3603.247 kb on - strand at 3603.279 kb on - strand at 3603.279 kb on - strand at 3603.314 kb on - strand, within yhhM at 3603.314 kb on - strand, within yhhM at 3603.316 kb on + strand, within yhhM at 3603.316 kb on + strand, within yhhM at 3603.339 kb on + strand, within yhhM at 3603.352 kb on + strand, within yhhM at 3603.357 kb on + strand, within yhhM at 3603.360 kb on - strand, within yhhM at 3603.410 kb on - strand, within yhhM at 3603.420 kb on + strand, within yhhM at 3603.423 kb on + strand, within yhhM at 3603.435 kb on - strand, within yhhM at 3603.470 kb on + strand, within yhhM at 3603.678 kb on + strand at 3603.726 kb on + strand at 3603.776 kb on + strand at 3603.812 kb on + strand at 3603.825 kb on + strand at 3603.825 kb on + strand at 3603.945 kb on + strand, within yhhN at 3603.945 kb on + strand, within yhhN at 3603.955 kb on - strand, within yhhN at 3603.960 kb on + strand, within yhhN at 3603.960 kb on + strand, within yhhN at 3603.968 kb on - strand, within yhhN at 3603.980 kb on - strand, within yhhN at 3603.980 kb on - strand, within yhhN at 3603.996 kb on + strand, within yhhN at 3603.996 kb on + strand, within yhhN
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-2-butenol 0.31 vol% remove 3,602,144 - -0.1 3,602,314 + -1.1 3,602,314 + -0.4 3,602,321 - +0.0 3,602,324 - -0.0 3,602,358 + -1.2 3,602,358 + +0.4 3,602,377 + -0.2 3,602,377 + -0.8 3,602,509 - yhhF b3465 0.16 -0.2 3,602,509 - yhhF b3465 0.16 -1.1 3,602,519 - yhhF b3465 0.17 +0.1 3,602,519 - yhhF b3465 0.17 -0.5 3,602,522 - yhhF b3465 0.18 -0.5 3,602,522 - yhhF b3465 0.18 -0.5 3,602,522 - yhhF b3465 0.18 -0.6 3,602,592 - yhhF b3465 0.29 -2.2 3,602,621 - +1.4 3,602,634 - yhhF b3465 0.37 +0.2 3,602,713 - yhhF b3465 0.50 -1.0 3,602,736 - yhhF b3465 0.54 -0.5 3,602,773 - yhhF b3465 0.60 -1.8 3,602,780 - yhhF b3465 0.61 -0.7 3,602,780 - yhhF b3465 0.61 -0.7 3,602,783 - yhhF b3465 0.61 -0.6 3,602,882 - yhhF b3465 0.78 +1.1 3,602,907 + yhhF b3465 0.82 -0.9 3,602,947 - yhhF b3465 0.89 -0.1 3,602,992 - +0.2 3,602,993 + -0.0 3,603,015 - +1.0 3,603,062 - yhhL b3466 0.22 -0.4 3,603,062 - yhhL b3466 0.22 -1.0 3,603,065 + yhhL b3466 0.23 -0.8 3,603,065 + yhhL b3466 0.23 -0.4 3,603,083 - yhhL b3466 0.30 -0.6 3,603,134 + yhhL b3466 0.49 -1.3 3,603,134 + yhhL b3466 0.49 +0.9 3,603,135 + yhhL b3466 0.49 +0.2 3,603,135 + yhhL b3466 0.49 +0.4 3,603,143 - yhhL b3466 0.52 -0.3 3,603,159 - yhhL b3466 0.58 +0.2 3,603,225 + yhhL b3466 0.83 -0.3 3,603,247 - -0.7 3,603,279 - +0.1 3,603,279 - +0.1 3,603,314 - yhhM b3467 0.11 +0.2 3,603,314 - yhhM b3467 0.11 +0.0 3,603,316 + yhhM b3467 0.12 +0.1 3,603,316 + yhhM b3467 0.12 -0.7 3,603,339 + yhhM b3467 0.18 +0.9 3,603,352 + yhhM b3467 0.22 +0.3 3,603,357 + yhhM b3467 0.23 +0.6 3,603,360 - yhhM b3467 0.24 -0.5 3,603,410 - yhhM b3467 0.38 -0.9 3,603,420 + yhhM b3467 0.41 +1.1 3,603,423 + yhhM b3467 0.41 -1.0 3,603,435 - yhhM b3467 0.45 +0.1 3,603,470 + yhhM b3467 0.54 +0.3 3,603,678 + +0.5 3,603,726 + +0.0 3,603,776 + +0.5 3,603,812 + +1.4 3,603,825 + -1.0 3,603,825 + +1.0 3,603,945 + yhhN b3468 0.27 +0.0 3,603,945 + yhhN b3468 0.27 -0.2 3,603,955 - yhhN b3468 0.29 +0.8 3,603,960 + yhhN b3468 0.30 +0.5 3,603,960 + yhhN b3468 0.30 +0.2 3,603,968 - yhhN b3468 0.31 +0.9 3,603,980 - yhhN b3468 0.33 -0.3 3,603,980 - yhhN b3468 0.33 -0.7 3,603,996 + yhhN b3468 0.35 +0.0 3,603,996 + yhhN b3468 0.35 +0.8
Or see this region's nucleotide sequence